Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (8): 1809-1816 .doi: 10.3864/j.issn.0578-1752.at-2006-7731

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Molecular Cloning, Analysis and Prokaryotic Expression of the Translationally Controlled Tumor Protein Gene in Bombyx mori

  

  1. 中国科学院上海生命科学研究院植物生理生态研究所
  • Received:2006-06-23 Revised:1900-01-01 Online:2007-08-10 Published:2007-08-10

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Abstract: In order to reveal the function of TCTP gene in Bombyx mori, we cloned the TCTP gene using 5’RACE depending on the tag get from SAGE and the result of GLGI. To compare the homology the TCTP in Bombyx mori with other species’, we constructed a primary phylogenic tree. On the other hand, we amplify TCTP gene from various stage or tissue of Bombyx mori to learn the expression of TCTP gene in the insect. Furthermore, to confirm the exact function of TCTP, we get the recombinant protein of it by E. coli expression system.

Key words: Bombyx mori, TCTP, tag, Homolog analysis, Phylogenetic tree

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