Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (10): 2085-2093.doi: 10.3864/j.issn.0578-1752.2013.10.014

• HORTICULTURE • Previous Articles     Next Articles

Developments of Molecular Markers Tightly Linked to Fon-1 for Resistance to Fusarium oxysporum f. sp. niveum Race 1 in Watermelon

 ZHANG  Yi, ZHANG  Hai-Ying, GUO  Shao-Gui, REN  Yi, ZHANG  Jie, GENG  Li-Hua, LIANG  Zhi-Huai, XU  Yong   

  1. 1.Longping Branch of Graduate School, Central South University, Changsha 410125
    2.Beijing Vegetable Research Centre/ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China), Ministry of Agriculture, Beijing 100097
    3.Hu’nan Watermelon and Muskmelon Institute, Changsha 410125
  • Received:2012-12-02 Online:2013-05-15 Published:2013-03-27

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Abstract: 【Objective】 In order to provide technical support for molecular marker-assisted breeding for Fusarium wilt resistance in watermelon, molecular markers tightly linked to Fon-1 for Fusarium oxysporum f. sp. niveum race 1 resistance were developed.【Method】An F2 segregating population derived from a cross between the cultivated resistant male parent ‘Calhoun Gray’ and the susceptible female parent ‘Black Diamond’. The bulked segregant analysis (BSA) was employed to map the Fon-1 gene interval preliminaryly. A series of candidate single nucleotide polymorphism (SNP) sites that are linked to the target traits were obtained, and resequencing analysis within the target gene interval among 11 cultivated watermelon accessions was conducted. And then, the cleaved amplified polymorphic sequence (CAPS) or derived cleaved amplified polymorphic sequence (dCAPS) markers were designed to verify the linkage relationship between these CAPS/dCAPS marker and Fon-1 gene using F2 segregating population and germplasm resources. 【Result】 The preliminary results from screening of the resistant and susceptible DNA pools with 950 pairs of SSR/Indel/dCAPS primers mapped the Fon-1 gene to a 15 cM interval of watermelon chromosome 1. Three markers, 7716_fon, 7419_fon and 4451_fon, which were demonstrated to be tightly linked to Fon-1 gene, were developed using the SNP sites confirmed in the F2 population and 164 cultivars and the genetic distance between the markers and the Fon-1 gene was estimated to be 0.8 cM, 1.0 cM and 2.8 cM, respectively.【Conclusion】Three CAPS/dCAPS markers that can effectively distinguish the resistance/susceptibility to Fusarium oxysporum f. sp. niveum race 1 in cultivated watermelon were developed in this study and these markers will provide useful technical support for molecular breeding for resistance against F. oxysporum f. sp. niveum race 1 in cultivated watermelon.

Key words: watermelon , Fusarium wilt , race 1 , Fon-1 gene , CAPS/dCAPS marker

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