Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (22): 4416-4430.doi: 10.3864/j.issn.0578-1752.2024.22.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Verification of SiCIPK21 Gene in Foxtail Millet

DU YanWei1(), YAN XiaoGuang1(), ZHAO JinFeng1(), JIA SuQing2, WANG GaoHong1, YU AiLi1, ZHANG Peng1   

  1. 1 Millet Research Institute, Shanxi Agricultural University, Changzhi 046011, Shanxi
    2 Maize Research Institute, Shanxi Agricultural University, Xinzhou 034000, Shanxi
  • Received:2024-05-12 Accepted:2024-07-12 Online:2024-11-16 Published:2024-11-22
  • Contact: YAN XiaoGuang, ZHAO JinFeng

RichHTML

22

PDF

586

Abstract:

【Objective】The Ca2+-CBL-CIPK signaling pathway has important functions in plant response to abiotic stresses. By cloning the SiCIPK21 gene and studying its function under stress conditions, we provide a key candidate gene and theoretical basis for molecular breeding of foxtail millet with stress tolerance.【Method】Bioinformatics was used to analyze the cis-acting elements in the promoter region of this gene and predict the interactions between this protein and AtCBLs in Arabidopsis thaliana. SiCIPK21 was cloned by PCR, and a fusion expression vector was constructed for transient expression in tobacco to determine the subcellular localization. foxtail millet cv. Yugu 1 was used as material, and specifically amplified part of the SiCIPK21 gene fragment from Yugu 1 leaves, and recombinant vector VIGS-pTRV2-SiCIPK21 was constructed, using the phytoene desaturase gene (SiPDS) as the indicator gene, and seedlings of foxtail millet at the two-leaf stage were selected and infiltrated by cotyledon injection to investigate the role of SiCIPK21 under salt stress (250 mmol·L-1 NaCl) by using virus-induced gene silencing (VIGS) technology. T3 generation transgenic lines were obtained by overexpressing SiCIPK21 in Arabidopsis thaliana. Phenotypes at germination were analyzed under different concentrations of NaCl (150/175 mmol·L-1), mannitol (300/400 mmol·L-1) and ABA (0.25/0.5 μmol·L-1) treatments, and salt and drought tolerant phenotypes at seedling stage were also analyzed.【Result】Subcellular localization revealed that SiCIPK21 was located in the nucleus. The protein SiCIPK21 might interact with AtCBL2, AtCBL3, AtCBL4, AtCBL9, and AtCBL10 in Arabidopsis thaliana. The promoter region of SiCIPK21 contained adverse response elements, suggesting that SiCIPK21 may participate in the adverse responses. The VIGS gene silencing demonstrated that SiCIPK21-silenced foxtail millet plants had increased sensitivity to salt stress than the control plants. Three independent T3 generation Arabidopsis thaliana overexpression lines (2#, 3# and 6#) were obtained by genetic transformation. Overexpression lines showed significantly higher germination rate, germination speed, green cotyledon unfolding rate, root length and fresh weight than the wild-type plants (WT) at different concentrations of NaCl (150/175 mmol·L-1), mannitol (300/400 mmol·L-1) and ABA (0.25/0.5 μmol·L-1). Moreover, phenotypic analysis of salt and drought tolerance in Arabidopsis seedlings showed that overexpression lines had significantly higher survival rates and chlorophyll contents than WT.【Conclusion】SiCIPK21 is a positive regulator of plant response to salt and drought stresses, which makes it a candidate gene for improving stress tolerance by molecular breeding in foxtail millet.

Key words: foxtail millet, SiCIPK21, abiotic stress, phenotype analysis, gene silencing

Table 1

Primer sequences used in this study"

引物名称 Primer name 引物序列 Primer sequence (5′-3′)
SiCIPK21-GFP-F ACTAGGGTCTCGCACCATGGCCATGGAGAAGAACCAAGACA
SiCIPK21-GFP-R ACTAGGGTCTCTCGCCCGTGGTGGTCCGCCTCG
VIGS-pTRV2-SiCIPK21-F AAGGTTACCGAATTCTCTAGGTTAGTGAAAAGGGGATCCTCATTTCCTC
VIGS-pTRV2-SiCIPK21-R GAGACGCGTGAGCTCGGTACGAAATTCTCCAGGTGATCATGGGTGTG
pCMBIA3301-SiCIPK21-F ACTAGGGTCTCGCACCATGGCCATGGAGAAGAACCAAGACA
pCMBIA3301-SiCIPK21-R ACTAGGGTCTCTACCGCTACGTGGTGGTCCGCCTC
RT-qPCR-SiCIPK21-F TAATCGCCAAGCACCTCCTA
RT-qPCR-SiCIPK21-R CTTGGTTCTTCTCCATGGCC
PCR-SiCIPK21-F ATGGCCATGGAGAAGAACCAAGACA
PCR-SiCIPK21-R CCTGTAGTTGCTCTGCGTGA
β-Actin-F CAGTGGACGCACAACAGGTAT
β-Actin-R AGCAAGGTCAAGACGGAGAAT

Fig. 1

Subcellular localization of SiCIPK21 in tobacco epidermis"

Table 2

Prediction of cis-elements in the promoter region of SiCIPK21 gene"

顺式元件Cis-element 典型序列Typical sequence 特性Characteristic
TGA-element AACGAC 生长素响应Auxin-responsive element
P-box CCTTTTG 赤霉素响应Gibberellin-responsive element
CGTCA-motif CGTCA 茉莉酸甲酯响应Cis-acting regulatory element involved in the MeJA-responsiveness
TGACG-motif TGACG
LTR CCGAAA 低温响应Cis-acting element involved in low-temperature responsiveness
MBS CAACTG 干旱响应MYB binding site involved in drought-inducibility
ARE AAACCA 厌氧响应Cis-acting regulatory element essential for the anaerobic induction
ATCT-motif AATCTAATCC 光响应Part of a conserved DNA module involved in light responsiveness
Box 4 ATTAAT
GATA-motif GATAGGA 光响应Part of a light responsive element
TCT-motif TCTTAC
chs-CMA2a TCACTTGA
AE-box AGAAACAA
MSA-like TCAAACGGT 细胞周期调控Cis-acting element involved in cell cycle regulation
TC-rich repeats ATTCTCTAAC 防御和应激反应响应Cis-acting element involved in defense and stress responsiveness

Fig. 2

Construction of VIGS vector of SiCIPK21 and characterization the salt tolerance of silencing SiCIPK21 in foxtail millet A: Construction of VIGS vector of SiCIPK21. a: PCR product amplification of SiCIPK21 fragment; b: Restriction digestion verification of pTRV2-SiCIPK21 vector. M: DNA Marker. B: Phenotypes of silenced SiPDS and SiCIPK21 in foxtail millet plants. a: Phenotype of control (pTRV2-00) and SiPDS-silenced plants (pTRV2-SiPDS) (The left is the control and the right is the SiPDS silent plant); b. Phenotypes of control (pTRV2-00) and SiCIPK21-silenced (pTRV2-SiCIPK21) plants under salt stress (250 mmol·L-1 NaCl) (The left is the control and the right is the SiCIPK21 silent plant); c: Phenotype of SiPDS-silenced plants. C: SiCIPK21 gene silencing efficiency detection. D: Chlorophyll content in leaves of control and SiCIPK21 silent plants under salt stress. E: MDA content in leaves of control and SiCIPK21 silent plants under salt stress. * and ** indicate significant difference at the 0.05 and 0.01 probability levels, respectively. The same as below"

Fig. 3

Identification of SiCIPK21 overexpression in Arabidopsis A: SiCIPK21 was detected by agarose gel electrophoresis; M: DNA marker DL2000. B: PCR detection of SiCIPK21 overexpression in Arabidopsis plants; 1, 2, 3, 6, 8, 10, 11, 12: Plants of SiCIPK21 overexpression, -: Negative control, +: Positive control. C: Relative expression levels of SiCIPK21 in Arabidopsis plants. *** indicates that the difference is extremely significant at the 0.001 probability levels"

Fig. 4

Phenotypes of Arabidopsis overexpressing SiCIPK21 under salt stress at germination stage A, B: Phenotype and opening cotyledon rate of overexpression Arabidopsis lines (2#, 3# and 6#) after treatment with 0, 150 and 175 mmol·L-1 NaCl for 12 d; C: Germination rate of overexpression Arabidopsis lines (2#, 3# and 6#) after treatment with 150 mmol·L-1 NaCl for 7 d; D-F: Phenotype, fresh weight and root length of overexpression Arabidopsis lines (2#, 3# and 6#) measured at 12 d after treatment with 0, 150 and 175 mmol·L-1 NaCl"

Fig. 5

Effect of seedling salt stress on the growth of SiCIPK21 overexpressing Arabidopsis A: Photographs of overexpression Arabidopsis lines (2#, 3# and 6#) before and after salt stress (200 mmol·L-1 NaCl); B: Statistics of survival rates; C: Chlorophyll content of overexpression Arabidopsis lines (2#, 3# and 6#) treated with NaCl"

Fig. 6

Osmotic tolerance of SiCIPK21 overexpressing Arabidopsis at germination stage A, B: Phenotype and opening cotyledon rate of overexpression Arabidopsis lines (2#, 3# and 6#) after treatment with 0, 300 and 400 mmol·L-1 mannitol for 12 d; C: Germination rate of overexpression Arabidopsis lines (2#, 3# and 6#) after treatment with 300 mmol·L-1 mannitol for 7 d; D-F: Phenotype, fresh weight and root length of overexpression Arabidopsis lines (2#, 3# and 6#) measured at 12 d after the treatment with 0, 300 and 400 mmol·L-1 mannitol"

Fig. 7

Effect of drought stress on the growth of seedlings overexpressing SiCIPK21 Arabidopsis A: Photographs of overexpression Arabidopsis lines (2#, 3# and 6#) before and after drought treatment; B: Statistics of survival rates; C: Chlorophyll content of overexpression Arabidopsis lines (2#, 3# and 6#) treated with drought"

Fig. 8

ABA tolerance in SiCIPK21 overexpressing Arabidopsis plants at germination stage A, B: Phenotype and opening cotyledon rate of overexpression Arabidopsis lines (2#, 3# and 6#) after treatment with ABA 0, 0.25 and 0.5 μmol·L-1 ABA for 12 d; C: Germination rate of overexpression Arabidopsis lines (2#, 3# and 6#) treatment with 0.25 μmol·L-1 ABA for 7 d; D-F: Phenotype, fresh weight and root length of overexpression Arabidopsis lines (2#, 3# and 6#) measured at 12 d after the treatment with 0, 0.25 and 0.5 μmol·L-1 ABA"

[1]
LI X K, GAO J H, SONG J Y, GUO K, HOU S Y, WANG X C, HE Q, ZHANG Y Y, ZHANG Y K, YANG Y L, TANG J Y, WANG H L, PERSSON S, HUANG M Q, XU L S, ZHONG L L, LI D Q, LIU Y M, WU H, DIAO X M, CHEN P, WANG X W, HAN Y H. Multi-omics analyses of 398 foxtail millet accessions reveal genomic regions associated with domestication, metabolite traits, and anti-inflammatory effects. Molecular Plant, 2022, 15(8): 1367-1383.
[2]
SANYAL S K, MAHIWAL S, NAMBIAR D M, PANDEY G K. CBL-CIPK module-mediated phosphoregulation: Facts and hypothesis. The Biochemical Journal, 2020, 477(5): 853-871.
[3]
DING X, LIU B W, SUN X Z, SUN X, ZHENG C S. New functions of CIPK gene family are continue to emerging. Molecular Biology Reports, 2022, 49(7): 6647-6658.

doi: 10.1007/s11033-022-07255-x pmid: 35229240
[4]
VERMA P, SANYAL S K, PANDEY G K. Ca2+-CBL-CIPK: A modulator system for efficient nutrient acquisition. Plant Cell Reports, 2021, 40(11): 2111-2122.
[5]
PATRA N, HARIHARAN S, GAIN H, MAITI M K, DAS A, BANERJEE J. TypiCal but DeliCate Ca++re: Dissecting the essence of calcium signaling network as a robust response coordinator of versatile abiotic and biotic stimuli in plants. Frontiers in Plant Science, 2021, 12: 752246.
[6]
ZENG H Q, ZHU Q Q, YUAN P G, YAN Y, YI K K, DU L Q. Calmodulin and calmodulin-like protein-mediated plant responses to biotic stresses. Plant, Cell & Environment, 2023, 46(12): 3680-3703.
[7]
KUDLA J, XU Q, HARTER K, GRUISSEM W, LUAN S. Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96(8): 4718-4723.
[8]
KOLUKISAOGLU U, WEINL S, BLAZEVIC D, BATISTIC O, KUDLA J. Calcium sensors and their interacting protein kinases: Genomics of the Arabidopsis and rice CBL-CIPK signaling networks. Plant Physiology, 2004, 134(1): 43-58.
[9]
DENG X M, HU W, WEI S Y, ZHOU S Y, ZHANG F, HAN J P, CHEN L H, LI Y, FENG J L, FANG B, LUO Q C, LI S S, LIU Y Y, YANG G X, HE G Y. TaCIPK29, a CBL-interacting protein kinase gene from wheat, confers salt stress tolerance in transgenic tobacco. PLoS ONE, 2013, 8(7): e69881.
[10]
CHEN X F, GU Z M, XIN D D, HAO L, LIU C J, HUANG J, MA B J, ZHANG H S. Identification and characterization of putative CIPK genes in maize. Journal of Genetics and Genomics, 2011, 38(2): 77-87.

doi: 10.1016/j.jcg.2011.01.005 pmid: 21356527
[11]
CUI Y P, SU Y, WANG J J, JIA B, WU M, PEI W F, ZHANG J F, YU J W. Genome-wide characterization and analysis of CIPK gene family in two cultivated allopolyploid cotton species: Sequence variation, association with seed oil content, and the role of GhCIPK6. International Journal of Molecular Sciences, 2020, 21(3): 863.
[12]
REN H M, ZHANG Y T, ZHONG M Y, HUSSIAN J, TANG Y T, LIU S K, QI G N. Calcium signaling-mediated transcriptional reprogramming during abiotic stress response in plants. Theoretical and Applied Genetics, 2023, 136(10): 210.
[13]
TRIPATHI V, PARASURAMAN B, LAXMI A, CHATTOPADHYAY D. CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plants. The Plant Journal, 2009, 58(5): 778-790.

doi: 10.1111/j.1365-313X.2009.03812.x pmid: 19187042
[14]
陈小晶, 王东梅, 关红辉, 郭剑, 沙小茜, 李永祥, 张登峰, 刘旭洋, 何冠华, 石云素, 宋燕春, 王天宇, 黎裕, 刘颖慧, 李春辉. 玉米CIPK基因家族的鉴定及ZmCIPK3的抗旱性功能研究. 植物遗传资源学报, 2022, 23(4): 1064-1075.

doi: 10.13430/j.cnki.jpgr.20220107006
CHEN X J, WANG D M, GUAN H H, GUO J, SHA X Q, LI Y X, ZHANG D F, LIU X Y, HE G H, SHI Y S, SONG Y C, WANG T Y, LI Y, LIU Y H, LI C H. Identification of CIPK gene family members and investigation of the drought tolerance of ZmCIPK3 in maize. Journal of Plant Genetic Resources, 2022, 23(4): 1064-1075. (in Chinese)
[15]
XIANG Y, HUANG Y M, XIONG L Z. Characterization of stress-responsive CIPK genes in rice for stress tolerance improvement. Plant Physiology, 2007, 144(3): 1416-1428.

doi: 10.1104/pp.107.101295 pmid: 17535819
[16]
RÓDENAS R, VERT G. Regulation of root nutrient transporters by CIPK23: 'One kinase to rule them all'. Plant & Cell Physiology, 2021, 62(4): 553-563.
[17]
ZHANG Y, ZHOU X N, LIU S Y, YU A Z, YANG C M, CHEN X L, LIU J Y, WANG A X. Identification and functional analysis of tomato CIPK gene family. International Journal of Molecular Sciences, 2019, 21(1): 110.
[18]
PODDAR N, DEEPIKA D, CHITKARA P, SINGH A, KUMAR S. Molecular and expression analysis indicate the role of CBL interacting protein kinases (CIPKs) in abiotic stress signaling and development in chickpea. Scientific Reports, 2022, 12(1): 16862.
[19]
LU L, WU X R, TANG Y, ZHU L M, HAO Z D, ZHANG J B, LI X L, SHI J S, CHEN J H, CHENG T L. Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress. Frontiers in Plant Science, 2022, 13: 1052463.
[20]
YANG W Q, KONG Z S, OMO-IKERODAH E, XU W Y, LI Q, XUE Y B. Calcineurin B-like interacting protein kinase OsCIPK23 functions in pollination and drought stress responses in rice (Oryza sativa L.). Journal of Genetics and Genomics, 2008, 35(9): 531-543.
[21]
ZHAO J F, YU A L, DU Y W, WANG G H, LI Y F, ZHAO G Y, WANG X D, ZHANG W Z, CHENG K, LIU X, WANG Z H, WANG Y W. Foxtail millet (Setaria italica (L.) P. Beauv) CIPKs are responsive to ABA and abiotic stresses. PLoS ONE, 2019, 14(11): e0225091.
[22]
高俊凤. 植物生理学实验指导. 北京: 高等教育出版社, 2006.
GAO J F. Laboratory Instruction of Plant Physiology. Beijing: Higher Education Press, 2006. (in Chinese)
[23]
KANWAR P, SANYAL S K, TOKAS I, YADAV A K, PANDEY A, KAPOOR S, PANDEY G K. Comprehensive structural, interaction and expression analysis of CBL and CIPK complement during abiotic stresses and development in rice. Cell Calcium, 2014, 56(2): 81-95.

doi: 10.1016/j.ceca.2014.05.003 pmid: 24970010
[24]
ZHANG H F, YANG B, LIU W Z, LI H W, WANG L, WANG B Y, DENG M, LIANG W W, DEYHOLOS M K, JIANG Y Q. Identification and characterization of CBL and CIPK gene families in canola (Brassica napus L.). BMC Plant Biology, 2014, 14: 8.
[25]
LI J, JIANG M M, REN L, LIU Y, CHEN H Y. Identification and characterization of CBL and CIPK gene families in eggplant (Solanum melongena L.). Molecular Genetics and Genomics, 2016, 291(4): 1769-1781.
[26]
SUN T, WANG Y, WANG M, LI T T, ZHOU Y, WANG X T, WEI S Y, HE G Y, YANG G X. Identification and comprehensive analyses of the CBL and CIPK gene families in wheat (Triticum aestivum L.). BMC Plant Biology, 2015, 15: 269.
[27]
SHINOZAKI K, YAMAGUCHI-SHINOZAKI K. Molecular responses to dehydration and low temperature: Differences and cross-talk between two stress signaling pathways. Current Opinion in Plant Biology, 2000, 3(3): 217-223.

pmid: 10837265
[28]
BASU S, ROYCHOUDHURY A, SENGUPTA D N. Deciphering the role of various cis-acting regulatory elements in controlling SamDC gene expression in rice. Plant Signaling & Behavior, 2014, 9(1): e28391.
[29]
KIM K N, CHEONG Y H, GRANT J J, PANDEY G K, LUAN S. CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. The Plant Cell, 2003, 15(2): 411-423.
[30]
GAZZARRINI S, MCCOURT P. Genetic interactions between ABA, ethylene and sugar signaling pathways. Current Opinion in Plant Biology, 2001, 4(5): 387-391.

pmid: 11597495
[31]
SPOLLEN W G, LENOBLE M E, SAMUELS T D, BERNSTEIN N, SHARP R E. Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiology, 2000, 122(3): 967-976.

pmid: 10712561
[32]
MAO J J, MANIK S M, SHI S J, CHAO J T, JIN Y R, WANG Q, LIU H B. Mechanisms and physiological roles of the CBL-CIPK networking system in Arabidopsis thaliana. Genes, 2016, 7(9): 62.
[33]
YADAV A K, JHA S K, SANYAL S K, LUAN S, PANDEY G K. Arabidopsis calcineurin B-like proteins differentially regulate phosphorylation activity of CBL-interacting protein kinase 9. The Biochemical Journal, 2018, 475(16): 2621-2636.
[34]
YU Q Y, AN L J, LI W L. The CBL-CIPK network mediates different signaling pathways in plants. Plant Cell Reports, 2014, 33(2): 203-214.

doi: 10.1007/s00299-013-1507-1 pmid: 24097244
[35]
ZHU J K. Abiotic stress signaling and responses in plants. Cell, 2016, 167(2): 313-324.
[36]
KAYA C, UĞURLAR F, ADAMAKIS I D S. Molecular mechanisms of CBL-CIPK signaling pathway in plant abiotic stress tolerance and hormone crosstalk. International Journal of Molecular Sciences, 2024, 25(9): 5043.
[37]
HASEGAWA P M, BRESSAN R A, ZHU J K, BOHNERT H J. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology, 2000, 51: 463-499.

pmid: 15012199
[38]
ZHU J K. Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 2002, 53: 247-273.
[39]
CUI X Y, DU Y T, FU J D, YU T F, WANG C T, CHEN M, CHEN J, MA Y Z, XU Z S. Wheat CBL-interacting protein kinase 23 positively regulates drought stress and ABA responses. BMC Plant Biology, 2018, 18(1): 93.
[40]
HUSSAIN Q, ASIM M, ZHANG R, KHAN R, FAROOQ S, WU J S. Transcription factors interact with ABA through gene expression and signaling pathways to mitigate drought and salinity stress. Biomolecules, 2021, 11(8): 1159.
[41]
SCHACHTMAN D P, GOODGER J Q D. Chemical root to shoot signaling under drought. Trends in Plant Science, 2008, 13(6): 281-287.

doi: 10.1016/j.tplants.2008.04.003 pmid: 18467158
[42]
YAMAGUCHI-SHINOZAKI K, SHINOZAKI K. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology, 2006, 57: 781-803.
[43]
QIN F, SHINOZAKI K, YAMAGUCHI-SHINOZAKI K. Achievements and challenges in understanding plant abiotic stress responses and tolerance. Plant and Cell Physiology, 2011, 52(9): 1569-1582.

doi: 10.1093/pcp/pcr106 pmid: 21828105
[44]
PIAO H L, XUAN Y H, PARK S H, JE B I, PARK S J, PARK S H, KIM C M, HUANG J, WANG G K, KIM M J, KANG S M, LEE I J, KWON T R, KIM Y H, YEO U S, YI G, SON D, HAN C D. OsCIPK31, a CBL-interacting protein kinase is involved in germination and seedling growth under abiotic stress conditions in rice plants. Molecules and Cells, 2010, 30(1): 19-27.
[1] ZHOU HaoLu, SHEN ZhaoYang, LUO XinYu, HUANG YingHui, WANG KeXin, WANG YunHao, GAO XiaoLi. The Effect of Nitrogen Fertilizer on Nitrogen Use Efficiency and Yield of Foxtail Millet in Ridge-Furrow Rainwater Harvesting Planting Model [J]. Scientia Agricultura Sinica, 2024, 57(5): 885-899.
[2] LI ChuXin, SONG ChenHu, ZHOU JinHuan, LI JiaXin, WANG XinLiang, TIAN XuBin, SONG Zhen. Research on Prevention and Control Technology of Citrus Yellow Vein Clearing Virus Based on VIGS [J]. Scientia Agricultura Sinica, 2024, 57(22): 4473-4482.
[3] LI Jie, LIANG ZhiLin, SUN Yan, TAN GenJia, HUAI BaoYu. Functional Analysis of SlSnRK1.2 in Regulating Tomato Resistance to Grey Mould [J]. Scientia Agricultura Sinica, 2024, 57(21): 4238-4247.
[4] DONG ErWei, WANG Yuan, WANG JinSong, LIU QiuXia, HUANG XiaoLei, JIAO XiaoYan. Effects of Nitrogen Fertilization Levels on Grain Yield, Plant Nitrogen Utilization Characteristics and Grain Quality of Foxtail Millet [J]. Scientia Agricultura Sinica, 2024, 57(2): 306-318.
[5] LIU DeLong, LI ShiRu, WANG ChuanXing, GUO ShuQing, MA ZhiXiu, WU YongJiang, HAN HuiBing, LI YuJie, ZHANG PanPan, YANG Pu. Phenotypical Variation and Dynamic QTL Mapping of Plant Height in Foxtail Millet at Different Developmental Stages [J]. Scientia Agricultura Sinica, 2024, 57(18): 3533-3550.
[6] LOU Hui, ZHU JinCheng, HAN ZeGang, ZHANG Wei. Identification and Functional Analysis of the 5-Oxoprolinase Genes in Fusarium oxysporum [J]. Scientia Agricultura Sinica, 2024, 57(10): 1915-1929.
[7] LI Cheng, LU Kai, WANG CaiLin, ZHANG YaDong. Research Progress of PPR Protein in Plant Abiotic Stress Response [J]. Scientia Agricultura Sinica, 2023, 56(24): 4801-4813.
[8] DONG YanYu, XU BiYu, DONG ZeYu, WANG LuYao, CHEN JinWen, FANG Lei. Genome-Wide Identification and Interspecific Comparative Analysis of the EXO70 Gene Family in Cotton [J]. Scientia Agricultura Sinica, 2023, 56(23): 4621-4634.
[9] QIN Na, FU SenJie, ZHU CanCan, DAI ShuTao, SONG YingHui, WEI Xin, WANG ChunYi, YE ZhenYan, LI JunXia. QTL Analysis for Seeding Traits Related to Low Nitrogen Tolerance in Foxtail Millet [J]. Scientia Agricultura Sinica, 2023, 56(20): 3931-3945.
[10] ZHANG YuJia, CUI KaiWen, DUAN LiSheng, CAO AiPing, XIE QuanLiang, SHEN HaiTao, WANG Fei, LI HongBin. Identification and Expression of CAD and CAD-Like Gene Families from Gossypium barbadense and Their Response to Verticillium dahliae [J]. Scientia Agricultura Sinica, 2023, 56(19): 3759-3771.
[11] LI ShaoHui, ZHAO Wei, LIU SongYan, LI PengLiang, ZHANG AiXia, LIU JingKe. Aroma Characteristics of Foxtail Millet Varieties from Different Ecological Regions by Analysis of SDE-GC-MS Combined with OPLS-DA [J]. Scientia Agricultura Sinica, 2023, 56(13): 2586-2596.
[12] MO WenJing,ZHU JiaWei,HE XinHua,YU HaiXia,JIANG HaiLing,QIN LiuFei,ZHANG YiLi,LI YuZe,LUO Cong. Functional Analysis of MiZAT10A and MiZAT10B Genes in Mango [J]. Scientia Agricultura Sinica, 2023, 56(1): 193-202.
[13] DONG YongXin,WEI QiWei,HONG Hao,HUANG Ying,ZHAO YanXiao,FENG MingFeng,DOU DaoLong,XU Yi,TAO XiaoRong. Establishment of ALSV-Induced Gene Silencing in Chinese Soybean Cultivars [J]. Scientia Agricultura Sinica, 2022, 55(9): 1710-1722.
[14] JIA GuanQing, DIAO XianMin. Current Status and Perspectives of Innovation Studies Related to Foxtail Millet Seed Industry in China [J]. Scientia Agricultura Sinica, 2022, 55(4): 653-665.
[15] SU Qian,DU WenXuan,MA Lin,XIA YaYing,LI Xue,QI Zhi,PANG YongZhen. Cloning and Functional Analyses of MsCIPK2 in Medicago sativa [J]. Scientia Agricultura Sinica, 2022, 55(19): 3697-3709.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd