Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (10): 1948-1959.doi: 10.3864/j.issn.0578-1752.2018.10.014

• HORTICULTURE • Previous Articles     Next Articles

Identification and Expression Analysis Under Abiotic Stresses of OFP Gene Family in Apple

RuiRui XU1,2(), Rui LI2, XiaoFei WANG2, YuJin HAO2   

  1. 1College of Biological and Agricultural Engineering, Weifang University/Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong, Weifang 261061, Shandong
    2College of Horticulture Science and Technology, Shandong Agricultural University/National Research Center for Apple Engineering and Technology, Tai’an 271018, Shandong;
  • Received:2017-09-20 Accepted:2017-12-07 Online:2018-05-16 Published:2018-05-16

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Abstract:

【Objective】 Identification of the OFP (OVATE family protein) genes from apple genome and analysis of gene characteristic, tissue expression pattern and response to abiotic stresses of OFP family genes in apple will be useful to the functional analysis of plant OFP genes. 【Method】 Based on apple genome database, the OFP gene family members were identified and the genes were analyzed using bioinformatics methods. A phylogenetic tree was created using the MEGA5.0 program. Gene structure and chromosomes location were carried out by MapDraw and GSDS separately. Expression pattern analysis of OFP genes in different tissues was done based on the existing microarray database and qRT-PCR. The expression of 13 MdOFPs genes was also analyzed under various stress conditions using qRT-PCR. 【Result】 A total of 28 OFP genes was systematically identified from apple genome and classified into 4 groups including 13, 6, 4 and 5 members according to the gene structure and conserved domain phylogeny relationship. All OFP genes are distributed on 13 apple chromosomes with the largest number six MdOFP on Chr12, suggesting that they have an extensive distribution on the apple chromosomes. Most of the OFP genes have distinctive expression patterns in tissues and response to NaCl and PEG treatment stresses in root and shoot, respectively. MdOFP04 and MdOFP20 were up-regulated obviously in root and shoot, while MdOFP01, MdOFP12 and MdOFP18 have opposite expression pattern in root and shoot under NaCl stress. Temperature stresses significantly regulate the expression of MdOFP and the expression of MdOFP04 and MdOFP17 were significantly increased after high temperature and low temperature stresses. 【Conclusion】 Twenty-eight OFP genes in apple were identified by genome-wide screening. They are classified into four groups and distributed on 13 chromosomes with different tissue patterns and different stress response patterns. These results will be helpful to the functional analysis of OFP genes in apple.

Key words: apple, OFP gene family, phylogeny analysis, stress, gene expression

Table 1

The primers used in the qRT-PCR"

基因名称 Gene name 上游引物（5′-3′） Forward primer (5′-3′) 下游引物（5′-3′） Reverse primer (5′-3′)
MdOFP01 TCCCGTTTAGTTCTTCATCTTTC CAGTGTGTCAATCTCGTCATC
MdOFP03 CGTGGTAGAAAGAGTGTGTC GATAGCAAGCAAGCAGGTC
MdOFP04 CAACAACAACTCGTCCTTACAG TTCCTTCACCACCGCAATG
MdOFP06 CGGAGGATGGGTTGGATTC TTCTTGTTCTTCTTCAGTTCGC
MdOFP07 CGGAGGATGGGTTGGATTC TTCTTGTTCTTCTTCAGTTCGC
MdOFP08 CAATGGAGGAAATGGTGGAATG AGCAGAAAGAGCAAGAAGGAG
MdOFP11 GGAGGAGAAGACGCAATAGAG CCCATCACCACCATCATCAG
MdOFP12 TTCAAGCCGTTCAGCCTCAG GTTCATCACAAGACCGCCATC
MdOFP13 AGAGAGAAACACCACCGAAG TTGCCCTGAGCGAGAAAG
MdOFP16 CTCCTTCTTGCTCTTTCTGC CATCCTCCTCCTGACCTTG
MdOFP17 AGGGTGAATGGTAAGAGCAATC GACGACAGCGACGAAGAAG
MdOFP18 TGCTGTGGTGAAGAAGTCG CCCTGTGATGGTGTGTCG
MdOFP20 TCCTCCTCGCAGTCACAC TTCCTTCACCACCGCAATG
Md18S ACACGGGGAGGTAGTGACAA CCTCCAATGGATCCTCGTTA

Table 2

The Ovate proteins gene family in apple"

基因名称Gene name 基因ID号
Gene Identifier		 染色体定位
Chromosome location		 编码序列长度
CDS length
(bp)		 外显子
数目
Exon no.		 大小
Size
(aa)		 分子量Molecular weight (Da) 等电点Isoelectric Point 拟南芥同源基因
Best homologs in Arabidopsis
MdOFP01 MDP0000243940 chr2:4251394..4252275 882 1 293 33533.3 9.13 AT5G66270.1
MdOFP02 MDP0000155311 chr2:13187873..13188835 963 1 320 25246.9 9.10 AT1G19860.1
MdOFP03 MDP0000684645 chr3:4952419..4953570 1152 1 383 41697.8 8.99 AT1G19860.1
MdOFP04 MDP0000141642 chr3:16219260..16220244 675 2 224 25723.6 9.51 AT1G19860.1
MdOFP05 MDP0000733399 chr3:31034178..31034924 747 1 248 79142.9 5.58 AT2G02160.1
MdOFP06 MDP0000134728 chr4:17309604..17310695 1092 1 363 72112.4 6.00 AT5G12440.3
MdOFP07 MDP0000147045 chr4:17310063..17310721 627 2 208 26669.0 8.73 AT3G12130.1
MdOFP08 MDP0000158931 chr5:941980..942486 507 1 168 51797.0 8.27 AT5G18550.1
MdOFP09 MDP0000241313 chr5:5755844..5757320 1296 2 431 30624.6 9.44 AT3G12130.1
MdOFP10 MDP0000402356 chr7:5438947..5439702 756 1 251 40999.8 8.81 AT3G08505
MdOFP11 MDP0000296199 chr8:3579420..3580445 1026 1 341 47229.3 7.10 AT2G41900.1
MdOFP12 MDP0000155705 chr10:26964000..26965493 1296 2 431 79656.8 6.14 AT3G51950
MdOFP13 MDP0000909475 chr10:32149450..32149956 507 1 168 16845.6 8.27 AT5G26749.1
MdOFP14 MDP0000156202 chr11:4775252..4776394 1143 1 380 91640.3 7.22 AT1G10320.1
MdOFP15 MDP0000311969 chr11:22625025..22626007 549 2 182 75934.3 6.24 AT1G30460.1
MdOFP16 MDP0000127146 chr11:32348926..32350337 912 2 303 73772.2 6.10 AT1G30460.1
MdOFP17 MDP0000669940 chr12:3242911..3248219 957 3 318 20954.5 6.24 AT3G48440.1
MdOFP18 MDP0000137053 chr12:3317131..3317688 558 1 185 65503.5 9.21 AT1G19860.1
MdOFP19 MDP0000456557 chr12:14175298..14181532 1317 3 438 98062.9 5.78 AT3G51120.1
MdOFP20 MDP0000692068 chr12:21920389..21922396 1098 2 365 20469.1 9.34 AT1G66810.1
MdOFP21 MDP0000238712 chr12:21920806..21921922 1065 2 354 93378.1 8.34 AT3G27700
MdOFP22 MDP0000332435 chr12:25821429..25822584 1161 1 386 77290.9 6.31 AT2G33835.1
MdOFP23 MDP0000410437 chr13:18948503..18949252 750 1 249 76306.6 6.34 AT2G41900.1
MdOFP24 MDP0000943578 chr13:25670394..25671158 765 1 254 57322.6 5.28 AT1G01350.1
MdOFP25 MDP0000243386 chr14:4693458..4695714 1020 2 339 22465.2 6.99 AT3G19360.1
MdOFP26 MDP0000140421 chr15:11737514..11738332 822 1 273 44526 9.32 AT3G47120.1
MdOFP27 MDP0000259366 chr17:16535235..16536098 864 1 287 76587.0 5.93 AT2G02160.1
MdOFP28 MDP0000921224 chr0:61416319..61416894 576 1 191 172448.5 8.12 AT1G21580.1

Fig. 1

The unrooted neighbor joining phylogenetic tree and gene structure of OFP gene family in apple"

Fig. 2

The chromosome location of the OFP gene family in apple"

Fig. 3

Synteny analysis of OFP genes between apple and Arabidopsis"

Fig. 4

Expression profile of MdOFP in different tissues by microarray analysis"

Fig. 5

Expression profile of MdOFP in different tissues in apple"

Fig. 6

Expression analysis of the MdOFP under NaCl and PEG treatment in apple"

Fig. 7

Expression analysis of the MdOFP under high temperature (HT) and low temperature (LT) treatment in apple"

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