Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (23): 4698-4706.doi: 10.3864/j.issn.0578-1752.2015.23.011

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Mineralization Characteristics of Upland Soil Labile and Recalcitrant Nitrogen Under Long-Term Different Fertilization Systems

WU Hong-liang1, YU Wei-shui1, ZHU Ping2, ZHANG Shui-qing3, ZHAO Ya-wen1, LIU Jing1,  WANG Shi-chao 1, MENG Fan-hua4, LU Chang-ai1   

  1. 1Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable Land, Beijing 100081
    2Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, Changchun 130124
    3Institute of Plant Nutrition and Environmental Resources, Henan Academy of Agricultural Sciences, Zhengzhou 450002
    4Henan Soil and Fertilizer Station, Zhengzhou 450002
  • Received:2015-09-16 Online:2015-12-01 Published:2015-12-01

RichHTML

5

PDF

487

Abstract: 【Objective】Labile nitrogen (Lab-N) and recalcitrant nitrogen (Rec-N) are important components of soil organic matter, and their mineralization ability has an effect on organic nitrogen turnover properties. The objective of this study is to research the mineralization characteristics of soil labile and recalcitrant nitrogen under different long-term fertilization, and to provide a basis for different fertilization measures and their nitrogen supplying capacities. 【Method】Using laboratory incubation method, soil labile and recalcitrant nitrogen fractions separated by particle size-density separation method were studied under four treatments, namely no fertilizer (CK), chemical fertilizer (NPK), chemical fertilizer combined with straw (NPKS), and chemical fertilizer combined with manure (NPKM) from two typical long-term experiment sites (black soil and fluvo-aquic soil) in China. 【Result】Results of laboratory sieving and incubation showed that the method was simple and suitable to study the mineralization characteristics of soil labile and recalcitrant nitrogen fractions in which average soil mass recovery and average soil nitrogen recovery were both above 97%, and average mineralization contribution rate (soil labile and recalcitrant nitrogen fractions mineralization amount to that of original soil) was 99.91% (99.89%-99.93%). Net-nitrogen (Net-N) mineralization potential of soil labile nitrogen under treatments of NPK, NPKS and NPKM were higher than that under CK treatments by 26.82%-137.10% (except NPK of black soil). Different fertilizer treatments had a significant influence on Net-N mineralization potential of soil labile nitrogen in black soil and fluvo-aquic soil. The Net-N mineralization potential of soil labile nitrogen in black soil under NPKM treatment was 1.48 mg?kg-1?d-1, which was higher compared with NPKS (1.02 mg?kg-1?d-1) and NPK (0.75 mg?kg-1?d-1). The Net-N mineralization potential of soil labile nitrogen in fluvo-aquic soil under NPKM treatment was 1.17 mg?kg-1?d-1, which was higher compared with NPKS (0.89 mg?kg-1?d-1) and NPK (0.76 mg?kg-1?d-1). The Net-N mineralization potential of soil recalcitrant nitrogen had no significant difference among different fertilizer treatments. The average mineralization potential was 0.58 mg?kg-1?d-1 (0.52-0.63 mg?kg-1?d-1) in black soil and 0.51 mg?kg-1?d-1 (0.40-0.62 mg?kg-1?d-1) in fluvo-aquic soil. The Net-N mineralization potential of soil labile nitrogen was higher than that of soil recalcitrant nitrogen in both black soil and fluvo-aquic soil, and it showed the biggest difference under the NPKM treatment. Among them, the Net-N mineralization potential of soil labile nitrogen was 1.41, 1.39, 1.75, and 2.35 times that of soil recalcitrant nitrogen as the order of CK, NPK, NPKS, and NPKM in black soil, and was 1.22, 1.33, 1.56, and 1.87 times as the same order in fluvo-aquic soil. The mineralization contribution rate of soil labile nitrogen was influenced by different fertilizer measures in this order CK, NPKsignificantly higher than the other treatments. There were no significant differences among the mineralization contribution rate of upland recalcitrant nitrogen under different fertilizer treatments. The average mineralization contribution rates were 86.24% (83.96%-88.51%) in black soil and 88.46% (86.71%-90.20%) in fluvo-aquic soil. 【Conclusion】 The Net-N mineralization potential and mineralization contribution rates of upland soil labile nitrogen were more sensitive to different fertilizer measures than that in upland soil recalcitrant nitrogen. Under different fertilization measures, the Net-N mineralization potential of soil labile nitrogen was significantly higher than that of soil recalcitrant nitrogen, especially for the treatment of NPKM. Long-term application of NPKS or NPKM will improve Net-N mineralization potential and mineralization contribution rate of upland soil. The improvement effect is in the order of NPKM＞NPKS＞NPK.

Key words:  liable nitrogen, recalcitrant nitrogen, black soil, fluvo-aquic soil, characteristics of mineralization; long-term fertilization system

[1]    胡田田, 李生秀, 郝乾坤. 旱地土壤矿质氮和可矿化氮与土壤供氮能力的关系. 水土保持学报, 2000, 14(4): 83-86, 103.
Hu T T, Li S X, Hao Q K. Relationship between soil mineralized N, mineralizable N and soil nitrogen-supplying capacity. Journal of Soil and Water Conservation, 2000, 14(4): 83-86,103. (in Chinese)
[2]    李生秀. 中国旱地土壤植物氮素. 北京: 科学出版社, 2008.
Li S X. Plants Nitrogen of Upland Soil in China. Beijing: Science Press, 2008. (in Chinese)
[3]    王媛, 周建斌, 杨学云. 长期不同培肥处理对土壤有机氮组分及氮素矿化特性的影响. 中国农业科学, 2010, 43(6): 1173-1180.
Wang Y, Zhou J B, Yang X Y. Effects of different long-term fertilization on the fractions of organic nitrogen and nitrogen mineralization in soils. Scientia Agricultura Sinica, 2010, 43(6): 1173-1180. (in Chinese)
[4]    李世清, 沈玉芳. 黄土高原土壤有机氮及其矿化. 北京: 科学出版社, 2010.
Li S Q, Shen Y F. Soil Organic Nitrogen and Mineralization of the Loess Plateau in China. Beijing: Science Press, 2010. (in Chinese)
[5]    田茂洁. 土壤氮素矿化影响因子研究进展. 西华师范大学学报: 自然科学版, 2004, 25(3): 298-303.
Tian M J. Review on the contributing factors to mineralization of soil nitrogen. Journal of China West Normal University: Natural Sciences, 2004, 25(3): 298-303. (in Chinese)
[6]    Stanford G. Effect of partial removal of soil organic N with sodium pyrophosphate in sulfuric acid solution on subsequent mineralization of nitrogen. Soil Science Society of America Journal, 1968, 32(5): 679-682.
[7]    金发会, 李世清, 卢红玲, 李生秀. 石灰性土壤供氮能力几种生物测定方法的评价研究. 中国农业科学, 2007, 40(7): 1422-1431.
Jin F H, Li S Q, Lu H L, Li S X. Estimation of the biological methods on assessing soil nitrogen-supplying capacity in calcareous soil. Scientia Agricultura Sinica, 2007, 40(7): 1422-1431. (in Chinese)
[8]    李菊梅, 王朝辉, 李生秀. 有机质、全氮和可矿化氮在反映土壤供氮能力方面的意义. 土壤学报, 2003, 40(2): 232-238.
Li J M, Wang Z H, Li S X. Significance of soil organic matter, total N and mineralizable nitrogen in reflecting soil N supplying capacity. Acta Pedologica Sinica, 2003, 40(2): 232-238. (in Chinese)
[9]    刘晓宏, 郝明德, 田梅霞. 土壤矿质氮和可矿化氮对当季作物的贡献. 土壤与环境, 2001, 10(3): 207-209.
Liu X H, Hao M D, Tian M X. Contribution of soil mineral nitrogen and soil mineralization nitrogen to seasonal crop. Soil and Environmental Sciences, 2001, 10(3): 207-209. (in Chinese)
[10]   Nuske A, Richter J. N-mineralization in loss-parabrownearthes: incubation experiments. Plant and Soil, 1981, 59: 237-247.
[11]   Standford G, Smith S J. Nitrogen mineralization potentials of soils. Soil Science Society of America Journal, 1972, 36(3): 465-472.
[12]   Meijboom F W, Hassink J, Van Noordwijk M. Density fractionation of soil macroorganic matter using silica suspensions. Soil Biology and Biochemistry, 1995, 27(8): 1109-1111.
[13]   Huygens D, Rütting T, Boeckx P, Van Cleemput O, Godoy R, Müller C. Soil nitrogen conservation mechanisms in a pristine south Chilean Nothofagus forest ecosystem. Soil Biology and Biochemistry, 2007, 39(10): 2448-2458.
[14]   Müller C, Stevens R J, Laughlin R J. A 15N tracing model to analyse N transformations in old grassland soil. Soil Biology and Biochemistry, 2004, 36(4): 619-632.
[15]   Deans J R, Molina A E, Clapp C E. Models for predicting potentially mineralizable nitrogen and decomposition rate constants. Soil Science Society of America Journal, 1986, 50(2): 323-326.
[16]   El- Gharous M, Westerman R L, Soitanpous P N. Nitrogen mineralizatin potential of arid and semiarid soils of Morocco. Soil Science Society of America Journal, 1990, 54(2): 438-443.
[17]   Curtin D, Wen G. Organic matter fractions contributing to soil nitrogen mineralization potential. Soil Science Society of America Journal, 1999, 63(2): 410-415.
[18]   徐明岗, 梁国庆, 张夫道. 中国土壤肥力演变. 北京: 中国农业科学技术出版社, 2006.
Xu M G, Liang G Q, Zhang F D. Soil Fertility Evolution in China. Beijing: China Agricultural Science and Technology Press, 2006. (in Chinese)
[19]   鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科学技术出版社, 2000.
Lu R K. Analysis Method of Soil and Agro-Chemistry. Beijing: China Agricultural Science and Technology Press, 2000. (in Chinese)
[20]   Steinweg J M, Fisk M C, McAlexander B, Groffman P M, Hardy J P. Experimental snowpack reduction alters organic matter and net N mineralization potential of soil macroaggregates in a northern hardwood forest. Biology and Fertility of Soils, 2008, 45(1): 1-10.
[21]   巨晓棠, 边秀举, 刘学军, 张福锁, 毛达如. 干旱土壤氮素矿化参数与氮素形态的关系. 植物营养与肥料学报, 2000, 6(3): 251-259.
Ju X T, Bian X J, Liu X J, Zhang F S, Mao D R. Relationship between soil nitrogen mineralization parameter with several nitrogen forms. Plant Nutrition and Fertilizer Science, 2000, 6(3): 251-259. (in Chinese)
[22]   Fox T R. Nitrogen mineralization following fertilization of douglas-fir forests with urea in western Washington. Soil Science Society of America Journal, 2004, 68(5): 1720-1728.
[23]   鲁彩艳, 牛明芬, 陈欣, 史奕, 石险峰. 不同施肥制度培育土壤氮矿化势与供氮潜力. 辽宁工程技术大学学报, 2007, 26(5): 773-775.
Lu C Y, Niu M F, Chen X, Shi Y, Shi X F. Nitrogen mineralization potentials of meadow brown soil in different fertilization practice. Journal of Liaoning Technical University, 2007, 26(5): 773-775. (in Chinese)
[24]   李世清, 李生秀. 有机物料在维持土壤微生物体氮库中的作用. 生态学报, 2001, 21(1): 136-142.
Li S Q, Li S X. Effects of organic materials on maintaining soil microbial biomass nitrogen. Acta Ecologica Sinica, 2001, 21(1): 136-142. (in Chinese)
[25]   杨巧丽, 姚拓, 王得武, 滚双宝. 木质纤维分解菌群筛选及其对秸秆分解与畜禽粪便除臭能力评价. 草业学报, 2015, 24(1): 196-203.
Yang Q L, Yao T, Wang D W, Gun S B. Screening of lignocelluloses degrading microbial communities for their ability to deodorize livestock and poultry wastes. Acta Prataculturae Sinica, 2015, 24(1): 196-203. (in Chinese)
[26]   李贵桐, 赵紫娟, 黄元仿, 李保国. 秸秆还田对土壤氮素转化的影响. 植物营养与肥料学报, 2002, 8(2): 162-167.
Li G T, Zhao Z J, Huang Y F, Li B G. Effect of straw returning on soil nitrogen transformation. Plant Nutrition and Fertilizer Science, 2002, 8(2): 162-167. (in Chinese)
[27]   张玉玲, 张玉龙, 虞娜, 姬景红. 长期不同施肥对水稻土有机氮素矿化特性影响的研究. 植物营养与肥料学报, 2008, 14(2): 272-276.
Zhang Y L, Zhang Y L, Yu N, Ji J H. Effect of different long-term fertilizing practices on nitrogen mineralization characteristic of paddy soil. Plant Nutrition and Fertilizer Science, 2008, 14(2): 272-276. (in Chinese)
[28]   Klemmedson J O, Rehfuess K E, Makeschin F, Rodenkirchen H. Nitrogen mineralization in lime- and gypsum-amended substrates from ameliorated acid forest soils. Soil Science, 1989, 147(1): 55-63.
[29]   Whalen J K, Bottomley P J, Myrold D D. Carbon and nitrogen mineralization from light- and heavey- fraction additions to soil. Soil Biology and Biochemistry, 2000, 32(10): 1345-1352.
[30]   Puget P, Chenu C, Balesdent J. Dynamics of soil organic matter associated with particle-size fractions of water-stable aggregates. European Journal of Soil Science, 2000, 51: 595-605.
[31]   Yamashita T, Flessa H, John B, Helfrich M, Ludwig B. Organic matter in density fractions of water-stable aggregates in silty soils: Effect of land use. Soil Biology and Biochemistry, 2006, 38(11): 3222-3234.
[32]   Tan Z, Lal R, Owens L, Izaurralde R C. Distribution of light and heavy soil organic carbon as related to land use and tillage practice. Soil and Tillage Research, 2007, 92: 53-59.
[33]   Six J, Paustain K, Eillott E T, Combrink C. Soil structure and organic matter: I. Distribution of aggregate-size classes and aggregate- associated carbon. Soil Science Society of America Journal, 2000, 64(2): 681-689.
[1] LIU MengYang, LIU Jie, CHEN Xiang, WANG QingYun, LUO LaiChao, QI YongBo, TIAN Da, LI JinCai, CHAI RuShan. Effects of Long-Term Straw Return on Distribution of Aggregates and Phosphorus Fractions in Shajiang Black Soil [J]. Scientia Agricultura Sinica, 2026, 59(3): 575-588.
[2] WANG RenZhuo, LI YueYing, HUANG ShaoMin, JIANG GuiYing, ZHANG Qi, LIU ChaoLin, YANG Jin, WANG MengRu, WANG BeiBei, LIU Fang, GUO DouDou, JIE XiaoLei, SONG Lian, LIU ShiLiang. Effects of Long-Term Combination of Organic and Inorganic Fertilizers on Bacterial Community Structure, Ecological Network, and Key Species in Fluvo-Aquic Soil [J]. Scientia Agricultura Sinica, 2026, 59(2): 354-367.
[3] WANG BingJie, QIN ShiHan, LI DeCheng, HU WenYou, JIANG Jun, CHI FengQin, ZHANG Chao, ZHANG JiuMing, XU YingDe, WANG JingKuan. Spatial Distribution Pattern and Transfer Function Construction of Soil Bulk Density in Nenjiang City, Heilongjiang Province [J]. Scientia Agricultura Sinica, 2025, 58(9): 1791-1803.
[4] WANG Zhao, ZHANG Bing, DONG SiQi, HU YuXi, QI ShuYu, FENG GuoZhong, GAO Qiang, ZHOU Xue. Effects of Long-Term Nitrogen Fertilizer Application on the Rhizosphere Microbial Community Structure and Function in Black Soil and Sandy Soil [J]. Scientia Agricultura Sinica, 2025, 58(3): 520-536.
[5] MA HeXiao, GE GuoLong, ZHANG XiangQian, LU ZhanYuan, WANG ManXiu, RONG MeiRen, SHI JingJing, ZHANG DeJian, SUN XuePing. Effects of Different Crop Rotation Systems on Soil Readily Oxidized Organic Carbon and Carbon Pool Activity Differences [J]. Scientia Agricultura Sinica, 2025, 58(24): 5201-5215.
[6] FANG KangRui, DING ShiJie, CHEN YuShan, YANG BingGeng, GUO TengFei, XU XinPeng, ZHAO ShiCheng, WANG XiuBin, HUANG ShaoMin, QIU ShaoJun, HE Ping, ZHOU Wei. In-Season Release Rate of Nitrogen and Phosphorus in Manure Fertilizers During the Wheat Season in Typical Fluvo-Aquic Soil Under the Combined Application of Chemical and Manure Fertilizers [J]. Scientia Agricultura Sinica, 2025, 58(24): 5234-5246.
[7] WU Yong, WEN Xue, WANG TianShu, HUANG YanYan, MENG YiLi, JIANG HongYu, BI LiDong, WU HuiJun, YAO ShuiHong. The Influence of Topographic Factors and Ridge Tillage Methods on Soil Nutrients and Fertility Index of Sloping Arable Land in the Black Soil Region [J]. Scientia Agricultura Sinica, 2025, 58(18): 3676-3689.
[8] GUO DouDou, ZHANG KeKe, HUANG ShaoMin, SONG Xiao, ZHANG ShuiQing, YUE Ke, DING ShiJie, GUO TengFei. Effects of Long-Term Fertilization on Phosphorus Adsorption and Desorption Characteristics of Fluvo-Aquic Soils [J]. Scientia Agricultura Sinica, 2025, 58(14): 2805-2820.
[9] WANG ShiJi, LI YueYing, CHEN Chen, JIANG GuiYing, LIU ChaoLin, ZHU ChangWei, YANG Jin, WANG MengRu, JIE XiaoLei, LIU Fang, LIU ShiLiang. The Characteristics of Ammonia Volatilization and Crop Yield Under Legume-Wheat Rotation System in Fluvo-Aquic Soil in Northern Henan Province [J]. Scientia Agricultura Sinica, 2025, 58(13): 2614-2629.
[10] ZHANG Yang, GAO Yan, ZHANG Yan, HUANG DanDan, CHEN XueWen, ZHANG ShiXiu, LIANG AiZhen. Effects of Residue Return Methods on Nitrogen Mineralization and N-Cycling Functional Genes in Black Soil of Northeast China [J]. Scientia Agricultura Sinica, 2025, 58(10): 1958-1968.
[11] DU JiaQi, ZHANG ZiWei, WANG RuoFei, LI Xing, GUO HongYan, YANG Shuo, FENG Cheng, HE TangQing, Giri Bhoopander, ZHANG XueLin. The Interactive Effects of Organic Fertilizer Substituting Chemical Fertilizers and Arbuscular Mycorrhizal Fungi on Soil Nitrous Oxide Emission in Shajiang Black Soil and Fluvo-Aquic Soil [J]. Scientia Agricultura Sinica, 2025, 58(1): 101-116.
[12] SHEN WenYan, ZHANG NaiYu, LI TianJiao, SONG TianHao, ZHANG XiuZhi, PENG Chang, LIU HongFang, ZHANG ShuXiang, DUAN BiHua. Characteristics of phoD-Harboring Microbial Communities Under Long-Term Fertilization and Its Effects on Organic Phosphorus Fractions in Black Soil [J]. Scientia Agricultura Sinica, 2024, 57(20): 4082-4093.
[13] LI TianJiao, ZHANG NaiYu, SHEN WenYan, SONG TianHao, LIU HongFang, LIU XiaoYan, ZHANG XiuZhi, PENG Chang, YANG JinFeng, ZHANG ShuXiang. Effects of Long-Term Fertilization on Soil Aggregate Stability and Its Driving Factors in Black Soil and Brown Soil [J]. Scientia Agricultura Sinica, 2024, 57(19): 3835-3847.
[14] LI LianYi, WANG ShiJi, JIANG GuiYing, LI Yang, YANG Jin, ZHU XuanLin, ZHU ChangWei, WANG RenZhuo, LIU Fang, JIE XiaoLei, LIU ShiLiang. Rotation Tillage Mode Improves Wheat Root and Yield in Fluvo- Aquic Soil in Norther Henan Province [J]. Scientia Agricultura Sinica, 2024, 57(18): 3626-3641.
[15] LIU YaJie, ZHANG TianJiao, ZHANG XiangQian, LU ZhanYuan, LIU ZhanYong, CHENG YuChen, WU Di, LI JinLong. Effects of Tillage Methods Under Straw Returning on the Labile Organic Carbon Fractions and Carbon Pool Management Index in Black Soil Farmland [J]. Scientia Agricultura Sinica, 2024, 57(17): 3408-3423.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd