Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1915-1922.doi: 10.3864/j.issn.0578-1752.2013.09.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Study on the Relationship Between Polymorphism of RERG Gene and Growth Traits of Three Goats Breeds in Guizhou

 CHEN  Zhi, LUO  Wei-Xing, LIU  Ruo-Yu, CAI  Hui-Fen, CHENG  Jie, SONG  Tao-Wei, ZHANG  Yi-Yu   

  1. 1.College of Animal Science, Guizhou University/Ministry of Education Key Laboratory for Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Guiyang 550025
    2.Agricultural Resources Technology Co., LTD of Changzhou Keli, Changzhou 213100, Jiangsu
  • Received:2012-10-10 Online:2013-05-01 Published:2013-01-18

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Abstract: 【Objective】This study aimed at researching polymorphism of the second, third and fourth exons of ras-related and estrogen-regulated growth inhibitor (RERG) gene in three indigenous Guizhou goats (Qianbei Ma goat, Guizhou White goat and Guizhou Black goat) and its association with growth traits was analyzed. 【Method】PCR-SSCP and PCR-direct sequencing were applied to detect the polymorphism sites and the general linear model was used to analyze its association with growth traits.【Result】Four consistent SNPs sites were found in the fourth exon of RERG gene in such three breeds. They are 56 bp C→G, 826 bp A→G, 1 434 bp T→C and 1 798 bp A→T. The following fixed effects model was employed in analyzing the growth traits in goat. The body height was significantly higher in individuals with genotype AB than AA and BB and genotype EF than EE and FF (P＜0.01). A significant difference was found in body weight between genotype CD and CC and DD (P＜0.05). The 1 798 bp (A/T) site did not appear significant difference. 【Conclusion】The 56 bp (C/G), 826 bp (A/G) and 1 434 bp (T/C) sites will provide a study basis between RERG genes and growth traits as a well as certain theoretical foundation for better feeding and genetic resources of indigenous goats.

Key words: RERG gene , indigenous Guizhou goats , PCR-SSCP , growth trait

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