Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 768-773.doi: 10.3864/j.issn.0578-1752.2012.04.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Genetic Variation of ESR Gene in Different Generations of Sujiang Pigs and the Impact on Reproductive Traits of Pigs

 WANG  Xiao-Yan, HE  Qing-Ling, JING  Rong-Bin, SONG  Cheng-Yi   

  1. 1.扬州大学动物科学与技术学院，江苏扬州 225009
  • Received:2011-10-17 Online:2012-02-15 Published:2011-12-31

PDF

591

Abstract: 【Objective】This study was aimed to analyze PvuⅡ polymorphism of ESR gene in different generations of Sujiang pigs and the correlation between the polymorphism and the reproductive traits of Sujiang pigs. 【Method】 PCR-RFLP was used to diagnose polymorphism of ESR gene in 906 Sujiang pigs of 2-6 generations. Least square method and one-way anova were performed to evaluate the association of this polymorphism site with reproductive traits of pigs. 【Result】 The frequency of allele B of ESR gene in Sujiang pigs of 2-6 generations showed an increasing trend. Pigs with BB genotypes appeared in the third generations and then presented an upward trend in the frequency. There was no significant relationship between the polymorphism of ESR gene and litter size of sows mated to different genotypes of boars. But they had significant impacts on litter size that different genotypes of boars mated with different genotypes of sows (P＜0.05). 【Conclusion】 The selection of boars for reproductive traits of Sujiang pig must be taken into consideration.

Key words: ESR gene, Sujiang pig, genetic variation, reproductive traits

[1]经荣斌. 江苏省地方猪种的保存和选育的演变. 猪业科学,  2009(2): 96-99.

Jing R B. The evolvement of preservation and breeding of native pig breeds in Jiangsu province. Swine Industry Science, 2009(2): 96-99. (in Chinese)

[2]Xing Y, Ren J, Ren D, Guo Y, Wu Y, Yang G, Mao H, Brenig B, Huang L. A whole genome scanning for quantitative trait loci on traits related to sperm quality and ejaculation in pigs. Animal Reproduction Science, 2009, 114(1-3): 210-218.

[3]Rothschild M F, Jacobson C, Vaske D A, Tuggle C K, Wang L, Short T H, Eckardt G R, Sasaki S, Vincent A M, Mclaren D G, Southwood O, van der Steen H, Mileham A, Plastow G. The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proceedings of the National Acadamy of Sciences, 1996, 93: 201-205.

[4]Short T H, Rothschild M F, Southwood O I, Mclaren D G, de Vries A, van der Steen H, Eckardt G R, Tuggle C K, Helm J, Vaske D A, Mileham A J, Plastow G S. Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines. Journal of Animal Science, 1997, 75(12): 3138-3142.

[5]Noguera J L, Varona L, Gómez-Raya L, Sánchez A, Babot D, Estany J, Messer L A, Rothschild M, Pérez-Enciso M. Estrogen receptor polymorphism in Landrace pigs and its association with litter size performance. Livestock, 2003, 82(1): 53-59.

[6]兰旅涛, 周利华, 李  琳, 陈东军. 3个外来种猪群ESR基因位点多态性及其与繁殖性能相关性分析. 江西农业大学学报, 2006, 28(1): 115-118.

Lan L T, Zhou L H, Li L, Chen D J. Polymorphism of ESR gene and its relationship to reproduction performance in three western commercial pig breeds herds. Acta Agriculturae Universitis Jiangxiensis, 2006, 28(1): 115-118. (in Chinese)

[7]施启顺, 柳小春, 刘志伟, 李小平, 李永辉, 唐  凡. 5个与猪产仔数相关基因的效应分析. 遗传, 2006, 28(6): 652-658.

Shi Q S, Liu X C, Liu Z W, Li X P, Li Y H, Tang F. Effect of five genetic loci related to pig litter size. Hereditas, 2006, 28(6): 652-658.

[8]陈克飞, 黄路生, 李  宁, 张  勤, 罗  明, 吴常信. 猪雌激素受体(ESR)基因对产仔数性状的影响. 遗传学报, 2000, 27(10): 853-857.

Chen K F, Huang L S, Li N, Zhang Q, Luo M, Wu C X. The genetic effect of estrogen receptor(ESR) on litter size traits in pig. Acta Genetica Sinica, 2000, 27(10): 853-857. (in Chinese)

[9]徐宁迎, 章胜乔, 彭淑红. 金华猪3个繁殖性状主基因的分布及其效应的研究. 遗传学报, 2003, 30(12): 1090-1096.

Xu N Y, Zhang S Q, Peng S H. Investigation on the distribution and their effects on reproduction traits of three major genes in Jinhua pigs. Acta Genetica Sinica, 2003, 30(12): 1090-1096. (in Chinese)

[10]Linville R C, Pomp D, Johnson R K, Rothschild M F. Candidate gene analysis for loci affecting litter size and ovulation rate in swine, Journal of Animal Science, 2001,79: 60-67.

[11]Progemuller C, Hamann H, Distl O. Candidate gene markers for litter size in German pig lines. Journal of Animal Science, 2001, 79: 2565-2570.

[12]Goliasova E, Wolf J. Impact of the ESR gene on litter size and production traits in Czech Large White pigs. Animal Genetics, 2004, 35(4): 293-297.

[13]Bárbara A, Fernando H B, Robson C A, Mauricio B, Mauricio M F, Luiz R G. Association of the estrogen receptor gene PvuII restriction polymorphism with expected progeny differences for reproductive and performance traits in swine herds in Brazil. Genetics and Molecular Biology, 2006, 29(2): 273-277.

[14]孙丽亚, 王宵燕, 宋成义, 赵  芹, 谢  飞, 吴  晗, 李碧春. 苏姜猪ESR基因和FSHβ基因的多态性与繁殖性能的相关性研究. 安徽农业科学, 2008, 36(13): 5457-5458.

Sun L Y, Wang X Y, Song C Y, Zhao Q, Xie F, Wu H, Li B C. Study on the correlation between the polymorphism of ESR gene and FSHβ gene in Sujiang pig and its reproductive performance. Journal of Anhui Agricultural Sciences, 2008, 36(13): 5457-5458. (in Chinese)

[15]王宵燕, 曹国林, 孙丽亚, 宋成义, 霍永久, 经荣斌. 苏姜猪ESR基因和FSHβ基因的多态性及其对部分生长性状的影响. 中国畜牧杂志, 2009, 13: 1-3.

Wang X Y, Cao G L, Sun L Y, Song C Y, Huo Y J, Jing R B.  Polymorphism of ESR and FSHβ genes and their effects on partial growth traits of Sujiang pigs. Chinese Journal of Animal Science, 2009, 13: 1-3. (in Chinese)

[16]Sambrook J, David W, Russell. 分子克隆实验指南. 北京: 科学出版社, 2002: 463-469.

Sambrook J, David W, Russell. Guide to Molecular Cloning Experiments. Beijing: Science Press, 2002: 463-469. (in Chinese)

[17]Carreau S, Hess R A. Oestrogens and spermatogenesis. Philosophical Transactions of the Royal Society London. B, 2010, 365(1546): 1517-1535.

[18]Rochira V, Granata A R, Madeo B, Zirilli L, Rossi G, Carani C. Estrogens in males: what have we learned in the last 10 years? Asian Journal of Andrology, 2005, 7: 3-20.

[19]Eddy E M, Washburn T F, Bunch D O, Goulding E H, Gladen B C, Lubahn D B, Korach K S. Targeted disruption of the estrogen receptor gene in male mice causes alteration of spermatogenesis and infertility. Endocrinology, 1996, 137(11): 4796-4805.

[20]Chen M, Hsu I, Wolfe A, Radovick S, Huang K, Yu S, Chang C, Messing E M, Yeh S. Defects of prostate development and reproductive system in the estrogen receptor-alpha null male mice. Endocrinology, 2009, 150: 251-259.

[21]Hess R A. Estrogen in the adult male reproductive tract: a review. Reproductive Biology Endocrinology, 2003, 1: 52.

[22]Gunawan A, Kaewmala K, Uddin M J, Cinar M U, Tesfaye D, Phatsara C, Tholen E, Looft C, Schellander K. Association study and expression analysis of porcine ESR1 as a candidate gene for boar fertility and sperm quality. Animal Reproduction Science, 2011, 128(1/4): 11-21.

[23]Guarducci E, Nuti F, Becherini L, Rotondi M, Balercia G, Forti G, Krausz C. Estrogen receptor alpha promoter polymorphism: stronger estrogen action is coupled with lower sperm count. Human Reproduction, 2006, 21(4): 994-1001.

[24]Safranski T J. Genetic selection of boars. Theriogenology, 2008, 70: 1310-1316.

[25]Robinson J A, Buhr M M. Impact of genetic selection on management of boar replacement, Theriogenology, 2005, 63: 668-678.

[26]Bolet G, Bidanel J P, Ollivier L. Selection for litter size in pigs. II. Efficiency of closed and open selection lines. Genetics Selection Evolution, 2001, 33: 515-528.

[27]Foote R H. Within-herd use of boar semen at 5 degrees C, with a short note on electronic monitoring of oestrus. Reproduction in Domestic Animals, 2002, 37: 61-63.

[28]William L F. Genetic and Phenotypic variation in reproductive traits of AI boars. Theriogenology, 2008, 70: 1297-1303.
[1] HUANG XunHe,WENG ZhuoXian,LI WeiNa,WANG Qing,HE DanLin,LUO Wei,ZHANG XiQuan,DU BingWang. Genetic Diversity of Indigenous Yellow-Feathered Chickens in Southern China Inferred from Mitochondrial DNA D-Loop Region [J]. Scientia Agricultura Sinica, 2022, 55(22): 4526-4538.
[2] ZHANG LinLin,ZHI Hui,TANG Sha,ZHANG RenLiang,ZHANG Wei,JIA GuanQing,DIAO XianMin. Characterizations of Transcriptional and Haplotypic Variations of SiTOC1 in Foxtail Millet [J]. Scientia Agricultura Sinica, 2021, 54(11): 2273-2286.
[3] QIN YanHong,WANG YongJiang,WANG Shuang,QIAO Qi,TIAN YuTing,ZHANG DeSheng,ZHANG ZhenChen. Complete Nucleotide Sequence Analysis and Genetic Characterization of the Sweet potato feathery mottle virus O and RC Strains Isolated from China [J]. Scientia Agricultura Sinica, 2020, 53(11): 2207-2218.
[4] WU BingChao, TONG Lei, DU ZhaoChang, HU JiaLing, ZHANG Huan, CHEN Yi, LIU Wei, ZHANG XinQuan, HUANG LinKai. Mutagenic Effects of 60Co-γ-rays on Two Species of Pennisetum Genus Forages [J]. Scientia Agricultura Sinica, 2019, 52(3): 414-427.
[5] LI ChunJia, QIN Wei, XU ChaoHua, LIU HongBo, MAO Jun, LU Xin. Genetic Variations and Cluster Analysis of Photosynthetic Gas Exchange Parameters in Exotic Sugarcane Cultivars [J]. Scientia Agricultura Sinica, 2018, 51(12): 2288-2299.
[6] WANG YaFei, RUAN Tao, ZHOU Yan, WANG XueFeng, WU GenTu, SUN XianChao, ZHOU ChangYong, QING Ling. Genetic Variation of p20 of the Severe and Mild Strains of CTV in the Sweet Orange and Pummelo [J]. Scientia Agricultura Sinica, 2017, 50(7): 1343-1350.
[7] WANG Lin, LI XinFeng, XU YuMei, CHANG YinDong, WANG JianMing. Analysis of Population Distribution and Genetic Variation of Plant Pathogenic Fusarium in Shanxi Province [J]. Scientia Agricultura Sinica, 2017, 50(10): 1802-1816.
[8] ZHAO PeiFang, ZHAO Jun, LIU JiaYong, ZAN FengGang, XIA HongMing, P.A. Jackson, J. Basnayake, N.G. Inman-Bamber, YANG Kun, ZHAO LiPing, QIN Wei, CHEN XueKuan, ZHAO XingDong, FAN YuanHong. Genetic Variation of Four Physiological Indexes as Impacted by Water Stress in Sugarcane [J]. Scientia Agricultura Sinica, 2017, 50(1): 28-37.
[9] GENG Li-ying, ZHANG Chuan-sheng, ZHAO Shu-yu, CHEN Juan, GONG Yuan-fang, LIU Zheng-zhu, ZHU Wen-jin, LI Xiang-long . Polymorphism of Pre-microRNA-1658 Gene in Chicken [J]. Scientia Agricultura Sinica, 2015, 48(19): 3919-3930.
[10] LIU Zhi-zhai, WU Xun, LI Yong-xiang, DING Xiao-yu, WANG Feng-ge, SHI Yun-su, SONG Yan-chun, ZHAO Jiu-ran, LI Yu, WANG Tian-yu. Assessment of Genetic Variation in Different Races of Maize Landraces in China [J]. Scientia Agricultura Sinica, 2015, 48(16): 3101-3111.
[11] SONG Tao-wei, CAI Hui-fen, LUO Wei-xing, LIU Ruo-yu, ZHANG Yi-yu, SUN Yan-yan, LIU Bin. Association of GHSR and GHRL Gene Genetic Variation with Growth Traits in Two Guizhou Goat Breeds [J]. Scientia Agricultura Sinica, 2015, 48(1): 140-153.
[12] CHEN Xue-Sen, ZHANG Jing, LIU Da-Liang, JI Xiao-Hao, ZHANG Zong-Ying, ZHANG Rui, MAO Zhi-Quan, ZHANG Yan-Min, WANG Li-Xia, LI Min. Genetic Variation of F1 Population Between Malus sieversii f. neidzwetzkyana and Apple Varieties and Evaluation on Fruit Characters of Functional Apple Excellent Strains [J]. Scientia Agricultura Sinica, 2014, 47(11): 2193-2204.
[13] WANG Ji-Ying, WANG Hai-Xia, CHI Rui-Bin, GUO Jian-Feng, WU Ying. Progresses in Research of Genome-Wide Association Studies in Livestock and Poultry [J]. Scientia Agricultura Sinica, 2013, 46(4): 819-829.
[14] LI Su-Ya, ZHANG Jian-Hong, CHEN Wen, HUANG Yan-Qun, SUN Gui-Rong, HAN Rui-Li, KANG Xiang-Tao. Genetic Variation Analysis of 3′-UTR Region of Chicken Lpin1 Gene and the Potential Effect on miRNA Binding Sites [J]. Scientia Agricultura Sinica, 2012, 45(8): 1613-1620.
[15] WU Xiu-xiang; SHI Xue-kui; WU Hai-tao; MAO Yong-jiang; YANG Zhang-ping;LI Jun-ya; GAO Hui-jiang. Genetic Variation in CAPN1 Gene and Its Association with Carcass Traits in Chinese Cattle [J]. Scientia Agricultura Sinica, 2011, 44(7): 1466-1473.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd