Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (21): 4377-4384.doi: 10.3864/j.issn.0578-1752.2011.21.006

• PLANT PROTECTION • Previous Articles     Next Articles

Changes of mRNA Expression of Five Defense Genes of Cotton Response to Damages Caused by Different Phytophagous Insects

 LI  Jing, ZHANG  Shuai, CUI  Jin-Jie   

  1. 1.中国农业科学院棉花研究所，河南安阳 455000
  • Received:2011-03-04 Online:2011-11-01 Published:2011-05-31

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Abstract: 【Objective】The objective of this study is to understand the role of 5 defense protein genes defensing the phytophagous pests in cotton.【Method】Using the real-time quantitative PCR, the expression changes of the polyphenol oxidase (PPO), chloroplast Cu/Zn superoxide dismutase, ATP synthase β subunit, light-harvesting protein complex Ⅱ and heat shock protein (Hsp70) genes, which were damaged by Helicoverpa armigera, Spodoptera litura, Spodoptera exigua and mechanical damage for 0 (control), 6, 12, 24, 48, 60 h, respectively, were studied. 【Result】 In the 4 damage treatments, the polyphenol oxidase, heat shock protein and chloroplast Cu/Zn superoxide were stronger in response to insect feeding damage, but the light-harvesting protein complex Ⅱ was more sensitive to the mechanical damage, and ATP synthase β subunit was stronger in response to the insect feeding damage and mechanical damage. PPO responded rapidly to the H. armigera infestation, while the reaction to the S. exigua infestation was rather slowly, and without specific answer to the damage of S. litura. After 4 treatments, Hsp70 expression had a significant change, and it increased larger against the damages caused by H. armigera and S. exigua. ATP synthase β subunit was down-regulated to mechanical damage and S. litura. While after damage caused by H. armigera and S. exigua the expression was up-regulated. The light-harvesting protein complex Ⅱ was down-regulated except the damage caused by H. armigera. Chloroplast Cu/Zn superoxide was down-regulated when damaged by machine and S. litura but it up-regulated to the H. armigera and S. exigua, and it responded stronger to the damage caused by H. armigera than S. exigua.【Conclusion】The response of the cotton to different insect damages was different and with a certain specificity.

Key words: cotton, defense gene, induced responses, quantitative

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