Abstract: 【Objective】Sharp eyespot (Rhizoctonia cerealis) is one of the important diseases in wheat production in China. Due to lack of knowledge on the inheritance of resistance, little progress has been made in wheat breeding for sharp eyespot resistance. 【Method】In this study, the newly evaluated ARz and Niavt 14 were used as sharp eyespot resistant donors, and crossed with widely released cultivars such as Yangmai 158, respectively. At the meantime, ARz/Yangmai 158 genetic population was developed using SSD method with 137 F6 RILs. The 137 RILs and the parents were inoculated with the fungi culture R-46 in F6 using cultured toothpick method and seedbed method, respectively, in 2003.【Result】Novel germplasms, such as 02P12 and 02P315, with multiple resistance and promising agronomic traits were developed. The RIL population showed normal distribution of disease index, with disease index 29.8%-64.4% in cultured toothpick method and 25%-75% in seedbed method, indicating the resistance is a quantitative trait. By using marker regression analysis, 11 SSR markers associated with resistant QTL were found based on the disease index data, explaining 5.0%-13% of phenotypic variance. Among them, Xgwm247, Xgdm67, Xwmc94, and Xgwm437 were detectable in both inoculating methods. QTL mapping of resistance to sharp eyespot was conducted using MapManager QTXb17, 14 linkage groups were established. Two QTLs on chromosome 7D related to the resistance were found by composite interval mapping analysis. One QTL between Xgdm67 and Xbarc172 could been detected, explaining 14.36% and 12.3% of the phenotypic variance, in cultured toothpick and seedbed inoculation conditions respectively. Whereas the another QTL between Xwmc94 and Xwmc273.2 was only been found under the toothpick inoculation, explaining 14.68% of the phenotypic variance. 【Conclusion】 It was deduced that there is a major QTL conferring to resistance to sharp eyespot on 7D in wheat.

Key words: Wheat (Triticum aestivum L.), Sharp eyespot, Germplasm development, QTL analysis