Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (7): 1387-1391.doi: 10.3864/j.issn.0578-1752.2012.07.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

IL-8 Gene SNP-180A/G Affects Significantly Somatic Cell Score in Chinese Holstein Cow

 JI  De-Jun, CHEN  Ren-Jin, YANG  Zhang-Ping, ZHU  Guo-Qiang, MAO  Yong-Jiang, CHEN  Ying, LI  Rui   

  1. 1.扬州大学动物科学与技术学院，江苏扬州 225009
    2.扬州大学兽医学院，江苏扬州 225009
  • Received:2011-06-20 Online:2012-04-01 Published:2011-09-20

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Abstract: 【Objective】 Bovine interleukin-8 (IL-8) is one of the major factors recruiting neutrophils during cow mastitis and causing rapid increase of somatic cell counts (SCC). Knowledge of IL-8 polymorphisms would be helpful to genetic selection of low SCC. 【Method】 The SNPs in the 5′ upstream of bovine IL-8 gene were investigated in 610 Chinese Holstein cows from 30 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique, and milking traits and somatic cell score (SCS) were measured and their relationship was analyzed using a mixed animal model. 【Result】 The results showed that one SNP, A(-180)G, was detected, determining three genotypes, GG, GA and AA with frequencies of 0.41, 0.43 and 0.16, respectively. The SNP showed significant effect on SCS, with GG having a significantly lower SCS than GA or AA (P＜0.01), while this SNP had no effect on test-day milk yield, test-day milk fat content, test-day milk protein content, and 305 d corrected milk yield. 【Conclusion】The findings demonstrated that the SNP -180A/G exerted significant effect on SCS.

Key words: Chinese Holstein cow, IL-8 gene, SCS, SNP, milking traits

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