高效三甲胺脱除菌株的筛选及生长条件优化

doi:10.3864/j.issn.0578-1752.2026.08.014

Abstract

Abstract:

【Background】Trimethylamine (TMA) is a typical nitrogen-containing amine organic pollutant, and one of the main malodorous gases causing odor pollution in animal husbandry. This gas is a harmful gas with a strong fishy odor. It not only causes point-source pollution in farming areas, but also its long-term exposure poses potential health risks to humans, attracting people's attention in recent years. 【Objective】This study aimed to screen high-efficiency TMA-removing strains with strong degradation ability by collecting large amounts of animal manure, for providing excellent strains for the prevention and control of TMA pollution in livestock farming. 【Method】In this study, efficient TMA-removing strains were screened from the manures of pigs and cows by the methods of enrichment culture, isolation, purification, and deodorization ability measurement. Then the strains were identified using morphological observation and 16S rRNA gene analysis to determine their taxonomic characteristics. On this basis, the effects of different pH values (5.0, 6.0, 7.0, 8.0, and 9.0), carbon sources (glucose, sucrose, fructose, maltose, and xylose), salinities (0, 0.5%, 1.0%, 1.5%, and 2.0%), and inoculum concentrations (1%, 5%, 10%, 15%, and 20%) on bacterial biomass were further investigated to define the optimal growth conditions for the strains. Moreover, the actual deodorization effect of the efficient strains was verified by investigating the release concentration and removal rate of TMA during the initial stage of feces fermentation. 【Result】(1) A total of 10 TMA-removing strains, named T1 to T10, were isolated from livestock manure, among which strain T2 (Bacillus subtilis) and strain T3 (Acinetobacter sp.) exhibited the highest removal abilities of TMA. The TMA-removing rates after 48 h of incubation reached 26.91% for T2 and 29.59% for T3, which were higher than those of the other eight strains (P < 0.05). (2) Different pH values, carbon sources, salinities, and inoculum concentrations exerted varying effects on the growth of strains T2 and T3. Optimal growth conditions for T2 were as follows: pH=6.0, glucose as carbon source, 0.5% NaCl, and inoculum concentration of 1%; for T3, pH=8.0, sucrose as carbon source, 0.5% NaCl, and inoculum concentration of 1%. Under these optimal conditions above, the strains exhibited the fastest growth rate and achieved the maximum biomass. (3) The verification test of deodorization effect showed that when the feces were supplemented with T2 or T3, the average release amounts of TMA were lower than those in the control group (P < 0.01), and the highest removal rates reached 42.65% for T1 and 39.63% for T2.【Conclusion】Bacillus subtilis (T2) and Acinetobacter sp. (T3) isolated in this study had a high-efficiency ability to remove TMA, and would possess potential utilization value in the field of livestock manure treatment and control of pollution in animal husbandry.

Key words: trimethylamine, odor, Bacillus subtilis, Acinetobacter sp., condition optimization

LIU YuQing, ZHANG Lu, LI JianJie, YANG HaiTong, SONG LianJie, LI BoSen, GAO YuHong, ZHANG HuiWen, SUN XinSheng. Isolation of Efficient Trimethylamine-Removing Strains and Optimization of Growth Conditions[J].Scientia Agricultura Sinica, 2026, 59(8): 1797-1808.

Figures/Tables 12

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Table 1

Fig. 6

Fig. 7

Fig. 8

Fig. 9

Table 2

Table 3

References 48

[1]	杨保留. 当前畜牧养殖环境污染的现状及治理对策. 中兽医学杂志, 2021(9): 93-94.
	YANG B L. Present situation and control countermeasures of environmental pollution in animal husbandry. Chinese Journal of Traditional Veterinary Science, 2021(9): 93-94. (in Chinese)
[2]	陈贺亮. 畜牧养殖环境污染的不同类型及其治理对策研究. 畜牧兽医科技信息, 2024(8): 65-68.
	CHEN H L. Study on different types of environmental pollution in animal husbandry and its control countermeasures. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2024(8): 65-68. (in Chinese)
[3]	车大璐, 赵俐辰, 程素彩, 刘爱瑜, 李晓宇, 赵寿培, 王健诚, 王媛, 高玉红, 孙新胜. 垫料床对育肥羔羊生长性能和臭气排放的影响. 中国农业科学, 2022, 55(24): 4943-4956. doi: 10.3864/j.issn.0578-1752.2022.24.012.
	CHE D L, ZHAO L C, CHENG S C, LIU A Y, LI X Y, ZHAO S P, WANG J C, WANG Y, GAO Y H, SUN X S. Effect of litter bed on growth performance and odor emission in fattening lamb. Scientia Agricultura Sinica, 2022, 55(24): 4943-4956. doi: 10.3864/j.issn.0578-1752.2022.24.012. (in Chinese)
[4]	高旭, 李建立, 路占云, 王孟雄, 郑忠, 刘从, 田树军. 羊床模式与环境气象因素对肉羊养殖异味气体水平的影响. 黑龙江畜牧兽医, 2025(2): 50-54.
	GAO X, LI J L, LU Z Y, WANG M X, ZHENG Z, LIU C, TIAN S J. Effects of sheep bed mode and environmental meteorological factors on odor gas levels in meat sheep farming. Heilongjiang Animal Science and Veterinary Medicine, 2025(2): 50-54. (in Chinese)
[5]	吴玉洪, 张世昌, 田茜, 马桂珍, 郭荣君, 李世东, 钟增明. 堆肥臭味物质: 粪臭素高效降解菌Rp3的分离和鉴定. 农业资源与环境学报, 2021, 38(4): 576-584.
	WU Y H, ZHANG S C, TIAN Q /X, MA G Z, GUO R J, LI S D, ZHONG Z M. Isolation and identification of a high-efficiency bacterial strain Rp3 to degrade skatole: an odor chemical in compost. Journal of Agricultural Resources and Environment, 2021, 38(4): 576-584. (in Chinese)
[6]	徐彦鹏, 吕道飞, 郭雅欣, 许锋, 颜健, 袁文兵, 陈忻. 三甲胺移除技术的研究进展. 广州化工, 2023, 51(11): 8-10.
	XU Y P, LÜ D F, GUO Y X, XU F, YAN J, YUAN W B, CHEN X. Research progress on removal technology of trimethylamine. Guangzhou Chemical Industry, 2023, 51(11): 8-10. (in Chinese)
[7]	韩鹏, 任爱玲, 郭斌, 律国黎. 过氧化氢改性活性炭对三甲胺废气的吸附. 河北科技大学学报, 2013, 34(2): 159-165.
	HAN P, REN A L, GUO B, LÜ G L. Hydrogen peroxide modified activated carbon for adsorption of trimethylamine exhaust. Journal of Hebei University of Science and Technology, 2013, 34(2): 159-165. (in Chinese)
[8]	杜国伟, 夏文水. 鲢鱼糜脱腥前后及贮藏过程中挥发性成分的变化. 食品工业科技, 2007, 28(9): 76-80.
	DU G W, XIA W S. The change of volatile compounds of ailver carp fish surimi before and after deodorization and during storage. Science and Technology of Food Industry, 2007, 28(9): 76-80. (in Chinese)
[9]	QIU Z L, LI G Y, AN T C. In vitro toxic synergistic effects of exogenous pollutants-trimethylamine and its metabolites on human respiratory tract cells. Science of the Total Environment, 2021, 783: 146915. doi: 10.1016/j.scitotenv.2021.146915
[10]	国家环境保护局, 国家技术监督局.. 恶臭污染物排放标准:GB14554—1993[S]. 北京: 中国标准出版社, 1994.
	State Bureau of Environmental Protection of the People’s Republic of China, State Bureau of Quality and Technical Supervision of the People’s Republic of China. EmissionSTANDARDsforodorpollutants:GB14554—1993[S]. Beijing: Standards Press of China, 1994. (in Chinese)
[11]	魏启航, 冯瑶, 王晓醒, 朱宏岗, 方昭, 李兆君. 除臭菌的筛选及其在堆肥中的应用效果. 中国农业科学, 2024, 57(13): 2623-2634. doi: 10.3864/j.issn.0578-1752.2024.13.010.
	WEI Q H, FENG Y, WANG X X, ZHU H G, FANG Z, LI Z J. Screening of deodorizing bacteria and its application in composting. Scientia Agricultura Sinica, 2024, 57(13): 2623-2634. doi: 10.3864/j.issn.0578-1752.2024.13.010. (in Chinese)
[12]	SETH M, MONDAL P, GHOSH D, BISWAS R, CHATTERJEE S, MUKHOPADHYAY S K. Metabolomic and genomic insights into TMA degradation by a novel halotolerant strain - Paracoccus sp. PS1. Archives of Microbiology, 2024, 206(4): 201. doi: 10.1007/s00203-024-03931-7
[13]	CHEN Y R, FANG S T, LIU H Y, ZHENG H M, HE Y, CHEN Z W, CHEN M X, ZHANG G X, ZHOU H W. Degradation of trimethylamine in vitro and in vivo by Enterococcus faecalis isolated from healthy human gut. International Biodeterioration & Biodegradation, 2018, 135: 24-32.
[14]	KIM S G, BAE H S, LEE S T. A novel denitrifying bacterial isolate that degrades trimethylamine both aerobically and anaerobically via two different pathways. Archives of Microbiology, 2001, 176(4): 271-277. doi: 10.1007/s002030100319
[15]	LIFFOURRENA A S, LUCCHESI G I. Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633). International Biodeterioration & Biodegradation, 2014, 90: 88-92.
[16]	陈薇, 刘标, 高帅帅, 唐冰璇, 李咏梅, 吴民熙, 尹红梅. 巨大芽孢杆菌Y2对猪粪堆肥腐熟度和氮素转化的影响. 家畜生态学报, 2024, 45(9): 79-84.
	CHEN W, LIU B, GAO S S, TANG B X, LI Y M, WU M X, YIN H M. Effects of Bacillus megatherium Y2 on maturity and nitrogen transformation during pig manure composting. Acta Ecologae Animalis Domastici, 2024, 45(9): 79-84. (in Chinese)
[17]	关健. 一株三甲胺降解菌的分离鉴定、生长和降解特性三甲胺对水域微生物影响的研究[D]. 杭州: 浙江大学, 2006.
	GUAN J. Isolation, identification, growth of a trimethylamine- degrading bacterium and effects of trimethylamine on microorganisms in water system[D]. Hangzhou: Zhejiang University, 2006. (in Chinese)
[18]	张生伟. 猪粪高效除臭菌株筛选及其除臭机理研究[D]. 兰州: 甘肃农业大学, 2016.
	ZHANG S W. Screening of efficient deodorant microbial strains and deodorizing mechanism of swine feces[D]. Lanzhou: Gansu Agricultural University, 2016. (in Chinese)
[19]	王艾伦, 金敬岗, 汪开英. 畜禽场微生物除臭技术的研究进展. 中国畜牧杂志, 2019, 55(1): 18-21, 28.
	WANG A L, JIN J G, WANG K Y. Research progress on microbial deodorization technologies in livestock farms. Chinese Journal of Animal Science, 2019, 55(1): 18-21, 28. (in Chinese)
[20]	王晓阁. 枯草芽孢杆菌研究进展与展望. 中山大学研究生学刊(自然科学医学版), 2012(3): 14-23.
	WANG X G. Research progress and prospect of Bacillus subtilis. Journal of the Graduates Sun Yat-Sen University (Natural Sciences, Medicine), 2012(3): 14-23. (in Chinese)
[21]	唐玉兰, 马悦, 何亚婷, 王晓华. 改性生物炭固定枯草芽孢杆菌对黑臭水体中污染物的处理效果研究. 安全与环境学报, 2023, 23(4): 1250-1260.
	TANG Y L, MA Y, HE Y T, WANG X H. Adsorption and degradation in the removal of pollutants in black and odorous water by Bacillus subtilis immobilized on biochar. Journal of Safety and Environment, 2023, 23(4): 1250-1260. (in Chinese)
[22]	BASTOS T S, DE LIMA D C, SOUZA C M M, MAIORKA A, DE OLIVEIRA S G, BITTENCOURT L C, FÉLIX A P. Bacillus subtilis and Bacillus licheniformis reduce faecal protein catabolites concentration and odour in dogs. BMC Veterinary Research, 2020, 16(1): 116. doi: 10.1186/s12917-020-02321-7
[23]	朱瑾, 朱红军. 枯草芽孢杆菌的作用机制及其在动物生产中的应用. 粮食与饲料工业, 2019(8): 47-51.
	ZHU J, ZHU H J. Mechanism of Bacillus subtilis and its application in animal production. Cereal & Feed Industry, 2019(8): 47-51. (in Chinese)
[24]	夏远舰, 杨小丽, 李海华, 赵博. 异养硝化-好氧反硝化菌Acinetobacter johnsonii sp. N26的脱氮性能及代谢途径. 微生物学通报, 2023, 50(4): 1374-1395.
	XIA Y J, YANG X L, LI H H, ZHAO B. Optimization of nitrogen removal performance and metabolic pathway of a heterotrophic nitrifying-aerobic denitrifying bacterial strain Acinetobacter johnsonii sp. N26. Microbiology China, 2023, 50(4): 1374-1395. (in Chinese)
[25]	王睿, 朱子恒, 舒文明, 余维初, 孙文秀. 一株不动杆菌JBX-006对聚丙烯酰胺的降解特性研究. 环境污染与防治, 2023, 45(10): 1369-1373, 1408.
	WANG R, ZHU Z H, SHU W M, YU W C, SUN W X. Research on the degradation of polyacrylamide by an Acinetobacter JBX-006. Environmental Pollution & Control, 2023, 45(10): 1369-1373, 1408. (in Chinese)
[26]	陈晓娟. 三甲胺高效降解菌Arthrobacter sp. TMA-1的分离鉴定及其降解特性研究[D]. 南京: 南京农业大学, 2011.
	CHEN X J. Isolation, identification and degradation characteristics of trimethylamine-degrading strain Arthur sp. TMA-1[D]. Nanjing: Nanjing Agricultural University, 2011. (in Chinese)
[27]	MATUSIAK K, OLEKSY M, BOROWSKI S, NOWAK A, KORCZYŃSKI M, DOBRZAŃSKI Z, GUTAROWSKA B. The use of Yucca schidigera and microbial preparation for poultry manure deodorization and hygienization. Journal of Environmental Management, 2016, 170: 50-59. doi: 10.1016/j.jenvman.2016.01.007
[28]	殷峻, 陈英旭, 刘和. 三甲胺降解细菌的分离、降解特性及其系统发育分析. 环境科学学报, 2004, 24(5): 883-889.
	YIN J, CHEN Y X, LIU H. Isolation, characterisation of biodegradation and phylogenetic analysis of trimethylamine-degrading bacteria. Acta Scientiae Circumstantiae, 2004, 24(5): 883-889. (in Chinese)
[29]	刘铜, 毕思宁, 史洁, 侯巨梅, 崔素萍, 王彦杰, 左豫虎, 于裴芝. 假单胞杆菌BS1培养条件的研究. 黑龙江八一农垦大学学报, 2013, 25(1): 7-11.
	LIU T, BI S N, SHI J, HOU J M, CUI S P, WANG Y J, ZUO Y H, YU P Z. Studies on optimal cultivation conditions of Pseudomonas sp. BS1. Journal of Heilongjiang August First Land Reclamation University, 2013, 25(1): 7-11. (in Chinese)
[30]	陈明霞, 施俊豪, 邢元军, 葛慧华. “环境因素对微生物生长的影响”的实验教学设计: 以粘质沙雷氏菌为例. 南阳师范学院学报, 2025, 24(1): 102-108.
	CHEN M X, SHI J H, XING Y J, GE H H. Experimental teaching design of“influence of environmental factors on the growth of microorganism”: A case study of Serratia marcescens. Journal of Nanyang Normal University, 2025, 24(1): 102-108. (in Chinese)
[31]	谢丹莹, 贾超祥, 杨晨, 张赛宇, 刘一, 李和平, 郭爽, 王月影. 1株蜡样芽孢杆菌噬菌体的分离与生物学特性分析. 黑龙江畜牧兽医, 2024(24): 71-75, 140.
	XIE D Y, JIA C X, YANG C, ZHANG S Y, LIU Y, LI H P, GUO S, WANG Y Y. Isolation and biological characterization analysis of one strain of bacteriophage of Bacillus cereus. Heilongjiang Animal Science and Veterinary Medicine, 2024(24): 71-75, 140. (in Chinese)
[32]	张璋, 赵腾飞, 李红霞, 宋露露. 产淀粉酶芽孢杆菌的筛选、耐酸性驯化及淀粉酶酶学性质分析. 中国酿造, 2024, 43(8): 74-79. doi: 10.11882/j.issn.0254-5071.2024.08.011
	ZHANG Z, ZHAO T F, LI H X, SONG L L. Screening and acid resistance acclimation of amylase-producing Bacillus and amylase characteristic analysis. China Brewing, 2024, 43(8): 74-79. (in Chinese) doi: 10.11882/j.issn.0254-5071.2024.08.011
[33]	PARWATA I P, OVIANTARI M V. Immobilization of lipase- producing bacteria Acinetobacter baumannii on paddy straw powder. Biosciences, Biotechnology Research Asia, 2016, 13(2): 661-668. doi: 10.13005/bbra/
[34]	高琳, 潘志华, 杨书运, 王立为, 徐慧, 董智强, 张婧婷, 黄蕾, 赵慧, 张君, 等. 碳源和巨大芽孢杆菌添加对土壤微生物环境及N₂O、CH₄排放的影响. 中国农业气象, 2016, 37(6): 645-653.
	GAO L, PAN Z H, YANG S Y, WANG L W, XU H, DONG Z Q, ZHANG J T, HUANG L, ZHAO H, ZHANG J, et al. Effects of carbon source and Bacillus megaterium on soil microbial environment and N₂O, CH₄ emission. Chinese Journal of Agrometeorology, 2016, 37(6): 645-653. (in Chinese)
[35]	许敏. 异源表达果聚糖蔗糖酶及其体外合成左聚糖的研究[D]. 天津: 天津大学, 2021.
	XU M. Heterologous expressed levansucrase and its study in levan synthesis in vitro[D]. Tianjin: Tianjin University, 2021. (in Chinese)
[36]	陈显玲, 宋连萍, 周燕妮, 王海芳, 谭夏云, 苏龙. 蔗糖磷酸化酶产生菌的筛选及其催化合成α-熊果苷条件优化. 中国酿造, 2022, 41(3): 117-124. doi: 10.11882/j.issn.0254-5071.2022.03.020
	CHEN X L, SONG L P, ZHOU Y N, WANG H F, TAN X Y, SU L. Screening of sucrose phosphorylase producing strain and enzymatic synthesis conditions optimization ofα-arbutin. China Brewing, 2022, 41(3): 117-124. (in Chinese)
[37]	王劲松, 关峰, 王学文, 许丽, 刘佐民, 张雷, 董玲. 蜡样芽孢杆菌和嗜酸乳杆菌发酵条件的优化. 饲料工业, 2022, 43(3): 16-20.
	WANG J S, GUAN F, WANG X W, XU L, LIU Z M, ZHANG L, DONG L. Fermentation optimization for high cell density culture of Bacillus cereus and Lactobacillus acidophilus. Feed Industry, 2022, 43(3): 16-20. (in Chinese)
[38]	OLEKSY-SOBCZAK M, KLEWICKA E. Optimization of media composition to maximize the yield of exopolysaccharides production by Lactobacillus rhamnosus strains. Probiotics and Antimicrobial Proteins, 2020, 12(2): 774-783. doi: 10.1007/s12602-019-09581-2
[39]	陈欣怡, 栗波, 赵杜娟, 汪博伦, 郭华楠, 付丽, 刘爱军, 杨辉, 王泽建. 不同碳源对E.adhaerens产维生素B12发酵代谢动力学分析. 华东理工大学学报(自然科学版), 2024, 50(1): 80-87.
	CHEN X Y, LI B, ZHAO D J, WANG B L, GUO H N, FU L, LIU A J, YANG H, WANG Z J. Kinetic analysis of fermentation metabolism of E.adhaerens producing vitamin B12 with different carbon sources. Journal of East China University of Science and Technology (Natural Science Edition), 2024, 50(1): 80-87. (in Chinese)
[40]	MINARI G D, PIAZZA R D, SASS D C, CONTIERO J. EPS production by Lacticaseibacillus casei using glycerol, glucose, and molasses as carbon sources. Microorganisms, 2024, 12(6): 1159. doi: 10.3390/microorganisms12061159
[41]	ZHANG J, WANG Q H, GONG Q L, GAO X. Symbiotic bacteria diversity and metabolome analyses of Nostoc sphaeroides grown under different organic carbon sources. Algal Research, 2024, 81: 103592. doi: 10.1016/j.algal.2024.103592
[42]	ZHANG M R, JIAO T, CHEN S G, ZHOU W Z. A review of microbial nitrogen transformations and microbiome engineering for biological nitrogen removal under salinity stress. Chemosphere, 2023, 341: 139949. doi: 10.1016/j.chemosphere.2023.139949
[43]	SANJAYA A P, PRASEPTIANGGA D, ZAMAN M Z, UMIATI V F, BARAJA S I. Effect of pH, temperature, and salt concentration on the growth of Bacillus subtilis T9-05 isolated from fish sauce. IOP Conference Series: Earth and Environmental Science, 2023, 1200(1): 012050.
[44]	陈红初. 高效柴油降解菌的筛选鉴定及其降解烷烃组分机理的研究[D]. 长春: 吉林大学, 2024.
	CHEN H C. Screening and identification of high-efficiency diesel degrading bacteria and the mechanism of degradation of alkane components[D]. Changchun: Jilin University, 2024. (in Chinese)
[45]	周杏荣, 周佳豪, 雷文平, 叶望娟, 周辉, 刘成国. 干酪乳杆菌LZ183E高密度培养条件优化. 中国酿造, 2020, 39(12): 64-68. doi: 10.11882/j.issn.0254-5071.2020.12.013
	ZHOU X R, ZHOU J H, LEI W P, YE W J, ZHOU H, LIU C G. Optimization of high density culture conditions of Lactobacillus casei LZ183E. China Brewing, 2020, 39(12): 64-68. (in Chinese)
[46]	王彩衣, 夏靖雯, 庞冰瑜, 覃丽媛, 程忠, 张婷. 高产纤维素酶菌株的筛选、固态发酵条件优化及其酶学性质研究. 中国酿造, 2024, 43(9): 105-111. doi: 10.11882/j.issn.0254-5071.2024.09.016
	WANG C Y, XIA J W, PANG B Y, QIN L Y, CHENG Z, ZHANG T. Screening and optimization of solid-state fermentation conditions of high cellulase-producing strain and its enzymatic property. China Brewing, 2024, 43(9): 105-111. (in Chinese) doi: 10.11882/j.issn.0254-5071.2024.09.016
[47]	丁佳乐, 韩先杰, 李树文. 酵母菌及其与芽孢杆菌复配去除水泡粪污水氨氮效果的研究. 畜牧与饲料科学, 2024, 45(6): 113-121.
	DING J L, HAN X J, LI S W. Research of ammonia nitrogen removal effects by yeasts and their combination with Bacillus spp. on effluent from pig manure cleaning by water submerging. Animal Husbandry and Feed Science, 2024, 45(6): 113-121. (in Chinese)
[48]	郭南南, 杨传伦, 张心青, 蔡倩倩, 周倩, 张萧萧, 傅英旬, 田杰伟, 马春峰. 苯胺降解菌的筛选、降解特性及其发酵优化. 微生物学通报, 2023, 50(3): 983-996.
	GUO N N, YANG C L, ZHANG X Q, CAI Q Q, ZHOU Q, ZHANG X X, FU Y X, TIAN J W, MA C F. Screening, degradation characteristics, and fermentation optimization of aniline-degrading strain. Microbiology China, 2023, 50(3): 983-996. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

菌株编号Strain number	菌名Bacterial name	覆盖率Coverage	同源性Homology	序列号Serial number
T2	枯草芽孢杆菌Bacillus subtilis	100%	100%	MT372156.1
T3	不动杆菌Acinetobacter sp.	100%	99.58%	MT757941.1

时间 Time (d)	CK组 CK group (mg·m^-3)	T2组 T2 group (mg·m^-3)	T3组 T3 group (mg·m^-3)
1	4.55±0.028aA	4.16±0.963aB	4.11±0.427aB
2	5.13±0.503aA	3.94±0.530abB	3.09±0.483bB
3	5.92±1.976aA	3.67±0.612abA	3.92±0.651abA
4	5.55±0.595aA	3.15±0.355bB	3.34±0.465abB
5	5.56±0.488aA	3.79±0.233abB	3.61±0.563abB
全期Overall	5.34±0.956A	3.74±0.494B	3.61±0.578B
处理Treatment	<0.01
时间Time	0.53
处理×时间 Treatment×Time	0.19

时间Time (d)	T2组 T2 group (%)	T3组 T3 group (%)
1	8.59±3.012bA	9.66±6.032aA
2	23.06±6.983abA	39.49±8.477aA
3	31.66±8.377abA	27.07±6.572aA
4	42.65±7.252aA	39.63±8.736aA
5	31.54±3.371abA	34.42±4.922aA
全期Overall	27.50±12.650A	30.05±12.490A
处理Treatment	0.64
时间Time	0.02
处理×时间 Treatment×Time	0.76

Isolation of Efficient Trimethylamine-Removing Strains and Optimization of Growth Conditions

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 12

References 48

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHONG RongYang, WEI ShouHui, WANG XiaoShan, SHI DuiHong, MIN LeiGuo, WANG WenHua, WANG JiangBo, XIAO XueMei. Aroma Formation and Key Volatile Compounds in Coriander Leaves Across Growth Stages Based on GC-MS and E-Nose [J]. Scientia Agricultura Sinica, 2026, 59(7): 1552-1563.
[2]	WU DongMing, XU Jing, YUAN JiaMei, WANG KeXuan, LI YuYi, HE Ping, ZHANG JianFeng, SONG DaLi, GAO Miao, ZHOU Wei. Isolation and Identification of Rhizosphere Growth-Promoting Bacteria of Myroides odoratimimus PJ-3 and Their Salt/ Alkali-Tolerance and Growth-Promoting Effects on Maize [J]. Scientia Agricultura Sinica, 2025, 58(20): 4131-4143.
[3]	LIU WenLong, CHANG YiFang, LI WenYu, SUN LiJuan, ZHENG ChangYing. Effects of Drought Stress During the Pupal Stage on Mating Behavior and Sensitivity to Lufenuron in Bradysia odoriphaga [J]. Scientia Agricultura Sinica, 2025, 58(14): 2793-2804.
[4]	WANG ZhiXiong, XU Dong, TIAN XiaoLi, WAN Peng, XIA Gen, SONG XuRong, WANG FuLian, GUI LianYou, ZHANG GuoHui. Cloning, Prokaryotic Expression and Ligand Binding Property of BminMinusOBP1 and BminPlusOBP1 from Bactrocera minax [J]. Scientia Agricultura Sinica, 2024, 57(24): 4894-4906.
[5]	DONG Qing, SONG LianJie, ZHANG HongWei, SU DongYao, ZHANG Ao, ZHANG Lu, ZHANG HuiWen, LI BoSen, GAO YuHong, SUN XinSheng. Isolation of High-Efficient Ammonia-Removing Strains and Its Cultivated Condition Optimization [J]. Scientia Agricultura Sinica, 2024, 57(21): 4367-4375.
[6]	WEI QiHang, FENG Yao, WANG XiaoXing, ZHU HongGang, FANG Zhao, LI ZhaoJun. Screening of Deodorizing Bacteria and Its Application in Composting [J]. Scientia Agricultura Sinica, 2024, 57(13): 2623-2634.
[7]	YANG Ling, TIAN XiaoLi, GUI LianYou, WANG FuLian, ZHANG GuoHui. Interaction Mechanisms Between Bactrocera minax Odorant-Binding Protein BminOBP6 and Its Ligands [J]. Scientia Agricultura Sinica, 2023, 56(7): 1311-1321.
[8]	LI ShaoHui, ZHAO Wei, LIU SongYan, LI PengLiang, ZHANG AiXia, LIU JingKe. Aroma Characteristics of Foxtail Millet Varieties from Different Ecological Regions by Analysis of SDE-GC-MS Combined with OPLS-DA [J]. Scientia Agricultura Sinica, 2023, 56(13): 2586-2596.
[9]	NIAN HeFen, ZHANG YuXi, LI BoLiao, CHEN XiuLin, LUO Kun, LI GuangWei. Expression and Ligand Binding Characteristics of GfunOBP2 from Grapholita funebrana [J]. Scientia Agricultura Sinica, 2023, 56(12): 2302-2316.
[10]	ZHAO HuiTing,PENG Zhu,JIANG YuSuo,ZHAO ShuGuo,HUANG Li,DU YaLi,GUO LiNa. Expression and Binding Properties of Odorant Binding Protein AcerOBP7 in Apis cerana cerana [J]. Scientia Agricultura Sinica, 2022, 55(3): 613-624.
[11]	CHE DaLu,ZHAO LiChen,CHENG SuCai,LIU AiYu,LI XiaoYu,ZHAO ShouPei,WANG JianCheng,WANG Yuan,GAO YuHong,SUN XinSheng. Effect of Litter Bed on Growth Performance and Odor Emission in Fattening Lamb [J]. Scientia Agricultura Sinica, 2022, 55(24): 4943-4956.
[12]	ZHANG YuanYuan,LIU WenJing,ZHANG BinBin,CAI ZhiXiang,SONG HongFeng,YU MingLiang,MA RuiJuan. Characterization of the Lactone Volatile Compounds in Different Types of Peach (Prunus persica L.) Fruit and Evaluations of Their Contributions to Fruit Overall Aroma [J]. Scientia Agricultura Sinica, 2022, 55(10): 2026-2037.
[13]	LI XiaoYing, WU JunKai, WANG HaiJing, LI MengYuan, SHEN YanHong, LIU JianZhen, ZHANG LiBin. Characterization of Volatiles Changes in Chinese Dwarf Cherry Fruit During Its Development [J]. Scientia Agricultura Sinica, 2021, 54(9): 1964-1980.
[14]	WANG Bing,LI HuiMin,CAO HaiQun,WANG GuiRong. Mechanisms and Applications of Plant-Herbivore-Natural Enemy Tritrophic Interactions Mediated by Volatile Organic Compounds [J]. Scientia Agricultura Sinica, 2021, 54(8): 1653-1672.
[15]	ZHAO WeiSong,GUO QingGang,DONG LiHong,WANG PeiPei,SU ZhenHe,ZHANG XiaoYun,LU XiuYun,LI SheZeng,MA Ping. Transcriptome and Proteome Analysis of Bacillus subtilis NCD-2 Response to L-proline from Cotton Root Exudates [J]. Scientia Agricultura Sinica, 2021, 54(21): 4585-4600.