Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (12): 2474-2480 .doi: 10.3864/j.issn.0578-1752.at-2004-2767

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION • Previous Articles     Next Articles

Analysis on Microbial Diversity of Different Agricultural Soils by Using Molecular Biology Technique

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  1. 国立台湾大学园艺学研究所
  • Received:2004-09-14 Revised:2005-08-15 Online:2005-12-10 Published:2005-12-10

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Abstract: The microbial diversity and evenness in three different soil samples: compost-treated, chemical fertilizer-treated and control group soil samples were studied. The 16S rRNA gene cloning and restriction fragment length polymorphism (RFLP) were used for the comparison of soil microbe community. The optimization of RFLP digestion was firstly carried out, and AciI, BstUI, RsaI were optimal restriction enzymes for the digestion. The diversity indexes of soil microbes were also calculated and the indices of three treatments: control, compost, and chemical fertilizer-treated soils, are 0.990, 0.986, and 0.962 for D value, respectively; and 0.979, 0.977, and 0.931 for evenness, respectively.

Key words: Compost, Chemical fertilizer, Soil microorganism, Diversity, Evenness

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