Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (24): 4978-4987.doi: 10.3864/j.issn.0578-1752.2012.24.003

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effects of Chilling Stress on Protein and Related Gene Expression in Chloroplasts of Sugarcane

 HUANG  Qiao-Ling, HUANG  Xing, SUN  Fu, SUN  Bo, YANG  Li-Tao, LI  Yang-Rui   

  1. 1.College of Agriculture, Guangxi University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Nanning 530005
    2.Sugarcane Research Center, Chinese Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement Guangxi, Ministry of Agriculture/Guangxi Key Laboratory of Crop Genetic Improvement and Biotechnology/Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007
  • Received:2012-07-16 Online:2012-12-15 Published:2012-09-19

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Abstract: 【Objective】 The present study was conducted to investigate the effects of chilling stress on chloroplasts at protein and mRNA levels to provide references for further investigation on molecular mechanism of chilling stress in sugarcane.【Method】Sugarcane varieties GT28 (strong cold resistant sugarcane variety) and ROC22 (weak cold resistant sugarcane variety) were employed as the plant materials, and treated for 9 d, 15 d and 24 d of chilling stress, respectively, and then the chloroplast protein was extracted and used for electrophoresis analysis, and expressions of the genes were analyzed using real-time PCR.【Result】The results of electrophoresis analysis showed that, for both sugarcane varieties, the relative content of chloroplast protein decreased with the chilling time extension. The protein bands of 39.92, 32.95 and 22.87 kD were down-regulated obviously. Mass spectrometry revealed that they were ATP synthase subunit gamma, oxygen-evolving enhancer protein 1 and chloroplast 23 kD polypeptide of photosystem II, and they were encoded by atpC, psbO and psbP genes, respectively. Their fragment length was 937, 996, and 721 bp, respectively. The obtained fragment of psbO gene was its complete open reading frame, which has been registered in the GenBank with the accession number JQ898540. Real-time PCR analysis showed that chilling stress suppressed the expression of atpC gene, but induced the expressions of psbO and psbP genes, which reached the maximum at the time point of 15 d. 【Conclusion】 Generally, chilling stress promoted protein degradation, and the protein declined more in ROC22 than that in GT28. Chilling stress suppressed the expression of atpC gene, but induced the expressions of psbO and psbP genes in chloroplasts of sugarcane.

Key words: chilling stress , sugarcane , chloroplast , proteome , differential expression

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