Abstract:

【Objective】The purpose of this experiment was to provide a theoretical basis for selective breeding of the low phytic acid maize hybrid through screening low phytic acid materials from general maize inbred lines and study the genetic characteristics of low phytic acid trait. 【Method】In this study, through using the colorinmetric assay for high inorganic phosphorus (HIP), kernels from 100 maize inbred lines were indirectly screened respectively. To analyze the genetic characteristics of low-phytic acid trait, the screened Luyuan 92 was hybridized with known low phytic acid mutants Lpa/lpa241, Lpa/Lpa241 and low-phytic acid inbred line Qi 319. Also the phytic acid content of two inbred lines Qi 319, Luyuan 92 and their reciprocal hybrids from six different origins were analysed by modified colorimetric assay and GB technique. The phenotypes of Qi 319, Luyuan 92×Qi 319, Qi 319×Lpa241/lpa241, Luyuan 92×Lpa241/lpa241 seedlings were investigated through germination test.【Result】 The inorganic phosphorus content of Luyuan 92 was about 0.93 μg?mg-1, which was higher than that of other materials. According to the screening results of F1 seeds between mutants (L/lpa241) and Qi 319, Luyuan 92, the segregation ratio of HIP type and wild type fit into 1﹕1 respectively, which indicated that its low phytic acid character was controlled by single recessive gene, and was possibly allelic with lpa241. The phytic acid content of the same inbred line from six different origins displayed a significant difference. Qi 319 represented heterozygosity in low phytic acid character and lethal recessive homozygous genotype at seedling stage. 【Conclusion】In this study, one maize inbred line with low phytic acid content was obtained. Its low phytic acid trait was predicted preliminarily to be controlled by single recessive gene and was possibly allelic with lpa241.

Key words: maize (Zea mays L.), inbred line, low phytic acid (lpa), genetics