猪骨和皮脂性状相关ESTs的SNPs筛查与关联分析

doi:10.3864/j.issn.0578-1752.2011.16.013

Abstract

Abstract: 【Objective】 QTLs are located in several chromosomes. In order to provide a reference, new candidate genes of bone and sebum traits of pigs were screened.【Method】One hundred and seventy-seven ESTs that may be associated with bone and sebum traits have been searched from NCBI and 21 SNPs were screened by cDNA pool sequencing in 9 ESTs. Four ESTs were located by RH (radiation hybrid). 【Result】 It was discovered that 6 SNPs that are related to bone and sebum traits actually exist in the F2 crossbred population of the Landrace pigs and Lantang pigs after the correlation analysis. E53 is extremely significantly correlated with rear bone (ReB) (P＜0.01)and significantly correlated with bone propotion (BP), fore bone (ForeB) and sebum fat propotion (SFP) (P＜0.05).E82 is extremely significantly correlated with mid-bone (MidB), mid-sebum fat (MidSF) (P＜0.01) and significantly correlated with bone propotion (BP), sebum fat propotion (SFP) and fore sebum fat (ForeSF) (P＜0.05). The differences of E36 in rear sebum fat (ReSF) and fore bone (ForeB), E42 in mid-sebum fat (MidSF) and rear sebum fat (ReSF), E54 in mid-bone (MidB), E87 in fore bone (ForeB) and sebum fat propotion (SFP) reached the remarkable level (P＜0.05). E36 is located on 10q13. E53 is located on 4q21-23. E54 is located on SWC19 of the chromosome 10 without the specific position. E87 is located on 9p21.【Conclusion】E53 and E82 need to be studied further as new candidate genes which affect the bone and sebum traits of pigs.

Key words: ESTs, SNPs, Bone Traits, Sebum Traits

QIN Li-Li, CAI Geng-Yuan, ZHANG Hao, CHEN Yao-Sheng, LI Jia-Qi, WANG Chong. SNPs Screening and Correlation Analysis of ESTs Associated With Bone and Sebum Traits of Pigs[J].Scientia Agricultura Sinica, 2011, 44(16): 3403-3412.

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SNPs Screening and Correlation Analysis of ESTs Associated With Bone and Sebum Traits of Pigs

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