Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (18): 3818-3825.doi: 10.3864/j.issn.0578-1752.2013.18.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Ammonium and Nitrate Nitrogen on Growth and Properties of 15N Distribution of Apple Trees

 LI  Jing, JIANG  Yuan-Mao, MEN  Yong-Ge, LI  Hong-Na, ZHOU  Le, WEI  Shao-Chong   

  1. College of Horticulture Science and Engineering，Shandong Agricultural University/State Key Laboratory of Crop Biology， Taian 271018，Shandong
  • Received:2013-06-26 Online:2013-09-15 Published:2013-07-08

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Abstract: 【Objective】 The study was carried out to investigate the growth and properties of 15N allocation and distribution in field grown apple trees with dwarfing interstock. 【Method】 The effectiveness of different forms of ammonium and nitrate nitrogen on growth and properties of 15N distribution of apple trees were studied by using 15N tracer on 1-year-old apple tree (Fuji/SH6/Malus hupehensis Pamp Rehd.).【Result】The results showed that 46 days after fertilization the utilization rate of 15NO3-15N was 3.43%, which significantly higher than 15NH4-15N, and also the growth vigor and root-shoot ratio. The 15NO3-15N partition ratio of base stock was 27.91%, which was also significantly higher than 15NH4-15N partition ratio of base stock of 25.13%. The nitrogen efficiency of NO3--N reduced till 118 days after fertilization, the growth vigor was significantly lower than NH4+-N，and there was no significant difference between the utilization rate of 15NO3-15N and 15NH4-15N. 15NO3-15N partition ratio of base stock (31.19%) was still significantly higher than 15NH4-15N partition ratio of base stock (25.10%). 【Conclusion】 Fertilizer efficiency of nitrate nitrogen was much faster than ammonium nitrogen, but there was no advance in long-term cultivation conditions.

Key words: apple , nitrate nitrogen , ammonium nitrogen , 15N , utilization , distribution

[1]李比希(德). 刘更另译. 化学在农业和生理学上的应用. 北京: 农业出版社, 1983.

Justus L (Freiherr von). Translated by Liu G L. Chemistry in Its Application to Agriculture and Physiology. Beijing: Agricultural Publishing House, 1983. (in Chinese)

[2]殷宏章. 罗宗洛文集. 北京: 科学出版社, 1988.

Yin H Z. The Collected Works of Luo Zongluo. Beijing: Science Press, 1988. (in Chinese)

[3]普里亚尼什尼科夫 (苏). 王天锋, 夏苏芳译. 在植物生活和苏联农业中的氮素. 北京: 科学出版社,1956.

Prianishnikov D N, Translated by Wang T F, Xia S F. Nitrogen in the Life of Plants and in the Agriculture of the U.S.S.R. Beijing: Science Press, 1956. (in Chinese) 

[4]张福锁, 崔振岭, 王激清, 李春俭, 陈新平.中国土壤和植物养分管理现状与改进策略, 植物学通报, 2007, 24 (6): 687-694.

Zhang F S, Cui Z L, Wang J Q, Li C J, Chen X P. Current status of soil and plant nutrient management in China and improvement strategies. Chinese Bulletin of Botany, 2007, 24 (6): 687-694. (in Chinese)

[5]刘权, 夏春森, 李式军.果树新品种 新技术 新进展. 北京：中国林业出版社, 1998: 188.

Liu Q, Xia C S, Li S J. New Cultivars and New Progress of Tree Fruits and Update Techniques for Orchard Management. Beijing: China Forestry Publishing House, 1998: 188. (in Chinese)

[6]顾曼如, 束怀瑞, 周宏伟. 苹果氮素营养研究Ⅴ. 不同形态15N的吸收、运转特性.山东农业大学学报, 1987,18（4）:17-24.

Gu M R, Shu H R, Zhou H W. A Study on the nitrogen nutrition of apple trees Ⅴ. The characteristics of absorption and transportation for various of 15N fertilizers. Journal of Shandong Agricultural University, 1987, 18 (4): 17-24. (in Chinese)

[7]王海宁, 葛顺峰, 姜远茂, 魏绍冲, 彭福田, 陈倩.苹果砧木生长季吸收利用硝态氮和铵态氮特性比较.园艺学报, 2012, 39(2): 343-348.   

Wang H N, Ge S F, Jiang Y M, Wei S C, Chen Q. Growth characteristics and absorption, distribution and utilization of 15NO3--N and 15NH4+-N application for five apple rootstocks. Acta Horticulturae Sinica, 2012, 39(2):343-348. (in Chinese)

[8]奚振邦. 硝态氮与铵态氮. 磷肥与复肥, 2011, 26(1): 62-65.

Xi Z B. Ammonium nitrogen and nitrate nitrogen. P Phosphate ＆ Compound Fertilizer, 2011,26(1):62-65. (in Chinese)

[9]曹翠玲.氮素及形态对作物的生理效应[D]. 陕西杨凌: 西北农林科技大学, 2002.

Cao C L. Effect of nitrogen and nitrogen form on physiological characteristics and yields of crop[D]. Yangling, Shaanxi: Northwest A & F University, 2002. (in Chinese)

[10]Arnon D L. Ammonium and nitrate nutrition of barley at different seasons in relation to hydrogen-ion concentration, manganese, copper, and oxygen supply. Soil Science, 1937, 44:91-121.     

[11]宋海星, 李生秀. 根系的吸收作用及土壤水分对硝态氮、铵态氮分布的影响.中国农业科学, 2005, 38(1):  96-101.

Song H X，Li S X. Effects of root uptake function and soil water on NO3--N and NH4+-N distribution. Scientia Agricultura Sinica, 2005, 38 (1): 96-101. (in Chinese) 

[12]钦绳武, 刘芷宇. 土壤-根系微区养分状况的研究Ⅵ.不同形态肥料氮素在根际的迁移规律．土壤学报, 1989, 26(2): 117-123. 

Qin S W, Liu Z Y. The nutrient status of soil-root interface Ⅵ. distribution of different fertilizer-N in rhizosphere soil. Acta Pedologica Sinica, 1989, 26(2): 117-123. (in Chinese)

[13]施卫明, 徐梦熊, 刘芷宇. 土壤-植物根系微区养分状况的研究Ⅳ.电子探针制样方法的比较及其应用. 土壤学报, 1987, 24(3): 286-290.

Shi W M, Xu M X, Liu Z Y. The nutrient status of soil-root interface Ⅳ. Study on sample preparation procedure for electron microprobe and its applications. Acta Pedologica Sinica, 1987, 24(3): 286-290. (in Chinese)

[14]白茹, 李丙智, 张林森, 高登涛, 王西玲, 韩明玉. 陕西渭北苹果园土壤矿质氮累积与分布状况研究. 西北林学院学报, 2006, 21(4): 50-53.

Bai R, Li B Z, Zhang L S, Gao D T, Wang X L, Han M Y. Mineral N distribution and accumulation in apple orchards of Weibei areas in Shaanxi  province. Journal of Northwest Forestry University, 2006, 21(4):50-53. (in Chinese)

[15]Vollbrecht P, Kasemir H I. Effects of exogenously supplied ammonium on root development of scots pine (Pinus sylvestris L. ) seedlings. Botanica Acta, 1992, 105 (4) :306-312.

[16]Bauer G A, Berntson G M. Ammonium and nitrate acquisition by plants in response to elevated CO2 concentration: the roles of root physiology and architecture. Tree Physiology, 2001, 21:137-144.

[17]徐季娥, 林裕益, 吕瑞江, 陈 良, 高占峰. 鸭梨秋施15N-尿素的吸收与分配. 园艺学报, 1993, 20 (2):145-149.

Xu J E, Lin Y Y, Lü R J, Chen L, Gao Z F. Studies on the absorption and the distribution of 15N-labelled urea to‘Yali’pear trees following autumn application. Acta Horticulturae Sinica, 1993, 20 (2):145-149. (in Chinese) 

[18]李皓, 张文, 赵旭勉, 任雪芹, 朱元娣. 苹果异戊烯基转移酶基因家族（MdIPTs）的克隆与MdIPT5a功能分析.中国农业科学, 2011, 44(19): 4029-4036. 

Li H, Zhang W, Zhao X M, Ren X Q, Zhu Y D.  Molecular cloning of isopentenyl transferases genes family in Malus domestica Borkh. and a preliminary functional analysis of MdIPT5a. Scientia Agricultura Sinica, 2011, 44(19): 4029- 4036. (in Chinese)

[19]Tian Q Y, Sun P, Zhang W H. Ethylene is involved in nitrate‐dependent root growth and branching in Arabidopsis thaliana. New Phytologist, 2009, 184: 918-931.

[20]Tian Q Y, Chen F J, Liu J X, Zhang F, Mi G. Inhibition of maize root growth by high nitrate supply is correlated with reduced IAA levels in roots. Journal of Plant Physiology, 2008, 165: 942-951.

[21]串丽敏, 赵同科, 安志装, 杜连凤, 李顺江, 马礼. 土壤硝态氮淋溶及氮素利用研究进展.中国农学通报, 2010, 26(11):200-205.

Chuan L M, Zhao T K, An Z Z, Du L F, Li S J, Ma L. Research advancement in nitrate leaching and nitrogen use in soils. Chinese Agricultural Science Bulletin, 2010, 26 (11): 200-205. (in Chinese)

[22]蔡晓布, 钱成. 氮肥形态和用量对藏东南地区烤烟产量和质量的影响. 应用生态学报, 2003, 14(1): 66-70.

Cai X B, Qian C. Effects of forms and application rate of nitrogen fertilizer on yield and qualities of tobacco in southeast Tibet. Chinese Journal of Applied Ecology, 2003, 14 (1):66-70. (in Chinese)

[23]Olsthoorn A F M, Keltjens W G, Van Baren B, Hopman M C G. Influence of ammonium on fine root development and rhizosphere pH of Douglas-fir seedling in sand. Plant and Soil, 1991, 133 : 75-81.

[24]高东升. “新红星”苹果植株对铵态氮及硝态氮反应特征的研究[D]. 山东泰安: 山东农业大学, 1992.

Gao D S. Studies on reaction characters of “starkrimson” apple trees to ammonium and nitrate nitrogen source[D]. Taian, Shandong: Shandong Agricultural University, 1992. (in Chinese)

[25]巨晓堂, 刘学军, 邹国元, 王朝辉, 张福锁.冬小麦/夏玉米轮作体系中氮素的损失途径分析, 中国农业科学, 2002, 35(12): 1493-1499.

Ju X T, Liu X J, Zou G Y, Wang C H, Zhang F S. Evaluation of nitrogen loss way in winter wheat and summer maize rotation system. Scientia Agricultura Sinica, 2002, 35(12): 1493-1499. (in Chinese)

[26]熊艳, 李永梅, 尹增松. 氮素形态在土壤中的转化及对烤烟产量的影响. 云南农业大学学报, 2003, 18(4): 332-336.

Xiong Y, Li Y M, Yin Z S. The conversion of different nitrogen forms in soil and their effects on the growth of flue-cured tobacco. Journal of Yunnan Agricultural University, 2003, 18(4): 332-336. (in Chinese)

[27]俞巧钢, 陈英旭, 张秋玲, 梁新强, 李华, 张志剑. DMPP对氮素垂直迁移转化及淋溶损失的影响.环境科学, 2007, 28(4): 813-818.

Yu Q G, Chen Y X, Zhang Q L, Liang X Q, Li H, Zhang Z J. Effect of DMPP on inorganic nitrogen transformation and leaching in vertical flow of simulated soil column. Environmental Science, 2007, 28(4): 813-818. (in Chinese)

[28]施木田, 陈少华. 园艺植物营养与施肥技术. 厦门: 厦门大学出版社, 2002: 37-38.

Shi M T, Chen S H. Horticultural Plant Nutrition and Fertilization Technique. Xiamen: Xiamen University Press, 2002: 37-38. (in Chinese)

[29]Thompson T, Doerge T A, Godin R E. Nitrogen and water interactions in subsurface drip-irrigated cauliflower: II Agronomic, economic, and environmental outcomes. Soil Science Society of America Journal, 2000, 64: 412-418.

[30]Wilcox G E, Hoff J E, Jones C M. Ammonium reduction of calcium and magnesium content of tomato and sweet corn leaf tissue and influence on incidence of blossom end rot of tomato fruit. Journal of the American Society for Horticultural Science, 1973, 98:86-89.

[31]Hohjo M, Kuwata C, Yoshikawa K, Ito T.  Effects of nitrogen form, nutrient concentration and Ca concentration on the growth, yield and fruit quality in NFT- tomato plants. Acta Horticulturae, 1995, 396:145-152.

[32]赵朋. 氮钙互作对日光温室黄瓜氮素代谢、品质和产量的影响机理的研究[D]. 山东泰安: 山东农业大学, 2008. 

Zhao P. Interactive effect of N and Ca on the nitrogen etabolism quality and yield of cucumber in solar-greenhouse [D]. Tai’an, Shandong: Shandong Agricultural University, 2008. (in Chinese)

[33]周鹏. 硝态氮与铵态氮对平邑甜茶实生苗激素水平和生长的影响[D]. 山东泰安: 山东农业大学, 2006.

Zhou P. Effect of nitrate and ammonium on the levels of hormones and growth of Malus hupenensis Rehd. seedlings[D]. Tai’an，Shandong: Shandong Agricultural University, 2006. (in Chinese)
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