Abstract:

【Objective】The anticarcinogenic activity of broccoli is most likely to be due to the activity of the isothiocyanates sulforaphane derived from glucoraphanin. In order to provide information in broccoli breeding for high nutrition quality, the goal of this study is to determine the contents of glucoraphanin and sulforaphane and analyze the correlation between them which is in the florets, stems and leaves of different genotypes of broccoli.【Result】Both glucoraphanin and sulforapane content difference was extremely significant in the florets, stems and leaves of different broccoli genotypes. The highest contents of them were both in the florets, while the lowest contents were in the leaves. The mean value of glucoraphanin content in the florets was 4.4- and 13.97-fold, respectively, higher than that in the stems and leaves, and the mean value of sulforaphane content in the florets was 4- and 8.7-fold, respectively, higher than that in the stems and leaves. However, compared to most materials, there were higher glucoraphanin contents in the stems and leaves of some broccoli materials about 50% of which in the florets. The correlation coefficient between the glucoraphanin and sulforaphane contents in the florets, stems and leaves of the ten different genotype broccoli materials were 0.9886**, 0.9994**, 0.9935**, respectively.【Conclusion】There exist some genotypic differences which can significant affect the glucoraphanin content in different genotype broccoli materials and organs. In the development and application of sulforaphane, the glucoraphanin contents can be the screening index. Broccoli materials which have higher glucoraphanin contents in the stems and leaves can be selected as the raw materials for extraction of the sulforaphane from the wastes.

Key words: broccoli, glucoraphanin, sulforaphane