Scientia Agricultura Sinica ›› 2004, Vol. 37 ›› Issue (10): 1474-1480 .

• PLANT PROTECTION • Previous Articles     Next Articles

Cloning and Mapping of cDNA from Cotton Induced by Toxin of Verticillium dahliae

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  1. 中国农业科学院
  • Received:2003-10-16 Revised:1900-01-01 Online:2004-10-20 Published:2004-10-20

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Abstract: In order to obtain the resistant-related candidate genes from upland cotton，response genes were induced by toxin of V. dahliae in upland cotton which display superior wilt tolerance. Total RNA was isolated from the seedling roots of upland cotton (Gossypium hirsutum L.var. Zhongmian12) after 24 h of induction by toxin of the pathogen V. dahliae strain V991, double strand cDNA was synthesized by PCR according to the procedure of the SmartTM cDNA Synthesis Kit(CLONTECH Co.), 180 clones were abtained by the method of Suppression Subtractive Hybridization,15 cDNA clones were identified to be positive by Reverse Northern Blot, which was named as response gene induced by toxin of Verticillium dahliae, for short as Vdrg. Sequence analysis and cotton genome locating of Vdrg using F2 populations derived from Pima S6(G. barbardens) ×Acala Maxxa (G. hirsutum) were also carried out in this studies.

Key words: Upland cotton (G. hirsutum L.), Cotton Verticillium wilt, cDNA, Cloning and mapping

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