Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (12): 2542-2548 .doi: 10.3864/j.issn.0578-1752.at-2005-6217

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Identification of FXPRLamide Family Neuropeptides from the Japanese Oak Silkworm, Antheraea yamamai by Using Immunocytochemistry Methods

,   

  1. 合肥工业大学 生物工程系
  • Received:2005-10-20 Revised:1900-01-01 Online:2005-12-10 Published:2005-12-10

PDF

740

Abstract: In present study, zooblooting, ELISA and whole-mount immunocytochemistry methods were used to identify the FXPRLamide family neuropeptides from the Japanese oak silkworm, Antheraea yamamai. The results showed that the genomic DNA from Antheraea yamamai showed positive bands after hybridized with the fragment of DH-PBAN cDNA from Samia cynthia ricini, which labeled with [ -32P]-dCTP. The SG showed highest FXPRLamide peptides titer in neural organs. Using an antiserum against H. armigera PBAN, PBAN-like immunoreactivity was detected in the SG and TG of Antheraea yamamai by whole-mount immunocytochemistry, and there were three clusters cells in the SG which shows positive PBAN-like immunoreactivity. The titers of FXPRLamide peptides immunoreactivity in the hemolymph kept a steady level. When pupation, the titer increased promptly, but decreased to a low level after early pupal stage. The above results demonstrated the existence of FXPRLamide family peptides in Antheraea yamamai, but its function need to be further investigated in the future.

Key words: Antheraea Yamamai, FXPRLamide family neyropeptides, immunocytochemistry, identification

[1] SUN YaLing, WANG QingHua, SHU Rui, YUE LiXin, WANG ZhenBao, LI ZhaoXia, GAO LiMin, CHENG Hong, FU ZaiQiu, HUO YuMeng. Production and Characterization of Interspecific Hybrids Between Allium fistulosum and Allium przewalskianum Regel [J]. Scientia Agricultura Sinica, 2026, 59(3): 619-636.
[2] HE ZhiLin, SUN CuiXia, YUE HongLi, TAN YueXia, ZHANG YaoHai, WANG FuSheng, LIU SiTao, JIANG Dong. Genetic Diversity Analysis and GWAS of Alloocimene Based on Resequencing of Citron, Lemon Germplasm Resources [J]. Scientia Agricultura Sinica, 2026, 59(2): 386-401.
[3] XU DuoDuo, DU QianQian, ZHAO LiXiang, LI Yan, HUANG Gan, LI YongHua, LU JiuXing. Genome-Wide Analysis of AP2/ERF Transcription Factors in Peony [J]. Scientia Agricultura Sinica, 2025, 58(23): 5031-5045.
[4] ZHANG ZhiChun, HE XiaoYi, HE ChengJun, GU DongLin, ZHONG Lian, GOU Yan, GAO BiXing, GENG Zhao, LI XiaoHong, CHEN SiKai. Comparative Analysis of Volatile Oil Chemical Constituents from Zanthoxylum bungeanum Seeds in Yanyuan and Hanyuan Based on GC-MS Technology [J]. Scientia Agricultura Sinica, 2025, 58(23): 5057-5070.
[5] CAI HaiLin, HUANG XinRong, ZENG WeiAi, PENG ZhiXin, ZHAO AJuan, BO ChunBin, XIANG ShiPeng, GAO PengCheng, ZHANG ChengSheng, ZHAO DongLin. Structure and Herbicidal Activity of 3,5-Dimethylorsellinic Acid-Derived Meroterpenoids from Penicillium sclerotiorum HY5 [J]. Scientia Agricultura Sinica, 2025, 58(22): 4673-4687.
[6] SU XiaoMei, YANG ZongHui, LIU ShuMei, ZHANG ZongJie, LÜ HongJun, HOU LiXia. Development and Application of A KASP Marker-Based Identification System for Tomato Varieties [J]. Scientia Agricultura Sinica, 2025, 58(22): 4746-4756.
[7] WU ShuYu, HENG YanFang, YU TaiFei, WANG ShiJia, YU SiJia, LI Yuan, HU Zheng, ZHANG Hui, SUN XianJun, LI Liang, JIANG QiYan. Identification of Salt Tolerance in Maize Natural Populations at the Seedling Stage and Analysis of Salt Tolerance-Associated Genes [J]. Scientia Agricultura Sinica, 2025, 58(20): 4085-4099.
[8] CHEN YongXian, CHEN RuiJiang, DU YiZhi, ZHU JunJie, CHEN WanXia, ZHAO ZiHan, WANG JiChun, DU Kang, ZHANG Kai. Screening and Identification of Drought-Tolerant Sweet Potato Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(2): 214-237.
[9] PAN Yuan, WANG De, LIU Nan, MENG XiangLong, DAI PengBo, LI Bo, HU TongLe, WANG ShuTong, CAO KeQiang, WANG YaNan. Evaluation of the Effectiveness of Two High-Throughput Sequencing Techniques in Identifying Apple Viruses and Identification of Two Novel Viruses [J]. Scientia Agricultura Sinica, 2025, 58(2): 266-280.
[10] YI ZeHui, WANG Ying, SONG HuiXia, ZHAO Jing, MAO LiPing. Genome-Wide Identification and Expression Analysis of Peroxiredoxins Gene Family in Asparagus officinalis [J]. Scientia Agricultura Sinica, 2025, 58(18): 3728-3743.
[11] LI YaYu, WANG XinLiang, ZHOU JinHuan, LI ChuXin, LI JiaXin, TIAN XuBin, SONG Zhen. Construction and Infectivity Identification of Genome-Length cDNA of Citrus Psorosis Virus [J]. Scientia Agricultura Sinica, 2025, 58(17): 3451-3460.
[12] ZHAO YuMeng, LI GuangXu, DAI QiDong, LIU JiaCheng, ZHAO HaiJuan, ZHANG YuJun, YANG Hua, LIU Shuo. Isolation and Identification of Pathogen of Plum Bacterial Shot Hole and Laboratory Test of Bactericide Toxicity [J]. Scientia Agricultura Sinica, 2025, 58(12): 2358-2370.
[13] WEI NaiCui, TAO JinBo, YUAN MingYang, ZHANG Yu, KAI MengXiang, QIAO Ling, WU BangBang, HAO YuQiong, ZHENG XingWei, WANG JuanLing, ZHAO JiaJia, ZHENG Jun. Seedling Characterization and Genetic Analysis of Low Phosphorus Tolerance in Shanxi Varieties [J]. Scientia Agricultura Sinica, 2024, 57(5): 831-845.
[14] CHEN XiHong, CAI Wei, YU Yun, LI Min, WANG NianWu, DU ZhenGuo, SHEN JianGuo, GAO FangLuan. Identification of Tea Plant Viruses in Fujian Province and Establishment of Multiplex PCR Detection Assay [J]. Scientia Agricultura Sinica, 2024, 57(4): 698-710.
[15] LI XinLei, SUN JiuYing, YANG Cheng, CHENG Ning, WANG KaiYue, WANG HuanHuan, CHENG XueJiao, ZHAO Jian, SUN YingFeng. Isolation, Identification and Genetic Variation of a Three-Lineage Strain Recombined Porcine Reproductive and Respiratory Syndrome Virus [J]. Scientia Agricultura Sinica, 2024, 57(24): 4978-4989.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd