Abstract: 【Objective】 The characterization of protein expression at different developmental stages may help to understand the forming mechanisms of elite varieties and then promote their applications in breeding.【Method】In this study, Western blotting were used to investigate the expression of nine proteins, in the categories of photosynthesis, reactive oxygen scavenging, and defense/stress responses, among six rice elite varieties including 9311, PA64s, Teqing, Zhenshan 97B, Guangluai 4 and Aijiaonante, at different developmental stages/tissues including the shoot and root at seedling stage, the flag leaf and panicle at flowering stage, the flag leaf and panicle at filling stage.【Result】The results indicated that polymorphisms of protein expression presented among different rice varieties, the expression patterns in root and panicle varied to a large extent, while the expression in leaves was similar among different rice varieties. Additionally, the polymorphisms also presented among different developmental stages/tissues, indicating the temporal and spatials expressions of these proteins.【Conclusion】This study has established a strategy to investigate the expression of rice proteins by using antibodies.

Key words: rice (Oryza sativa L.), elite variety, protein, polymorphism