Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (18): 3812-3819.doi: 10.3864/j.issn.0578-1752.2012.18.014

• HORTICULTURE • Previous Articles     Next Articles

Genetic Presentation of BC1 Between ‘Zhongshanjingui’ and ‘Zhongshanjingui’ × Ajania przewalskii

 ZHU  Wen-Ying, LIU  Xin-Chun, FANG  Wei-Min, GUAN  Zhi-Yong, CHEN  Su-Mei, JIANG  Jia-Fu, CHEN  Fa-Di   

  1. 1.南京农业大学园艺学院，南京 210095
  • Received:2012-05-05 Online:2012-09-15 Published:2012-07-12

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Abstract: 【Objective】Genetic presentation of BC1 between ‘Zhongshanjingui’ and ‘Zhongshanjingui’×Ajania przewalskii. was studied in order to obtain new germplasm with higher ornamental and cold tolerance.【Method】Backcross hybridizations were made between Dendranthema morifolium variety ‘Zhongshanjingui’ (as female) and ‘Zhongshanjingui’×Ajania przewalskii (as male). The backcross hybrids were obtained and their hybridity was confirmed by a combination of morphological, cytological analysis and resistance comparison.【Result】A total of 17 backcross hybrids were obtained. The hybrids differentiated obviously from their parents in many morphological characters and all hybrid lines bloomed normally. There happened three flower types, i.e. standard anemone type, mid-anemone type and non-anemone type, and most were anemone type. Some hybeids’ inflorescence diameter was greater than ‘Zhongshanjingui’, the flower color was the same as ‘Zhongshanjingui’. Compared with the male parent, the ornamental value was greatly improved. The chromosome number of ‘Zhongshanjingui’ and ‘Zhongshanjingui’×Ajania przewalskii were 54 and 45, respectively. Their backcross hybrids chromosome number was between 41-54. In comparison of cold resistance, almost all the hybrids showed higher tolerance than ‘Zhongshanjingui’, which illustrated the backcross hybrids preserved the cold tolerance of Ajania przewalskii. 【Conclusion】By using backcross between intergeneric hybrid F1 and its chrysanthemum parent, not only the intergeneric hybrid’s ornamental vatue can be improved, but new germplasm of chrysanthemum also can be created.

Key words: Chrysanthemum×morifolium, Ajania przewalskii, intergeneric hybridization, backcross, ornamental, cold tolerance, genetic

[1]李鸿渐. 中国菊花. 南京: 江苏科学技术出版社, 1993.

Li H J. Chrysanthemums in China. Nanjing: Jiangsu Scientific and Technical Press, 1993. (in Chinese)

[2]李鸿渐, 邵建文. 中国菊花品种资源的调查收集与分类. 南京农业大学学报, 1990, 13(1): 139-143.

Li H J, Shao J W. Investigation, collection and classification of chrysanthemum cultivars in China. Journal of Nanjing Agricultural University, 1990, 13(1): 139-143. (in Chinese)

[3]孟金陵. 植物生殖遗传学. 北京: 科学出版社, 1997.

Meng J L. Plant Genetics. Beijing: Scientific Press, 1997. (in Chinese)

[4]陈发棣, 陈佩度, 房伟民, 李鸿渐. 栽培小菊与野生菊间杂交一代的细胞遗传学初步研究. 园艺学报, 1998, 25(3): 308-309.

Chen F D, Chen P D, Fang W M, Li H J. Cytogenetics of F1-hybrids between two small-headed cultivars of Dendranthema × Grandiflorum and two wild Dendranthema species. Acta Horticulture Sinica, 1998, 25(3): 308-309. (in Chinese)

[5]李辛雷, 陈发棣. 菊属野生种、栽培菊花及种间杂种的RAPD分析. 南京农业大学学报, 2004, 27(3): 30-36.

Li X L, Chen F D. RAPD analysis of wild species, cultivars and interspecific hybrids in Dendranthema. Journal of Nanjing Agricultural University, 2004, 27(3): 30-36. (in Chinese)

[6]石  铸, 傅国勋. 中国植物志第七十六卷第一分册. 北京: 科学出版社, 1983.

Shi Z, Fu G X. Flora Republicae Popularis Sinicae, 76(1). Beijing: Scientific Press, 1983. (in Chinese)

[7]李  玮. 广义菊属远缘杂交的研究进展. 北京: 北京林业大学, 2006.

Li W. Preliminary studies on intergeneric hybridization with in chrysanthemum in broad sense. Beijing: Beijing Forestry University, 2006. (in Chinese)

[8]尹佳蕾. 菊亚族部分属间远缘杂交的初步研究. 北京: 北京林业大学, 2005.

Yin J L. Preliminary studies on intergeneric hybridization in Asteraceae-Anthemideae Chrysantheminae tribe. Beijing: Beijing Forestry University, 2005. (in Chinese)

[9]Watson L E, Bates P L, Evans T M, Unwin M M, Estes J R. Molecular phylogeny of subtribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera. BMC Evolutionary Biology, 2002(2): 17-28.

[10]Tanaka R, Watanable K. Embryological studies in Chrysanthemum makinoi and its hybrid crossed with hexaploid Ch. Japenense. Journal of Science of the Hiroshima University, Series B Div. 2(Botany), 1994, 14(2): 75-84.

[11]Chen S M, Chen F D, Cheng X, Zhang F. The embryo rescue derived intergeneric hybrid between chrysanthemum and Ajania przewalskii Shows enhanced cold tolerance. Plant Cell Reports, 2011(30): 2177-2186.

[12]Douzono M, Ikeda H. All year round productivity of F1 and BC1 progenies between Dendranthema grandiflorum and D. shiwogik. Acta Horticulturae, 1998, 454: 303-310.

[13]Abd El-Twab MH, Kondo K, Hong DY. Isolation of a particular chromosome of Ajania remotipinna in a chromosome complement of an artificial F1 hybrid of Dendranthema lavandulifolia × Ajania remotipinna by use of genomic in situ hybridization. Chromosome Science, 1999(3): 21-28.

[14]赵宏波, 陈发棣, 缪恒彬, 郭维明. 栽培菊花与矶菊属间杂种亲和性及F1结实特性研究. 广西植物, 2009, 29(2): 171-175.

Zhao H B, Chen F D, Miao H B, Guo W M. Intergeneric cross-compatibility between Dendranthema × grandiflorum and Ajania pacifica and the seed sets of their F1 progenies in different conditions of backcross, selfing and open pollination. Guihaia, 2009, 29(2): 171-175. (in Chinese)

[15]邓衍明. 利用属间远缘杂交创造栽培菊花抗逆新种质的研究[D]. 南京: 南京农业大学, 2010.

Deng Y M. Study on chrysanthemum resistant germplasm innovation by intergeneric hybridization[D]. Nanjing: Nanjing Agricultural University, 2010. (in Chinese)

[16]Zhao H E, Liu Z H, Hu X, Yin J L, Li W, Rao G Y, Zhang X H, Huang C L, Anderson N, Zhang Q X, Chen J Y. Chrysanthemum genetic resources and related genera of Chrysanthemum collected in China. Genetic Resource and Crop Evolution, 2009, 56(7): 937-946.

[17]汤访评. 菊属与四个近缘属植物远缘杂交研究[D]. 南京: 南京农业大学, 2009.

Tang F P. Intergeneric hybridization between Dendranthema and four allied genera[D]. Nanjing: Nanjing Agricultural University, 2009. (in Chinese)

[18]Chen F D, Li F T, Chen S M, Guan Z Y, Fang W M. Meiosis and pollen germinability in small-flowered anemone type chrysanthemum cultivars. Plant Systematics and Evolution, 2009, 280: 143-151.

[19]Anderson N, Gesick E. Phenotypic markers for selection of winter hardy garden chrysanthemum (Dendranthema × grandiflora Tzvelv.) genotypes. Scientia Horticulturae, 2004, 101: 153-167.

[20]李合生, 孙  群, 赵世杰, 章文华. 植物生理生化实验原理和技术. 北京:高等教育出版社, 2000.

Li H S, Sun Q, Zhao S J, Zhang W H. Experimental Principle and Technique for Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000. (in Chinese)

[21]Anderson N. Chrysanthemum, Chrysanthemum × grandiflora Tzvelv// Anderson N. ed. Flower Breeding and Genetics Issues, Challenges and Opportunities for the 21st Century. Berlin: Springer, 2007: 389-437.

[22]李立会, 杨欣明, 李秀全, 董玉琛, 陈学明. 通过属间杂交向小麦转移冰草优异基因的研究. 中国农业科学, 1998, 31(6): 1-5.

Li L H, Yang X M, Li X Q, Dong Y C, Chen X M. Introduction of desirable genes from Agropyron cristatum into common wheat by intergeneric hybridization. Scientia Agricultura Sinica, 1998, 31(6): 1-5. (in Chinese)

[23]赵宏波, 陈发棣, 房伟民, 郭维明, 谢  伟. 利用亚菊属矶菊获得栽培菊花新种质. 中国农业科学, 2008, 41(7): 2077-2084.

Zhao H B, Chen F D, Fang W M, Guo W M, Xie W. Creating novel germplasms of chrysanthemum by employing the Ajania pacifica. Scientia Agricultura Sinica, 2008, 41(7): 2077-2084. (in Chinese)

[24]Anderson N, Gesick E. Phenotypic markers for selection of winter hardy garden chrysanthemum (Dendranthema × grandiflora Tzvelv.) genotypes. Scientia Horticulturae, 2004, 101: 153-167.
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