Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (21): 4343-4350.doi: 10.3864/j.issn.0578-1752.2012.21.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Arabidopsis TUA2 Gene Mediates Seed Germination Under ABA Stress

 LIU  Hai-Hao, WU  Li-Zhu, YUE  Zhi-Liang, PAN  Yan-Yun   

  1. 1.河北农业大学生命科学学院，河北保定 071000
  • Received:2012-06-11 Online:2012-11-01 Published:2012-08-28

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Abstract: 【Objective】This study would characterize the TUA2 gene involving in seed germination under ABA stress in Arabidopsis by the method of reverse genetics. 【Method】 The Arabidopsis T-DNA inserted into TUA2 gene mutants were obtained from ABRC. The T-DNA insertion sites of mutants were analyzed by Tail PCR. Over-expression constructs of TUA2 were introduced into Arabidopsis by an Agrobacterium-mediated method. RT-PCR was used to detect TUA2 and ABA-responsive genes accumulation in mutants and transgenic plants. The seed germination of transgenic and mutant lines was tested on MS medium with different concentrations of ABA. 【Result】 The T-DNA was inserted into the gene promoter region in mutants tua2-1 and tua2-2, and led to the increasing accumulation of the TUA2. In the medium of MS/0.8 μmol•L-1 ABA, the seed germination rate of wild type was 76% after five days, whereas all the TUA2 over-expression lines, mutants and transgenic plants, were 6%-18%. The expression of ABII, HAB and P5CS was induced by ABA in wild type, but not in mutants and transgenics. 【Conclusion】 The T-DNA insertion of the mutants tua2-1 and tua2-2 resulted in the increasing expression of TUA2 gene. The TUA2 gene might be involved in plant responses to ABA by mediating the transcription of ABA-responsive genes.

Key words: Arabidopsis thaliana, seed germination, TUA2, ABA

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