Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (17): 3571-3579.doi: 10.3864/j.issn.0578-1752.2013.17.005

• PLANT PROTECTION • Previous Articles     Next Articles

Anti-TMV Activity and Isolation of Active Ingredients in Fruit from Chaenomeles sinensis

 LIANG  Hong-Yan-1, RONG  Xu-1, CHEN  Pi-Ting-1, MA  Zhi-Qing-12, ZHANG  Xing-12   

  1. 1.Research & Development Center of Biorational Pesticides, Northwest A&F University/Shaanxi Research Center of Biopesticide Engineering & Technology, Yangling 712100, Shaanxi
    2.Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Yangling 712100, Shaanxi
  • Received:2013-02-06 Online:2013-09-01 Published:2013-05-06

Abstract: 【Objective】In order to find new botanical antiviral compounds, antiviral ingredients of Chaenomeles sinensis (Thouin) Koehne was isolated and evaluated against Tobacco mosaic virus (TMV). 【Method】Anti-TMV ingredients were isolated with bioassay-guided fractionation methods. Bioassay against TMV infection and replication of raw extracts and monomeric compound were determined using local lesion and potted plant methods on Nicotiana glutinosa and N. tabacum. 【Result】 Ethanol extract of C. sinensis displayed a significant inactivation effect on TMV in N. glutinosa with inhibition ratio of 97.76% and protection effect on TMV infection on N. tabacum var. K326 with efficiency of 49.98% at the concentration of 10 mg•mL-1. Ethyl acetate extract, n-butyl alcohol extract and water extract from ethanol extractum of C. sinensis all inactivated significantly TMV and the inhibition ratios were 89.02%, 82.84% and 84.74%. Dipentyl phthalate, β-amyrin, (8E)-hexadecenoic acid and (8E)-nonadecenoic acid were isolated and identified through comparison with data obtained via spectroscopic methods, and from values reported in the literature. Among them, β-Amyrin, (8E)-hexadecenoic acid and (8E)-nonadecenoic acid showed similar or better anti-MTV activities than 20% moroxydine hydrochloride•copper acetate WP, and the inhibition ratios were 27.51%, 33.33% and 41.08% at the concentration of 1.0 mg•mL-1. 【Conclusion】C. sinensis can be used as a biological antiviral agent in tobacco production and β-amyrin, (8E)-hexadecenoic acid and (8E)-nonadecenoic acid are its antiviral ingredients.

Key words: Chaenomeles sinensis , anti-TMV ingredients , β-amyrin , (8E)-hexadecenoic acid , (8E)-nonadecenoic acid

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