Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 4092-4101.doi: 10.3864/j.issn.0578-1752.2012.19.022

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Correlations Between SNP of MTNR Gene and Cashmere-Producing Traits of Inner Mongolia White Cashmere Goat

 LIU  Bin, ZHAO  Cun-Fa, LI  Yu-Rong, GAO  Feng-Qin, GAO  Wa, CHENG  Li-Xin, MENG  Ke-Ba-Ya-尔, MA  Yue-Jun, LIU  Shao-Qing, LI  Jin-Quan   

  1. 1.内蒙古农业大学动物科学学院，呼和浩特 010018
    2.内蒙古自治区农牧业科学院，呼和浩特010031
    3.中国农业科学院草原研究所， 呼和浩特 010010
    4.鄂托克前旗农牧业局，内蒙古鄂尔多斯 016200；5内蒙古白绒山羊种羊场，内蒙古鄂尔多斯 016100
  • Received:2012-02-27 Online:2012-10-01 Published:2012-04-11

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Abstract: 【Objective】Correlations between SNP of cashmere goat melatonin receptor (MTNR) gene and cashmere-producing traits were studied to find molecular marker of MAS for molecular breeding.【Method】 Arbas white cashmere goats and partial sequences of MTNR1a and 1b genes were used as experimental samples and candidate genes, respectively. PCR-SSCP and DNA sequencing technologies were conducted to detect their single nucleotide polymorphism. Results were analyzed by least square mean (LSM) among production traits of genotypes.【Result】 There was no SSCP in the exons 1 and 2 of MTNR1a gene and the exon 1 of MTNR1b gene, while there was the SSCP in the exon 2 of MTNR1b gene and three of SNP were detected. P4 primer loci existed in C-G mutation of NM_001206907:g.648 bp which didn’t result in change of amino acid, and G-A mutation of NM_001206907:g.665 bp which led to CGC-CAC of the 223rd codon from Arg into His. The results showed that polymorphism loci had an significant effect on cashmere yield (P＜0.05), and cashmere yield of AA genotype increased by 98.78 g and 148.23 g, as compared to AB and BB, respectively. Meanwhile, there was a significant correlation (P＜0.05) in interaction effect between the polymorphism loci genotype and age cashmere thickness. P5 primer loci existed in G-A mutation of NM_001206 907:g.1015 bp which led to GGC-AGC of the 339th codon from Gly into Ser. The analyses indicated that interaction effect of the polymorphism loci genotype and age was the marginal significant correlation (P＜0.1) with cashmere fineness. P4 and P5 primes locus were not significantly (P＞0.05) with kidding number and birth weight.【Conclusion】P4-AA genotype can be used as molecular marker of MAS for cashmere-producing traits of Inner Mongolian Arbas white cashmere goats.

Key words: cashmere goat, MTNR gene, SNP, cashmere-producing traits

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