Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (12): 2688-2695 .

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES • Previous Articles     Next Articles

Cloning and Sequence Analysis of Genes for Flavonoid Biosynthesis in Brassica juncea

  

  1. 湖南农业大学油料作物研究所
  • Received:2006-11-06 Revised:1900-01-01 Online:2007-12-10 Published:2007-12-10

PDF

1049

Cited

2

Abstract: 【Objective】 The genes for flavonoid biosynthesis are to be cloned in Brassica juncea .【Method】Homology-based cloning strategy was used in this study. The primers were designed according to the sequences of homologous genes of the model plant Arabidopsis thaliana. The gene fragments were sequenced, and analyzed by BLAST.【Result】The 17 fragments were cloned and sequenced from 13 pairs of specific primers, which were confirmed to correspond to the Arabidopsis thaliana homologous genes and belong to the 13 known functional genes. The gene chs for chalcone synthase has 3 fragments different in size, while the genes pap for production of anthocyanin pigment and chi for chalcone isomerase have 2 fragments each. 【Conclusion】The homology- based cloning of the genes for flavonoid biosynthesis in Brassica juncea is viable. The cloned fragments of genes for flavonoid biosynthesis will provide the foundations for uncovering the genetic regulation of seed coat color formation in Brassica juncea.

Key words: Brassica juncea, Flavonoid, Genes, Homology-based cloning

[1] HU Sheng,LI YangYang,TANG ZhangLin,LI JiaNa,QU CunMin,LIU LieZhao. Genome-Wide Association Analysis of the Changes in Oil Content and Protein Content Under Drought Stress in Brassica napus L. [J]. Scientia Agricultura Sinica, 2023, 56(1): 17-30.
[2] LIU Jiao,LIU Chang,CHEN Jin,WANG MianZhi,XIONG WenGuang,ZENG ZhenLing. Distribution Characteristics of Prophage in Multidrug Resistant Escherichia coli as well as Its Induction and Isolation [J]. Scientia Agricultura Sinica, 2022, 55(7): 1469-1478.
[3] CHAO ChengSheng,WANG YuQian,SHEN XinJie,DAI Jing,GU ChiMing,LI YinShui,XIE LiHua,HU XiaoJia,QIN Lu,LIAO Xing. Characteristics of Efficient Nitrogen Uptake and Transport of Rapeseed at Seedling Stage [J]. Scientia Agricultura Sinica, 2022, 55(6): 1172-1188.
[4] WANG HuiLing, YAN AiLing, SUN Lei, ZHANG GuoJun, WANG XiaoYue, REN JianCheng, XU HaiYing. eQTL Analysis of Key Monoterpene Biosynthesis Genes in Table Grape [J]. Scientia Agricultura Sinica, 2022, 55(5): 977-990.
[5] SHU JingTing,SHAN YanJu,JI GaiGe,ZHANG Ming,TU YunJie,LIU YiFan,JU XiaoJun,SHENG ZhongWei,TANG YanFei,LI Hua,ZOU JianMin. Relationship Between Expression Levels of Guangxi Partridge Chicken m6A Methyltransferase Genes, Myofiber Types and Myogenic Differentiation [J]. Scientia Agricultura Sinica, 2022, 55(3): 589-601.
[6] TANG ZiYun,HU JianXin,CHEN Jin,LU YiXing,KONG LingLi,DIAO Lu,ZHANG FaFu,XIONG WenGuang,ZENG ZhenLing. Relationship Between Biofilm Formation and Molecular Typing of Staphylococcus aureus from Animal Origin [J]. Scientia Agricultura Sinica, 2022, 55(3): 602-612.
[7] LI Heng,ZI XiangDong,WANG Hui,XIONG Yan,LÜ MingJie,LIU Yu,JIANG XuDong. Screening of Key Regulatory Genes for Litter Size Trait Based on Whole Genome Re-Sequencing in Goats (Capra hircus) [J]. Scientia Agricultura Sinica, 2022, 55(23): 4753-4768.
[8] SUN BaoJuan,WANG Rui,SUN GuangWen,WANG YiKui,LI Tao,GONG Chao,HENG Zhou,YOU Qian,LI ZhiLiang. Transcriptome and Metabolome Integrated Analysis of Epistatic Genetics Effects on Eggplant Peel Color [J]. Scientia Agricultura Sinica, 2022, 55(20): 3997-4010.
[9] XIE XiaoYu, WANG KaiHong, QIN XiaoXiao, WANG CaiXiang, SHI ChunHui, NING XinZhu, YANG YongLin, QIN JiangHong, LI ChaoZhou, MA Qi, SU JunJi. Restricted Two-Stage Multi-Locus Genome-Wide Association Analysis and Candidate Gene Prediction of Boll Opening Rate in Upland Cotton [J]. Scientia Agricultura Sinica, 2022, 55(2): 248-264.
[10] LU Qi,JIA XuChao,DENG Mei,ZHANG RuiFen,DONG LiHong,HUANG Fei,CHI JianWei,LIU Lei,ZHANG MingWei. Effects of Different Drying Methods on Bioactive Components of Shatianyou (Citrus grandis L. Osbeck) Pomace Powder [J]. Scientia Agricultura Sinica, 2022, 55(14): 2825-2836.
[11] WANG Juan, MA XiaoMei, ZHOU XiaoFeng, WANG Xin, TIAN Qin, LI ChengQi, DONG ChengGuang. Genome-Wide Association Study of Yield Component Traits in Upland Cotton (Gossypium hirsutum L.) [J]. Scientia Agricultura Sinica, 2022, 55(12): 2265-2277.
[12] HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[13] WANG Yong,LI SiYan,HE SiRui,ZHANG Di,LIAN Shuai,WANG JianFa,WU Rui. Prediction and Bioinformatics Analysis of BLV-miRNA Transboundary Regulation of Human Target Genes [J]. Scientia Agricultura Sinica, 2021, 54(3): 662-674.
[14] FAN WeiGuo,PAN XueJun,HE ChunLi,CHEN Hong,ZHOU YuJia. Accumulation of Sugar and Flavonoids as Well as Their Association with Changes of Light Intensity During Fruit Development of Rosa roxburghii [J]. Scientia Agricultura Sinica, 2021, 54(24): 5277-5289.
[15] ZHANG PengFei,SHI LiangYu,LIU JiaXin,LI Yang,WU ChengBin,WANG LiXian,ZHAO FuPing. Advance in Genome-Wide Scan of Runs of Homozygosity in Domestic Animals [J]. Scientia Agricultura Sinica, 2021, 54(24): 5316-5326.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd