Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (16): 3447-3454 .doi: 10.3864/j.issn.0578-1752.2010.16.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Detection of Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Pet Animals

ZHU Heng-qian, LIAO Xiao-ping, CHEN Chao-xi, WANG Xiu-mei, SUN Jian, SUN ying,LI Liang, ZHANG Mei-jun, LIU Ya-hong   

  1. (华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室)
  • Received:2009-12-25 Revised:2010-04-01 Online:2010-08-15 Published:2010-08-15
  • Contact: LIU Ya-hong

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Abstract:

【Objective】 The plasmid-mediated quinolone resistance PMQR genes of qnr, qepA and aac (6′)-Ib-cr in clinical isolates of Escherichia coli from pet animals (dogs and cats) in Guangzhou city were detected. 【Method】 Susceptibility of 164 strains to 15 antimicrobial agents were determined by agar disc dilution method. All of the 164 strains were screened for the qnr, qepA, aac (6′)-Ib-cr genes by PCR. Genetic fingerprints of the clinical isolates were established and analyzed. 【Results】 The isolates showed a high rate of resistance to antimicrobial agents widely used in veterinary clinical medicine and most of the them showed multiple-resistance(5 to 14 drugs). Three of these 164 specimens were detected containing qnrA, qnrB and qnrS genes, and two of which also posses aac(6′)-Ib-cr gene. Fingerprints could be amplifled from all the positive strains, and strains with more than two PMQR genes were distributed mainly in the B and C gene types. 【Conclusion】 Escherichia coli isolated from pet animals in Guangzhou city showed a high level multiple-resistance to widely used drugs. The result of the research showed a more widespread dissemination of resistance to quinolones in pet animal veterinary clinic of Guangzhou city, and the ability to generate PMQR gene of isolates is closely to their gene-type.

Key words: pet animals, Escherichia coli, plasmid mediate, quinolones, resistance gene

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