Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (17): 3580-3586.doi: 10.3864/j.issn.0578-1752.2013.17.006

• PLANT PROTECTION • Previous Articles     Next Articles

The Expression Level of Alkaline Phosphatase (ALP1) in Cry1Ac-Resistant and Susceptible Cotton Bollworm Helicoverpa armigera Hübner

 ZHANG  Tao-12, ZHANG  Li-Li-2, WEI  Ji-Zhen-2, XIAO  Yu-Tao-2, LIANG  Ge-Mei-2, ZHOU  Rui-Yang-1   

  1. 1.College of Agriculture, Guangxi University, Nanning 530004
    2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193
  • Received:2013-05-06 Online:2013-09-01 Published:2013-07-01

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Abstract: 【Objective】 The objective of this study is to provide a theoretical basis for further clarifying Cry1Ac resistance mechanism and constituting reasonable resistance management strategy, the difference of ALP1 expression level between Cry1Ac-resistant and susceptible Helicoverpa armigera, the effect of Cry1Ac toxin on ALP1 expression level in Cry1Ac-resistant larvae and the relationship between ALP1 and Cry1Ac-resistant strains were analyzed. 【Method】 The expression levels of ALP1 in susceptible and Cry1Ac-resistant H. armigera larvae feeding on artificial diet containing Cry1Ac and no-Cry1Ac were compared using real-time quantitative PCR analysis. 【Result】 The ALP1 could express in whole development period of H. armigera, the highest expression level occurred in larvae, while the lowest was in pupae, and there was also relatively high expression level in moth. The highest expression level of ALP1 was in midgut of H. armigera larvae, followed by hindgut, foregut, malpighian tubules and epidermis. Compared with the susceptible H. armigera, the expression of ALP1 obviously increased in resistant larvae which had moderate resistance to Cry1Ac. The ALP1 expression of the resistant larvae significantly increased compared with the susceptible strain after they were fed on the artificial diet containing Cry1Ac, while the difference of ALP1 expression in 2nd and 3rd instar larvae was not significant between resistant strain and susceptible strain after they were fed on the normal artificial diet. The expression of ALP1 in the 4th instar larval midgut of all resistant strains were obviously higher than that in susceptible strain, and the expression level of ALP1 appeared downtrend along with the resistance ration increased. 【Conclusion】 The changes of ALP1 expression in midgut of resistant H. armigera may have a relationship with its resistance to Cry1Ac and metabolism of Cry1Ac.

Key words: Helicoverpa armigera , Bt , resistance , alkaline phosphatase , expression level

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