Abstract:

【Objective】 Polymorphism of heart fatty acid-binding protein (H-FABP) gene and its correlation with IMF content in Wei pig were studied. 【Method】 PCR-RFLP (HinfⅠ, HaeⅢ, MspⅠ3 kinds of restriction enzymes) and molecular marker techniques were used in the study and the genetic variation of H-FABP gene 5'-upstream region and second intron in 123 Wei pigs was analyzed. 【Result】 In 5'-upstream region, there was polymorphism in loci of the HinfⅠand the frequency of allele H was 0.6545, showing moderate polymorphism (PIC = 0.3500). In the second intron, polymorphism was detected in polymorphic loci HinfⅠ*, the frequency of allele B was 0.7195, showing moderate polymorphism (PIC = 0.3222). Loci of HaeⅢ also existed, allele D frequency was 0.6301, showing moderate polymorphism (PIC = 0.3575 ). While no polymorphism was detected in the loci of MspⅠ, which the genotype was AA genotype. The gene frequencies and genotype frequencies of pigs tested have reached the Hardy-Weinberg equilibrium (P＞0.05). The detection of the genotype of the IMF content had the trend of HH＞Hh＞hh, dd＞Dd＞DD, BB＞Bb＞bb, genetic effect values were as following: 4.6918, 4.2665, 3.7712, 4.6124, 4.3167, 3.8173, 4.3042, 4.2358, and 4.1970. Cloning and sequencing analysis on polymorphic fragments of three PCR-RFLP in H-FABP gene showed that HinfⅠ-RFLP is due to T→C mutation in 1 324 bp, HaeⅢ-RFLP is due to C→G mutation in 1 811 bp, HinfⅠ*- RFLP is due to T→C mutation in 1 970 bp. Sequencing results had 97.9% and 98.3% homology with the sequence X98558 and Y16180 published by GenBank. 【Conclusion】Polymorphisms in 5'-upstream region and the second intron of H-FABP gene had certain effect on IMF content in Wei pig. However, whether these polymorphisms are important genetic markers, which are related with H-FABP gene and IMF content in Wei pig, are needs to be further studied.

Key words: Wei pig, H-FABP gene, IMF content, sequence analysis, PCR-RFLP