Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (04): 798-801 .doi: 10.3864/j.issn.0578-1752.at-2004-2998

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

The Evidence of the Origin of a Polymorphic High Molecular Weight Protein in Sow Milk

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  1. 南京农业大学农业部动物生理生化重点开放实验室
  • Received:2004-12-01 Revised:1900-01-01 Online:2006-04-10 Published:2006-04-10

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Abstract: 【Objective】 High molecular weight proteins (HMWP) in sow milk showed four variants of A, B, C, and D by SDS-PAGE, the origin of HMWP-B was studied by this paper.【Method】The experiment is carried out by immunoblotting with the rabbit anti- HMWP-B polyclonal antibody. 【Results】The results show that there is no HMWP-B in the serum of the sow, and suggest that HMWP-B is synthesized and secreted by mammary cell. Besides, the result of immunoblotting of cow and rabbit milk with the rabbit anti- HMWP-B polyclonal antibody indicated that there is no homologous protein of HMWP-B in cow and rabbit milk. So, it was presumed that HMWP-B might only exit in sow milk. 【Conclusion】The result of this study provides a proof for the hypothesis that HMWP-B is not originated from the blood.

Key words: Sow milk, High molecular weight protein (HMWP), Origin

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