绿肥联合麦秸还田对减量施氮春小麦光合特性和产量的影响

doi:10.3864/j.issn.0578-1752.2025.17.008

Abstract

Abstract:

【Objective】The aim of this study was to investigate the effects of green manure combined with wheat straw returning application on grain yield and leaf photosynthetic characteristics of spring wheat (Triticum aestivum L.) under different nitrogen reduction levels, which could provide theoretical basis and technical support for establishing spring wheat multiple cropping with green manure saving and efficient production pattern.【Method】The experiment was conducted at the Wuwei Oasis Agricultural Experimental Station of Gansu Agricultural University in 2022 and 2023. The split-plot designed experiment was adopted. The main plot was designed with different returning materials at three levels, including green manure and wheat straw combined returning application (W-GS), green manure returning application alone (W-G), and fallowing after wheat harvesting (W-F). The split plot was designed with four nitrogen application levels, including conventional nitrogen application rate (N1, 225 kg N·hm^-2), nitrogen application rate reduced about 15% (N2,190 kg N·hm^-2), about 30% (N3,155 kg N·hm^-2), and about 45% (N4, 120 kg N·hm^-2). The chlorophyll value (SPAD value), photosynthetic characteristics and grain yield of the top expending leaf of spring wheat was measured, and the leaf water use efficiency and light energy utilization efficiency was analyzed.【Result】Negative effect of nitrogen reduced application was observed on the grain yield of spring wheat, however, this effect was compensated even the grain yield of spring wheat was increased by green manure combined with wheat straw returning application. With the same nitrogen application rate, compared with that of W-F, the grain yield of spring wheat under W-GS and W-G increased by 15.3% and 9.4%, respectively, and the grain yield of spring wheat with W-GS was increased by 5.4% than that of W-G. The grain yield of spring wheat under W-F was decreased by the decreasing of nitrogen application rate, while that of W-GS had no significant difference between N3 and N1. The grain yield under W-GSN3 was not reduced compared with that under W-GSN1 and W-FN1. The photosynthetic characteristics of spring wheat leaf with nitrogen reduced application rate was improved by green manure combined with wheat straw returning application. The SPAD value, net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r) and leaf water use efficiency (LWUE) of spring wheat leaf under W-GS were significantly increased, respectively, compared with that of W-G and W-F under the same nitrogen application rate. With the decreasing of nitrogen application rate, the SPAD value, P_n, G_s, T_r and LWUE of spring wheat leaf under W-F was decreased significantly, however, there was no significant difference between N3 and N1 in W-GS. Comparison between combined treatments, there was no significant difference in photosynthetic characteristics of spring wheat under W-GSN3 compared with W-GSN1 and W-FN1. The light use efficiency (LUE) of spring wheat was significantly increased by 5.2% W-GS than that of W-F. The LUE of spring wheat with W-F decreased by the decreasing of nitrogen application rate, however, the LUE of spring wheat with W-GS was not significant difference between N3 and N1. There was no significant difference was observed in the LUE of spring wheat under W-GSN3 compared with that under W-GSN1 and W-FN1. The results of the path analysis indicated that, the grain yield of spring wheat was mainly determined by thousand kernel weight, which was affected by the regulation on net photosynthetic rate and transpiration rate via stomatal conductance of spring wheat leaf.【Conclusion】The green manure and wheat straw combined returning application mainly increased the thousand kernel weight of spring wheat by improving the photosynthetic characteristics of spring wheat leaf, and finally did not reduce the grain yield of spring wheat under 30% nitrogen reduced application rate. Therefore, the green manure and wheat straw combined returning application under nitrogen application rate of 155 kg·hm^-2 was recommended as a nitrogen saving and efficient production pattern of spring wheat multiple cropping with green manure in arid oasis irrigation region.

Key words: spring wheat, green manure, wheat straw returning application, nitrogen fertilizer reduced application, photosynthetic characteristics, yield

ZHANG HuanHuan, ZHANG DiaoLiang, WANG XiaoLi, CHEN Han, SHAO Juan, YIN Wen, HU FaLong, CHAI Qiang, FAN ZhiLong. Effects of Green Manure and Wheat Straw Combined Returning Application on Photosynthetic Characteristics and Yield of Spring Wheat Under Reduced Nitrogen Levels[J].Scientia Agricultura Sinica, 2025, 58(17): 3461-3472.

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References 36

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年份 Year	还田物料 Return materials	减氮水平 Nitrogen reduction levels	籽粒产量 Grain yield (kg·hm^-2)	产量构成因素Yield components
年份 Year	还田物料 Return materials	减氮水平 Nitrogen reduction levels	籽粒产量 Grain yield (kg·hm^-2)	单位面积穗数 Spike number (×10⁴·hm^-2)	穗粒数 Kernel number per spike	千粒重 Thousand-kernel weight (g)
2022	W-GS	N1	8513.6a	767.7a	37.1a	42.8a
		N2	8436.5ab	754.3ab	36.8a	42.6ab
		N3	8291.2abc	741.0b	36.2ab	41.7abc
		N4	7731.7d	680.7e	34.7cd	39.3d
	W-G	N1	8230.5bc	732.7cd	35.6b	41.3bc
		N2	8080.7c	731.3cd	35.4bc	41.1c
		N3	7548.6de	671.3ef	33.7e	38.1de
		N4	7333.2ef	653.3gh	33.2ef	37.1e
	W-F	N1	8038.5c	722.2d	35.3bc	41.0c
		N2	7027.1f	664.4fg	33.9de	37.5e
		N3	6707.8g	651.7h	32.7fg	35.6f
		N4	6363.9h	630.3i	32.2g	33.5g
2023	W-GS	N1	8584.9a	761.3a	37.3a	43.1a
		N2	8477.8ab	752.3ab	37.0a	42.9a
		N3	8315.9abc	743.3bc	36.4ab	42.0ab
		N4	7756.5d	683.0e	35.1cd	39.0c
	W-G	N1	8284.1bc	734.0cd	36.1b	41.9ab
		N2	8143.6c	732.3cd	35.8bc	41.4b
		N3	7688.2d	664.7f	33.8ef	38.3cd
		N4	7404.12e	651.4g	33.3fg	37.5d
	W-F	N1	8194.8c	727.7d	35.6bc	41.3b
		N2	7456.3e	670.3f	34.3de	37.7cd
		N3	6964.3f	650.3g	33.2fg	35.9e
		N4	6582.1g	631.7h	32.7g	33.6f
显著性(P值）Significance (P value)
还田物料Return materials, R			**	**	**	**
减氮水平Nitrogen reduction levels, N			**	**	**	**
还田物料×减氮水平R×N			**	**	*	**

Effects of Green Manure and Wheat Straw Combined Returning Application on Photosynthetic Characteristics and Yield of Spring Wheat Under Reduced Nitrogen Levels

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