Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (10): 2109-2117.doi: 10.3864/j.issn.0578-1752.2011.10.016

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Distribution of Stropharia rugoso–annulata Polysaccharides Molecular Weight and Component Sugar

 CHEN  Jun-Chen, WENG  Min-Jie, LAI  Pu-Fu, LI  Yi-Bin, ZHOU  Xue-Hua, SHEN  Heng-Sheng   

  1. 1.福建省农业科学院农产品加工研究中心/福建省农业科学院农业工程技术研究所，福州 350003
  • Received:2010-09-01 Online:2011-05-15 Published:2011-03-04

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Abstract: 【Objective】The aim of this paper was to extract and isolate Stropharia rugoso-annulata polysaccharides and analyze the molecular weight and component sugar of polysaccharides.【Method】In this paper, polysaccharide from Stropharia rugoso-annulata was obtained by water extraction method, which the molecular weight distribution and changes during extraction course was investigated by using high performance liquid chromatography. The component sugar of the polysaccharides were also measured by infrared spectrum and nuclear magnetic resonance methods. 【Result】The molecular weight distribution of water soluble polysaccharides from Stropharia rugoso-annulata was about 22 kD, α and β glucosidic bonds were co-exist in polysaccharides molecule which contain 5 kinds of monosaccharides. The main monosaccharide was pyranose. D-fructose, D-glucose and D-xylose exist in polysaccharides molecule. 【Conclusion】 The detection method with hydrochloric acid solution as the mobile phase can reflect more real molecular weight distribution of polysaccharide from Stropharia rugoso-annulata, and Stropharia rugoso-annulata polysaccharide is a heterosaccharide which is composed of five monosaccharides.

Key words: Stropharia rugoso-annulata, polysaccharides, molecular weight

[1]王小东, 毛慧玲, 王顺启. 真菌多糖的研究进展. 江西科学, 2005, 23(3): 243-247.

Wang X D, Mao H L, Wang S Q. Research advance of fungus polysaccharides. Jiangxi Science, 2005, 23(3) 3: 243-247. (in Chinese)

[2]赵国华, 陈宗道, 李志孝, 阚建全. 活性多糖的研究进展. 食品与发酵工业, 2001, 27(7): 45-48.

Zhao G H, Chen Z D, Li Z X, Kan J Q. Advance in bioactive polysaccharide. Food and Fermentation Industries, 2001, 27(7):45-48. (in Chinese)

[3]陶文沂, 敖宗华, 许泓瑜, 许正宏. 药食用真菌生物技术. 北京: 化学工业出版社生物•医药出版分社, 2007.

Tao W Y, Ao Z H, Xu H Y, Xu Z H. Biological Technology of Medicinal Edible Fungi. Beijing: Biological Medicine　Branch　of Chemical Industry Press, 2007. (in Chinese)

[4]李小定, 荣建华, 吴谋成. 真菌多糖生物活性研究进展. 食用菌学报, 2002, 9 (4): 50-58.

Li X D, Rong J H, Wu M C. Recent advances in the studies on bio-activities of fungus polysaccharides. Acta Edulis Fungi, 2002, 9 (4): 50-58. (in Chinese)

[5]陈爱葵, 易  广, 李爱群. 食用菌在提高人体免疫力方面的功效. 中国食用菌, 2004, 23 (3) : 7-9.

Chen A K, Yi G, Li A Q. Edible fungus effects on enhancing human immunity. Edible Fungi of China, 2004, 23 (3): 7-9. (in Chinese)

[6]李月梅. 食用菌的功能成分与保健功效. 食品科学, 2005, 26(8): 517-521.

Li Y M. Function components and health function of edible fungi. Food Science, 2005, 26(8): 517-521. (in Chinese)

[7]Hawksworth D L, Kirk P M, Sutton B C, Pegler D N. Ainsworth and bisby's dictionary of the fungi. CAB International, 1995.

[8]萨仁图雅, 图力古尔. 大球盖菇研究进展. 食用菌学报, 2005, 12(4): 57-64.

Wan S, Bau T. Advances in the study on Stropharia rugoso -annulata. Acta Edulis Fungi, 2005, 12(4): 57-64. (in Chinese)

[9]黄年来. 大球盖菇的分类地位和特征特性. 食用菌, 1995, 17(6): 11.

Huang N L. Taxonomic position and characteristics of Stropharia rugoso-annulata. Edible Fungi, 1995, 17 (6): 11. (in Chinese)

[10]佘冬芳, 樊卫国, 徐彦军. 白灵菇和大球盖菇的营养分析与比较. 食用菌, 2007, 29(4): 57-58.

She D F, Fan W G, Xu Y J. Analysis and comparison on the nutrients in Pleurotus nebrodensis and Stropharia rugoso-annulata. Edible Fungi, 2007, 29(4): 57-58. (in Chinese)

[11]王晓炜, 詹  巍, 陶明煊, 吉钟山, 刘  杨, 王  峰, 程光宇. 大球盖菇营养成分、抗氧化活性物质分析. 食用菌, 2007, 29(6): 62-63.

Wang X W, Zhan W, Tao M X, Ji Z S, Liu Y, Wang F, Cheng G Y. Analysis on nutrient components and antioxidant components of Stropharia rugoso-annulata. Edible Fungi, 2007, 29(6): 62-63. (in Chinese)

[12]段永刚. 大球盖菇营养品质分析、黄酮类化合物提取及应用研究[D]. 福州: 福建农林大学, 2010.

Duan Y G. Nutritional quality analyse, extraction and application of flavonoids of Stropharia rugoso-annulata farlow[D]. Fuzhou: Fujian Agriculture and Forestry University, 2010. (in Chinese)

[13]许  峰. 大球盖菇富硒液体培养条件优化及抗氧化能力初步研究[D]. 泰安: 山东农业大学, 2006.

Xu F. Optimizing liquid cultural conditions of Stropharia rugoso- annulata accumulated selenium and studies on its antioxidative activities[D]. Taian: Shandong Agricultural University, 2006. (in Chinese)

[14]Scheibner K, Hofrichter M, Herre A, Michels J. Screening for fungi intensively mineralizing 2, 4, 6-treinitrotoluene. Applied Microbiology and Biotechnology, 1997, 47: 452-457.

[15]Steffen K T, Hofrichter M, Hatakka A. Mineralisation of 14C-labelled synthetic lignin and ligninolytic enzyme activities of litter- decomposing basidiomycetous fungi. Applied Microbiology and Biotechnology, 2000, 54: 819-825.

[16]黄年来. 中国大型真菌原色图鉴. 北京: 中国农业出版社, 1998.

Huang N L. Unbleached Pictorialial Yearbook of Macro-fungi　in China. Beijing: China Agriculture Press, 1998. (in Chinese)

[17]杜  巍, 李元瑞, 袁  静. 食药用菌多糖生物活性与结构的关系.中国食用菌, 2001, 21(2) : 28-30.

Du W, Li Y R, Yuan J. The relation to polysaccharide active and structure of edible and pharmaceutical fungi. Edible Fungi of China, 2002, 21(2):28-30. (in Chinese)

[18]王晓炜. 大球盖菇营养成分分析、多糖提取分离及抗氧化作用研 究[D]. 南京: 南京师范大学, 2007.

Wang X W. Nutrition components analyse, extraction and antioxidant properties of polysaccharide of Stropharia rugoso-annulata[D]. Nanjing: Nanjing Normal University, 2007. (in Chinese)

[19]陶明煊, 王  峰, 王晓炜, 金  鹏, 程光宇. 大球盖菇多糖对小鼠心脏抗氧化作用研究. 食品科学, 2007, 28(9) : 529-532.

Tao M X, Wang F, Wang X W, Jin P, Cheng G Y. Study on antioxidant of Stropharia rugoso-annulata polysaccharide on mouse heart. Food Science, 2007, 28(9) : 529-532. (in Chinese)

[20]庞振凌, 田  龙. 大球盖菇多糖发酵工艺优化研究. 江苏农业科学, 2007(1): 157-158.

Pang Z L, Tian L. Study on fermentation technical optimization of polysaccharide from Stropharia rugoso-annulata. Jiangsu Agricultural Sciences, 2007(1): 157-158. (in Chinese)

[21]Duenas Chasco M T, Rodriguez-Carvajal M A, Mateo P T, Fanko-Rodriguez G, Espartero J, Irastorza-Iribas A, Gil-Serrano A M. Structural analysis of the exopolysaccharide produced by Pediococcus damnosus. Carbohydrate Resource, 1997, 303: 453-458.

[22]Leontein K, Lindberg B, Lonngren J. Assignment of absolute configuration of sugars by glc of their acetylated glycosides formed. Carbohydrate Research, 1978, 62: 359-362.

[23]Gerwig G J, Kamerling J P, Vliegenthart J F G. Determination of the absolute configuration of monosaccharide in complex carbohydrates by capillary G.L.C. Carbohydrate Research, 1979, 77: 1-7.

[24]Whittaker D V, Parolis L A, Parolis H. Structural elucidation of the capsular polysaccharide produced by Escherichia coli O20:K84:H26. Carbohydrate Research, 1994, 262(2): 323-334.

[25]Holmbeck S M A, Petillo P A, Lerner L E. The solution conformation of hyaluronan: A combined NMR and molecular dynamics study. Biochemistry, 1994, 33(47): 14246-14255.

[26]Perlin A S, Ng Ying Kin N M K, Bhattacharjee S S, Johnson L F. The 13C Fourier transform spectrum of Heparin. Evidence for a biose repeating sequence of residues. Canadian Journal of Chemistry, 1972, 50: 2437-2441.
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