Scientia Agricultura Sinica

Previous Articles     Next Articles

Correlations Between Changes of Absorption and Transformation of Light Energy by PSII in Pepper Leaves and the Variety Tolerance Under Low Temperature and Weak Light

XIE Jian-ming, YU Ji-hua, HUANG Gao-bao, FENG Zhi   

  1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070
  • Received:2010-11-30 Online:2011-05-05 Published:2011-02-23

PDF

557

Cited

12

Abstract: 【Objective】 The objective of this study is to explore the correlations between the changes of absorption and transformation of light energy by PSII in pepper leaves and the variety tolerance under low temperature and weak light, and to screen suitable indexes for evaluating chilling- and weak light-tolerance of pepper varieties.【Method】 The correlations between the changes of chlorophyll fluorescence parameters and the tolerance of 12 pepper varieties expressed as injury indexes under low temperature and weak light were studied by using modulated chlorophyll fluorescence techniques and correlation analysis.【Result】 After treated with low temperature (15℃/5℃, day/night) and weak light (100 μmol·m-2·s-1), the ΦPSⅡ(actual photochemical efficiency of PSⅡ), Fv′/Fm′ (photochemical efficiency of open reaction centers of PSⅡ), qP (photochemical quenching), Fm′ (maximal fluorescence in light-adapted state), Fo′ (minimal fluorescence in light-adapted state) and Fv′(variable fluorescence in light-adapted state) in leaves of 12 pepper varieties decreased, NPQ (non-photochemical quenching) and Fs (steady-state fluorescence) increased, wherease the Fv/Fm changed little in leaves of 12 pepper varieties. Significant negative correlations between chilling injury indexes and ΦPSⅡ, Fv′/Fm′, qP, Fm′ or Fv′ were observed in 12 pepper variety leaves treated with low temperature and weak light for 15 days；wherease a significant positive correlation between chilling injury indexes and NPQ was observed in pepper leaves treated with low temperature and weak light conditions for 15 days.【Conclusion】 It was proposed that ΦPSⅡ, Fv′/Fm′, qP, NPQ, Fm′ and Fv′ in leaves of pepper treated with 15°C/5°C, 100 μmol·m-2·s-1PFD for 15 days could be used as simple and convenient tools for identification and screening a large number of chilling and weak-light tolerant pepper varieties. Among these parameters, ΦPSⅡ and Fv′ were the most sensitive ones.

Key words: pepper , low temperature and weak light , chlorophyll fluorescence , chilling- and weak-light-tolerance

[1]陈青君. 黄瓜低温弱光耐受性评价指标体系以及弱光耐受性QTL定位的研究//中国农业科学院博士后流动站博士后研究工作报告. 北京: 中国农业科学院, 2003.
Chen Q J. Study on identification index system of low temperature and poor light adaptability of cucumber and mapping QTL controlling poor light adaptability. Post-doctoral research report of mobile post-doctoral research station of Chinese Academy of Agricultural Sciences. Beijing: Chinese Academy of Agricultural Sciences, 2003. (in Chinese)
[2]张志刚, 尚庆茂. 低温、弱光及盐胁迫下辣椒叶片的光合特性.中国农业科学, 2010, 43(1): 123-131.
Zhang Z G, Shang Q M. Photosynthetic characteristics of pepper leaves under low temperature, weak light and salt stress. Scientia Agricultura Sinica, 2010, 43(1):123-131. (in Chinese)
[3]Wery J, Silim S N, Knights E J, Malhotra R S, Cousin R. Screening techniques and sources of tolerance to extremes of moisture and air temperature in cool season food legumes. Euphytica, 1994, 73: 73-83.
[4]Rohá?ek K. Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning, and mutual relationships. Photosynthetica, 2002, 40(1): 13-29.
[5]Sayed O H. Chlorophyll fluorescence as a tool in cereal crop research. Photosynthetica, 2003, 41(3): 321-330.
[6]Schreiber U, Schliwa U, Bilger W. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynthesis Research, 1986, 10: 51-62.
[7]Sthapit B R, Witcombe J R, Wilson J M. Methods of selection for chilling tolerance in Nepalese rice by chlorophyll fluorescence analysis. Crop Science, 1995, 35: 90-94.
[8]Bertin P, Kinet J M, Bouharmont J. Evaluation of chilling sensitivity in different rice varieties. Relationship between screening procedures applied during germination and vegetative growth. Euphytica, 1996, 89: 201-210.
[9]高青海, 徐 坤, 高辉远, 吴 燕. 不同茄子砧木幼苗抗冷性的筛选. 中国农业科学, 2005, 38(5): 1005-1010.
Gao Q H, Xu K, Gao H Y, Wu Y. Screening on chilling tolerance of different eggplant rootstock seedlings. Scientia Agricultura Sinica, 2005, 38(5): 1005-1010. (in Chinese)
[10]王洪涛, 艾希珍, 秦  竞, 王美玲. 几个甜椒砧木品种对低温弱光耐受性的差异. 西北农业学报, 2008,17(1): 233-237
Wang H T, Ai X Z, Qin J, Wang M L. Difference of tolerance to low temperature and light intensity in five rootstock varieties of sweet pepper. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(1): 233-237. (in Chinese)
[11]刘学庆, 王秀峰, 朴永吉. 蝴蝶兰不同品种耐冷特性的研究.园艺学报, 2007, 34(2): 425-430.
Liu X Q, Wang X F, Pu Y J. A study on cold tolerance of different phalaenopsis cultivars. Acta Horticulturae Sinica, 2007, 34(2): 425-430. (in Chinese)
[12]樊志成, 贾洪玉, 郭洪芸, 杨 梁. 西葫芦耐冷性生理指标研究. 园艺学报, 1999,26(5):309-313.
Fan Z C, Jia H Y, Guo H Y, Yang L. Studies on the physiological criteria for cold tolerance evaluation in Cucurbita pepo L. Acta Horticulturae Sinica, 1999, 26(5): 309-313. (in Chinese)
[13]Haldimann P. Chilling-induced changes to carotenoid composition, photosynthesis and maximum quantum yield of photosystem Ⅱ photochemistry in two maize genotypes differing in tolerance to low temperature. Journal of Plant Physiology, 1997, 151: 610-619.
[14]Frachebound Y, Haldimnn P, Leipner J, Stamp P. Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.). Journal of Experimental Botany, 1999, 50(338): 1533-1540.
[15]Strauss A J, Krüger G H J, Strasser R J, Van Heerden P D R. Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O-J-I-P. Environmental and Experimental Botany, 2006, 56: 147-157.
[16]Havaux M. Fluorimetric determination of the genetic variability existing for chilling tolerance in sweet sorghum and Sudan grass. Plant Breeding, 1989, 102: 327-332.
[17]Ko?cielniak J, Biesaga-Ko?cielniak J. Photosynthesis and non- photochemical excitation quenching components of chlorophyll excitation in maize and field bean during chilling at different photon flux density. Photosynthetica, 2006, 44(2): 174-180.
[18]Liu P, Meng Q W, Zhao S J, Liu Q Z. Effects of cold-hardening on chilling-induced photoinhibition of photosynthesis and on xanthophyll cycle pigments in sweet pepper. Photosynthetica, 2001, 39(3): 467-472.
[19]Li X G, Wang X M, Meng Q W, Zou Q. Factors limiting photosynthetic recovery in sweet peper leaves after short-term chilling stress under low irradiance. Photosynthetica, 2004, 42(2): 257-262.
[20]张国斌, 郁继华. 低温弱光对辣椒幼苗光合特性与光合作用启动时间的关系. 西北植物学报, 2006, 26(9): 1770-1775.
Zhang G B, Yu J H. Effects of low temperature on photosynthetic characteristics and starting time of pepper seedlings under weak  light. Acta Agriculturae Boreali-occidentalis Sinica, 2006, 26(9): 1770-1775. (in Chinese).
[21]刘慧英, 王祯丽, 王玉华. 不同品种辣椒种子发芽和苗期耐冷性差异的研究. 石河子大学学报: 自然科学版, 2002, 6(1): 23-26.
Liu H Y, Wang Z L, Wang Y H. Studies on the chilling tolerance of different pepper varieties during germination of seeds and seeding. Journal of Shihezi University: Natural Science, 2002, 6(1): 23-26. (in Chinese).
[22]Bilger W, Björkman O. Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in Hedera canariensis. Photosynthesis Research, 1990, 25: 173-185.
[23]Haldimnn P, Frachebound Y, Stamp P. Photosynthetic performance and resistance to photoinhibition of Zea mays L. leaves grown at sub-optimal temperature. Plant, Cell and Environment, 1996, 19: 85-92.
[24]Yu J Q, Zhou Y H, Huang L F, Allen D J. Chill-induced inhibition of photosynthesis: Genotypic variation within Cucumis sativus. Plant and Cell Physiology, 2002, 43(10): 1182-1188.
[25]Demmig-Adams B, Winter K, Krüger A, Czygan F C. Zeaxanthin synthesis, energy dissipation, and photoprotection of photosystem Ⅱ at chilling temperatures. Plant Physiology, 1989, 90: 894-898.
[26]Wang G L, Guo Z F. Effects of chilling stress on photosynthetic rate and chlorophyll fluorescence parameters in seedlings of two rice cultivars differing in cold tolerance. Rice Science, 2005, 12(3): 187-191.
[27]Lin K H, Hwang W C, Lo H F. Chilling stress and chilling tolerance of sweet potato as sensed by chlorophyll fluorescence. Photosynthetica, 2007, 45(4): 628-632.
[28]Jung S, Steffen K L, Lee H J. Comparative photoinhibition of a high and a low altitude ecotype of tomato (Lycopersicon hirsutum) to chilling stress under high and low light conditions. Plant Science, 1998, 134: 69-77.
[29]李志博, 魏亦农, 杨  敏, 张荣华.低温胁迫对棉花幼苗叶绿素荧光特性的影响初探. 棉花学报, 2006, 18(4): 255.
Li Z B, Wei Y N, Yang M, Zhang R H. Primary study on effects of low temperature on chlorophyll fluorescence characteristics of cotton seedling. Cotton Science, 2006, 18(4): 255. (in Chinese)
[30]周艳虹, 黄黎峰, 喻景权. 持续低温弱光对黄瓜叶片气体交换、叶绿素荧光猝灭和吸收光能分配的影响. 植物生理与分子生物学学报, 2004, 30(2): 153-160.
Zhou Y H, Huang L F, Yu J Q. Effects of sustained chilling and low light on gas exchange, chlorophyll fluorescence quenching and absorbed light allocation in cucumber leaves. Journal of Plant Physiology and Molecular Biology, 2004, 30(2): 153-160. (in Chinese)
[31]胡文海, 喻景权. 低温弱光对番茄叶片光合作用和叶绿素光化学效率的影响. 园艺学报,  2001, 28(1): 41-46.
Hu W H, Yu J Q. Effects of chilling under low light on photosynthesis and chlorophyll fluorescence characteristic in tomato leaves. Acta Horticulturae Sinica, 2001, 28(1): 41-46. (in Chinese)
[1] NING RuoYun, YIN YuQi, SHEN JianGuo, ZHANG ShuLing, GONG MeiFang, GAO FangLuan. The Phylogeographic History of Pepper Mild Mottle Virus [J]. Scientia Agricultura Sinica, 2026, 59(3): 543-555.
[2] WANG MengYuan, WEI QianRui, LI HaiYan, YANG QiaoMin, YU Jun, HUANG Wei, LU MingHui. Functional Analysis of MADS-box Transcription Factor Gene CaAGL61 in Heat Tolerance of Pepper [J]. Scientia Agricultura Sinica, 2025, 58(8): 1604-1616.
[3] ZHENG Yu, CHEN Yi, TI JinSong, SHI LongFei, XU XiaoBo, LI YuLin, GUO Rui. Evaluation of Carbon Footprint and Economic Benefit of Different Tobacco Rotation Patterns [J]. Scientia Agricultura Sinica, 2025, 58(4): 733-747.
[4] LI NiFei, YANG QiaoMin, YANG KeCheng, XING YuTeng, WANG MengYuan, ZANG TianBao, LU MingHui. Functional Analysis of CaIAA8, An Interacting Protein of the Autophagy-Related Protein CaATG8c, in the Heat Tolerance of Pepper [J]. Scientia Agricultura Sinica, 2025, 58(22): 4732-4745.
[5] LIU Jing, WANG Hong, ZHANG Lei, XIAO JiuJun, WU JianGao, GONG MingChong. Inversion of Nitrogen Content in Chili Pepper Leaves Based on Hyperspectral Analysis [J]. Scientia Agricultura Sinica, 2025, 58(2): 252-265.
[6] ZHANG Jie, HU ChenXi, QI JianBo, ZHANG YongTai, CHEN YiBo, ZHANG YongJi. Effects of Exogenous Zeatin on Photosynthetic Parameter, Antioxidant System and Expression of Genes Related to Zeatin Synthesis in Pepper Under Low-Temperature Combined with Low-Light Stress [J]. Scientia Agricultura Sinica, 2025, 58(19): 3959-3969.
[7] WU HuiQin, WANG Jing, YANG Yi, LIU XueQing, ZHANG KaiXuan, WANG LuYao, LU JiaWei, ZHAI Yuan, CHENG Yan. Analysis and Evaluation of Fruit Texture Quality of 96 Pepper Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(14): 2854-2868.
[8] XU JiaXin, HUA Nan, WANG YongQiang, XU Hao, LIU Zhen, ZHAO XiaoRui, LI Yue, CHEN QiWei, YE Lin. Response Surface Methodology Optimization of Water, Fertilizer, and Pesticide Coupling on Chili Pepper Growth, Photosynthetic Characteristics, and Root Rot [J]. Scientia Agricultura Sinica, 2025, 58(14): 2869-2884.
[9] MA Jia, PENG JieLi, JIA Nan, WANG Xu, WANG ZhanWu, HU Dong. Effects of Streptomyces sp. TOR3209 on Chlorophyll Fluorescence Characteristics and Xanthophyll Cycle in Tomato Plants Under Cold Stress [J]. Scientia Agricultura Sinica, 2024, 57(22): 4522-4540.
[10] LU Lu, LI YiNuo, ZHANG XiuGuo, GAO DanMei, WU FengZhi. Effects of Wheat and Broad Bean Catch Crop and Their Decomposed Liquids on the Growth and Blight of Continuous Cropping Pepper Seedlings [J]. Scientia Agricultura Sinica, 2024, 57(22): 4541-4552.
[11] HE Yong, FAN XiaoZhu, CHEN XinYue, DUAN ShuJing, HU TingTing, XIE RuXue, WANG YuQing, CHEN Jing. Screening and Verification of Pepper Host Factors Interacting with the 126 kDa Protein of Pepper Mild Mottle Virus by Yeast Two-Hybrid System [J]. Scientia Agricultura Sinica, 2024, 57(15): 2986-2996.
[12] GAO ChengAn, WAN HongJian, YE QingJing, CHENG Yuan, LIU ChenXu, HE Yong. Identification and Comparative Analysis of Processed/Fresh-Eating Chili Pepper Fruits at Different Maturation Stages by Metabolomics [J]. Scientia Agricultura Sinica, 2024, 57(12): 2424-2438.
[13] ZHOU DaoMing, SUN Tao, ZHAO YuHong, JIA YuanJie, YANG MingFei, QU Feng, HU XiaoHui. Optimization of Water and Fertilizer Management of Substrate Cultivated Peppers Based on Quality, Yield, and Water and Fertilizer Use Efficiency [J]. Scientia Agricultura Sinica, 2023, 56(12): 2354-2366.
[14] DONG SangJie,JIANG XiaoChun,WANG LingYu,LIN Rui,QI ZhenYu,YU JingQuan,ZHOU YanHong. Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(6): 1189-1198.
[15] HU XueHua,LIU NingNing,TAO HuiMin,PENG KeJia,XIA Xiaojian,HU WenHai. Effects of Chilling on Chlorophyll Fluorescence Imaging Characteristics of Leaves with Different Leaf Ages in Tomato Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(24): 4969-4980.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd