Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (9): 2073-2078 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Development and application of nested PCR assay for detection of dairy cattle-derived Cyclospora sp.

  

  1. 华南农业大学兽医学院
  • Received:2006-05-22 Revised:2007-01-10 Online:2007-09-10 Published:2007-09-10

PDF

767

Abstract: Based on the cattle-derived Cyclospora sp. sequences, two pairs of primers were designed. These primers selectively amplified a 168-bp DNA fragment of the 18S rRNA gene of cattle-derived Cyclospora sp. With these primers, a nested PCR assay for detection of cattle-derived Cyclospora sp. was developed. The results showed that the nested PCR assay was specific and there is no cross-reaction with other parasites, such as Eimeria spp., Cryptosporidium spp., Giardia sp., Toxoplasma sp., Trichuris sp. and cattle ciliate. The assay was able to detect as low as 2.85×10-2 fg of control positive DNA. The results of detection of clinical samples revealed that the assay was coinciding with microscopic examination and it will be an effective tool for detection of Cyclospora sp. in cattle feces.

Key words: Cyclospora sp., Dairy cattle, Nested PCR, Detection

[1] LI ZhiLing,LI XiangJu,CUI HaiLan,YU HaiYan,CHEN JingChao. Development and Application of ELISA Kit for Detection of EPSPS in Eleusine indica [J]. Scientia Agricultura Sinica, 2022, 55(24): 4851-4862.
[2] XIE LiXue,ZHANG XiaoYan,ZHANG LiJie,ZHENG Shan,LI Tao. Complete Genome Sequence Characteristics and TC-RT-PCR Detection of East Asian Passiflora Virus Infecting Passiflora edulis [J]. Scientia Agricultura Sinica, 2022, 55(22): 4408-4418.
[3] CUI JiangKuan,REN HaoHao,CAO MengYuan,CHEN KunYuan,ZHOU Bo,JIANG ShiJun,TANG JiHua. SCAR-PCR Rapid Molecular Detection Technology of Heterodera zeae [J]. Scientia Agricultura Sinica, 2022, 55(17): 3334-3342.
[4] ZHANG JingYuan,MIAO FaMing,CHEN Teng,LI Min,HU RongLiang. Development and Application of a Real-Time Fluorescent RPA Diagnostic Assay for African Swine Fever [J]. Scientia Agricultura Sinica, 2022, 55(1): 197-207.
[5] LI ZhenXi,LI WenTing,HUANG JiaQuan,ZHENG Zheng,XU MeiRong,DENG XiaoLing. Detection of ‘Candidatus Liberibacter asiaticus’ by Membrane Adsorption Method Combined with Visual Loop-Mediated Isothermal Amplification [J]. Scientia Agricultura Sinica, 2022, 55(1): 74-84.
[6] DUAN Yu,XU JianJian,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen. Detection of Citrus Leaf Blotch Virus by Reverse Transcription- Recombinase Polymerase Amplification (RT-RPA) [J]. Scientia Agricultura Sinica, 2021, 54(9): 1904-1912.
[7] Xue BAI,Teng HUI,ZhenYu WANG,YunGang CAO,DeQuan ZHANG. Determination of 5 Nitropolycyclic Aromatic Hydrocarbons in Roasted Meat Products by High Performance Liquid Chromatography- Fluorescence Detection [J]. Scientia Agricultura Sinica, 2021, 54(5): 1055-1062.
[8] Tao WANG,Yu HAN,Li PAN,Bing WANG,MaoWen SUN,Yi WANG,YuZi LUO,HuaJi QIU,Yuan SUN. Development of a TaqMan Real-Time PCR Targeting the MGF360-13L Gene of African Swine Fever Virus [J]. Scientia Agricultura Sinica, 2021, 54(5): 1073-1080.
[9] JiaJia LI,HuiLong HONG,MingYue WAN,Li CHU,JingHui ZHAO,MingHua WANG,ZhiPeng XU,Yin ZHANG,ZhiPing HUANG,WenMing ZHANG,XiaoBo WANG,LiJuan QIU. Construction and Application of Detection Model for the Chemical Composition Content of Soybean Stem Based on Near Infrared Spectroscopy [J]. Scientia Agricultura Sinica, 2021, 54(5): 887-900.
[10] MA ZhiMin,XU JianJian,DUAN Yu,WANG ChunQing,SU Yue,ZHANG Qi,BIN Yu,ZHOU ChangYong,SONG Zhen. Establishment of RT-RPA for Citrus Yellow Vein Clearing Virus (CYVCV) Detection [J]. Scientia Agricultura Sinica, 2021, 54(15): 3241-3249.
[11] CHEN PengFei,MA Xiao. Research Status and Trends of Automatic Detection of Crop Planting Rows [J]. Scientia Agricultura Sinica, 2021, 54(13): 2737-2745.
[12] HUI YuanYuan,PENG HaiShuai,WANG BiNi,ZHANG FuXin,LIU YuFang,JIA Rong,REN Rong. Research Progress of Food-Borne Pathogen Detection Based on Electrochemical and Optical Aptasensors [J]. Scientia Agricultura Sinica, 2021, 54(11): 2419-2433.
[13] ZHANG QingAn,CHEN BoYu. Research Progress of Four Sulfur Compounds Related to Red Wine Flavor [J]. Scientia Agricultura Sinica, 2020, 53(5): 1029-1045.
[14] MoRan XU,RuiMing LIN,FengTao WANG,Jing FENG,ShiChang XU. Evaluation of Resistance to Stripe Rust and Genetic Diversity and Detection of Resistance Genes in 103 Wheat Cultivars (Lines) [J]. Scientia Agricultura Sinica, 2020, 53(4): 748-760.
[15] ZHANG HaiLiang,LIU AoXing,MI SiYuan,LI Xiang,LUO HanPeng,YAN XinYi,WANG YaChun. A Review on Longevity Trait in Dairy Cattle Breeding [J]. Scientia Agricultura Sinica, 2020, 53(19): 4070-4082.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd