Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (11): 2353-2362.doi: 10.3864/j.issn.0578-1752.2013.11.020

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Identification of Silkworm Pupa-Specific Gene BmCP283 and Its Promoter

 CHENG  Dao-Jun, TANG  Lin, MENG  Meng, KANG  Li-Xia, WANG  Yong-Hu, PENG  Jian, MA  San-Yuan, XIA  Qing-You   

  1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715
  • Received:2012-11-19 Online:2013-06-01 Published:2012-12-31

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Abstract: 【Objective】The objective of this study is to identify genes that are specifically expressed during silkworm pupa-adult transition, clone their promoters and provide supports for controlling artificially pupa-adult transition and develope pupal bioreactor in silkworm (Bombyx mori).【Method】Silkworm pupa-specific genes were identified through analysis of microarray data for gene expression during silkworm metamorphosis and confirmed by RT-PCR experiments. The promoters of silkworm pupa-specific genes were cloned by PCR experiments and their driving activities and pupa-specificity were detected by transgenic technology.【Result】Cuticular protein gene BmCP283 was found to be specifically expressed at pupal stage in silkworm and was regarded as a pupa-specific gene. The potential promoter with a length of 2 004 bp on the upstream of the translational initial site of BmCP283 was cloned. Transgenic experiments confirmed that the promoter of BmCP283 could drive the red fluorescent gene dsRed to be specifically expressed at late pupal stages, which was similar to the expression profile of endogenous BmCP283. 【Conclusion】Both the expression pattern of BmCP283 and the activities of its promoter is pupa-specific in silkworm.

Key words: silkworm , pupa , promoter , specific , transgenic

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