Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (12): 2688-2695 .doi: 10.3864/j.issn.0578-1752.at-2006-8328

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES • Previous Articles     Next Articles

Cloning and Sequence Analysis of Genes for Flavonoid Biosynthesis in Brassica juncea

  

  1. 湖南农业大学油料作物研究所
  • Received:2006-11-06 Revised:1900-01-01 Online:2007-12-10 Published:2007-12-10

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Abstract: 【Objective】 The genes for flavonoid biosynthesis are to be cloned in Brassica juncea .【Method】Homology-based cloning strategy was used in this study. The primers were designed according to the sequences of homologous genes of the model plant Arabidopsis thaliana. The gene fragments were sequenced, and analyzed by BLAST.【Result】The 17 fragments were cloned and sequenced from 13 pairs of specific primers, which were confirmed to correspond to the Arabidopsis thaliana homologous genes and belong to the 13 known functional genes. The gene chs for chalcone synthase has 3 fragments different in size, while the genes pap for production of anthocyanin pigment and chi for chalcone isomerase have 2 fragments each. 【Conclusion】The homology- based cloning of the genes for flavonoid biosynthesis in Brassica juncea is viable. The cloned fragments of genes for flavonoid biosynthesis will provide the foundations for uncovering the genetic regulation of seed coat color formation in Brassica juncea.

Key words: Brassica juncea, Flavonoid, Genes, Homology-based cloning

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