Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 774-785.doi: 10.3864/j.issn.0578-1752.2012.04.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Analysis of Sequence Characters of ADSL Gene and Correlation between Gene Expression and IMP Content in Duck

 XU  Shan-Jin, YU  De-Bing, WANG  Feng, HE  Zong-Liang, ZHANG  Jian-Feng, YU  Hai-Long, DU  Wen-Xing   

  1. 1.南京农业大学动物科技学院，南京 210095
    2.南京市畜牧家禽科学研究所，南京 210036
  • Received:2011-03-07 Online:2012-02-15 Published:2011-08-18

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Abstract: 【Objective】The CDS (coding sequences) of duck ADSL gene was cloned. The study was aimed to analyze gene sequences, protein structure, relative expression levels of ADSL gene in muscle and the correlation between gene expression and inosine monophosphate (IMP) content in ducks. This research revealed the ADSL gene structure of excellent native ducks of China, established a foundation for further research of its biological function and relationship with flavor substances. 【Method】 Reverse transcriptase PCR was employed to clone the cDNA of ADSL gene. The coding region sequences, protein structure and phylogenetic relationships were analyzed by bioinformatics software. real-time-PCR was employed to examine the relative expression levels of ADSL gene in muscle of ducks. IMP content in breast and leg muscle was detected by HPLC. 【Result】 The CDS of ADSL gene in ducks had 1380 bp in length, encoded 459 amino acid residues and showed 94.77% sequence identity with chicken. The physical and chemical properties of duck ADSL indicated that ADSL is a basic protein. And the protein has more strongly signal peptide and transmembrane segments in cells. The secondary structure of duck ADSL was mainly made by α helixes. Phylogenetic analysis showed that duck and chicken were in the same clustering, and then clustered with human, mice and other mammals, both of which had a distant relationship with fish and amphibian. The expression levels of ADSL gene among different varieties were extremely different (P＜0.01), that between female and male were extremely different (P＜0.01), and that among different locations were different (P＜0.05) except those in cherry valley ducks (P＞0.05). IMP content among different varieties were extremely different (P＜0.01), and among different sexes and locations were very different (P＜0.05). 【Conclusion】 The CDS of ADSL gene in ducks showed a high sequence identity with chicken. The expression levels of ADSL gene and the IMP content of relative locations were extremely different (P＜0.01) or different (P＜0.05) among different varieties, sexes and locations, respectively. ADSL gene relative expression and IMP content were significantly correlated.

Key words: duck, adenylosuccinate lyase, cloning, mRNA expression, IMP, high-performance liquid chromatography

[1]Zhang G Q, Ma Q G, Ji C. Effects of dietary inosinic acid on carcass characteristics, meat quality, and deposition of inosinic acid in broilers. Poultry Science, 2008, 87(7): 1364-1369.

[2]Wifall T C, Faes T M, Taylor-Burds C C, Mitzelfelt J D, Delay E R. An analysis of 5′-inosine and 5′-guanosine monophosphate taste in rats. Chemical Senses, 2007, 32(2): 161-172.

[3]Zikanova M, Skopova V, Hnizda A, Krijt J, Kmoch S. Biochemical and structural analysis of 14 mutant adsl enzyme complexes and correlation to phenotypic heterogeneity of adenylosuccinate lyase deficiency. Human Mutation, 2010, 31(4): 445-455.

[4]Ye M H, Chen J L, Zhao G P, Zheng M Q, Wen J. Correlation between polymorphisms in ADSL and GARS-AIRS-GART genes with inosine 5′-monophosphate(IMP) contents in Beijing-You Chickens. British Poultry Science, 2010, 51(5): 609-613.

[5]李慧芳, 陈宽维, 朱文奇, 薛素美, 宋卫涛, 王光瑛. 中国家鸭的分子遗传多态性及其起源. 农业生物技术学报, 2009, 17(5): 802-807.

Li H F, Chen K W, Zhu W Q, Xue S M, Song W T, Wang G Y. Molecular genetic polymorphism and origin of chinese domestic ducks. Journal of Agricultural Biotechnology, 2009, 17(5): 802-807. (in Chinese)

[6]戴  晔. 北京鸭和樱桃谷鸭肉用性能及MSTN基因多态性的研究[D]. 陕西: 西北农林科技大学, 2006.

Dai Y. Study on meat traits and the polymorphism of MSTN gene with peking ducks and Cherry Valley Ducks[D]. Shaanxi: Northwest A & F University of, 2006. (in Chinese)

[7]张学余, 束婧婷, 季从亮, 陈国宏, 韩　威. 5个鸡种腺苷琥珀酸裂解酶(ADSL)基因外显子9 SNP及其与鸡肉肌苷酸含量的相关分析. 畜牧兽医学报, 2007, 38(3): 237-240.

Zhang X Y, Su J T, Ji C L, Chen G H, Han W. Correlation analysis between single nucleotide polymorphism of the ADSL exon 9 and inosine monophosphate acid content in five chicken breeds. Acta Veterinaria et Zootechnica Sinica, 2007, 38(3): 237-240. (in Chinese)

[8]Marie S, Race V, Nassogne M C, Vincent M F, van den Berghe G. Mutation of a nuclear respiratory factor 2 binding site in the 5′ untranslated region of the ADSL gene in three patients with adenylosuccinate lyase deficiency. American Journal of Human Genetics, 2002, 71(1): 14-21.

[9]Tabucchi A, Carlucci F, Rosi F, Guerranti R, Marinello E. Determination activity and biological role of adenylosuccinate lyase in blood cells. Biomed Pharmacother, 2001, 55: 277-283.

[10]Wong L J C, O’Brien W E. Characterization of the cDNA and the gene encoding murine adenylosuccinate lyase. Genomics, 1995, 20: 341-343.

[11]Kmoch S, Hartmannová H, Stib?rková B, Krijt J, Zikánová M, Šebesta I. Human adenylosuccinate lyase(ADSL), cloning and characterization of full-length cDNA and its isoform, gene structure and molecular basis for ADSL deficiency in six patients. Human Molecular Genetics, 2000, 9(10): 1501-1513.

[12]Aimi J, Badylak J, Williams J, Chen Z D, Zalkin H. Dixon J E. Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli. Journal of Biological Chemistry, 1990, 16(5): 9011-9014.

[13]Spiegel E K, Colman R F, Patterson D. Adenylosuccinate lyase deficiency. Molecular Genetics and Metabolism, 2006, 89(1/2): 19-31.

[14]Gitiaux C, Ceballos-Picot I, Marie S, Valayannopoulos V, Rio M, Verrieres S, Benoist J F, Vincent M F, Desguerre I, Bahi-Buisson N. Misleading behavioural phenotype with adenylosuccinate lyase deficiency. European Journal of Human Genetics, 2009, 17: 133-136.

[15]Race V, Marie S, Vincent M F, van den Berghe G. Clinical, biochemical and molecular genetic correlations in adenylosuccinate lyase deficiency. Human Molecular Genetics, 2000, 9(14): 2159-2165.

[16]Marie S, Cuppens H, Heuterspreute M, Jaspers M, Tola E Z, Gu X X, Legius E, Vincent M F, Jaeken J, Cassiman J J, van den Berghe G. Mutation analysis in adenylosuccinate lyase deficiency, eight novel mutations in the re-evaluated full ADSL coding sequence. Human Mutation, 1999, 13: 197-202.

[17]Chen J L, Zhao G P, Zheng M Q, Wen J, Yang N. Estimation of genetic parameters for contents of intramuscular fat and inosine-5′-monophosphate and carcass traits in Chinese Beijing-You chickens. Poultry Science, 2008, 87: 1098-1104.

[18]刘望夷, 竺来发, 翁志发, 沈洪民. 肉用鸡肌肉中肌苷酸含量的比较. 中国农业科学, 1980(4): 79-83.

Liu W Y, Zhu L F, Weng Z F, Shen H M. A comparative study of inosinic acid contents in chicken muscle. Scientia Agricultura Sinica, 1980(4): 79-83. (in Chinese)

[19]Iwamoto E, Oka A, Iwaki F. Effects of the fattening period on the fatty acid composition of fat deposits and free amino acid and inosinic acid contents of the longissimus muscle in carcasses of Japanese Black steers. Journal of Animal Science, 2009, 80: 411-417.

[20]陈国宏, 侯水生, 吴信生, 王克华, 陈宽维, 李建凡. 中国部分地方鸡肌肉肌苷酸含量研究. 畜牧兽医学报, 2000, 31(3): 211-215.

Chen G H, Hou S S, Wu X S, Wang K H, Chen K W, Li J F. Comparison between inosinic acid content of muscle in chinese native chickens. Chinese Journal of Animal and Veterinary Sciences, 2000, 31(3): 211-215. (in Chinese)

[21]Shu J T,  Bao W B,  Zhang H X, Zhang X Y, Ji C L, Chen G H. Correlation between adenylosuccinate lyase(ADSL) gene polymorphism and inosine monophosphate acid(IMP) content in domestic fowl and genetic relationship between red jungle fowl and domestic fowl. Hereditas, 2007, 29(3): 343-348.

[22]Shu J T,  Bao W B,  Zhang H X, Ji C J, Han W, Chen K W. Combined effect of mutations in ADSL and GARS-AIRS-GART genes on IMP content in chickens. British Poultry Science, 2009, 50(6): 680-686.

[23]陈继兰. 肌肉肌苷酸和肌内脂肪含量遗传规律及相关候选基因的研究[D]. 北京: 中国农业大学, 2004.

Chen J L. Studies on inheritance and candidate genes of inosine-5′- monophosphate and intramuseular fat contents in chicken meat[D]. Bei jing: China Agricultural University, 2004. (in Chinese)

[24]Zhang Y, Morar M, Ealick S E. Structural biology of the purine biosynthetic pathway. Cellular and  Molecular Life Sciences, 2008, 65(23): 3699-3724.

[25]Lu J, Sivamani E, Azhakanandam K, Samadder P, Li X, Qu R. Gene expression enhancement mediated by the 5′ UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants. Molecular Genetics and Genomics, 2008, 279(6): 563-572.

[26]Cho K H, Kim M J, Jeon G J, Chung H Y. Association of genetic variants for FABP3 gene with back fat thickness and intramuscular fat content in pig. Molecular Biology Reports, 2010, 17: 1-6.

[27]Shu J T, Bao W B, Zhang X Y, Zhang H X, Chen G H. Association and haplotype analysis of purH gene with inosine monophosphate content in chickens. Animal Biotechnology, 2008, 19: 310-314.

[28]Zheng G X Y, Ravi A, Calabrese J M, Medeiros L A, Kirak O, Dennis L M, Jaenisch R, Burge C B, Sharp P A. A latent pro-survival function for the mir-290-295 cluster in mouse embryonic stem cells. PLoS Genetics, 2011. Doi:10.1371/journal.pgen.1002054.

[29]Coste B, Mathur J, Schmidt M, Earley T J, Ranade S, Petrus M J, Dubin A E, Patapoutian A. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Science, 2010, 330(6000): 55-60.
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