Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (1): 190-195 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Expression of goose interferon γ mature protein and detection of recombinant proteins bioactivity

,,,   

  1. 东北农业大学动物医学院
  • Received:2005-09-19 Revised:1900-01-01 Online:2007-01-10 Published:2007-01-10

PDF

1154

Abstract: the IFN genes, which encode goose interferon γ mature protein, were cloned from total RNA was derived from PHA-stimulated-cultured (PBMCs) of goose by reverse transcription polymerrase chain reaction (RT-PCR). The amplified products of PCR were cloned into pMD18-T vector and sequenced. Compared with the published goose IFN-γ genes sequence, the homology of nucleotide sequence were 100.00%. The fragments of IFN-γ were subcloned into plasmid pET30a and transfer plasmid pMelBacA, pBlueBacHis2A, respectively. the purified recombinant baculovirus were obtained. goose interferon γ mature proteins were successfully expressed in the prokaryotic and eukaryotic expression systems, respectively. And prepared polyclonal antibodies against the products of the prokaryotic expression. There have the common antigenic between the products of prokaryotic and eukaryotic expression systems. One of the biological activity of recombinant mature goose IFN-γ is the ability of activating macrophage of goose to generate nitric oxide(NO) was detected in vitro.

Key words: Interferon(IFN), mature protein, prokaryotic expression, eukaryotic expression, biological activity

[1] Xiang XU,Yi XIE,LiYun SONG,LiLi SHEN,Ying LI,Yong WANG,MingHong LIU,DongYang LIU,XiaoYan WANG,CunXiao ZHAO,FengLong WANG,JinGuang YANG. Screening and Large-Scale Preparation of dsRNA for Highly Targeted Degradation of Tobacco Mosaic Virus (TMV) Nucleic Acids [J]. Scientia Agricultura Sinica, 2021, 54(6): 1143-1153.
[2] XiaoHe LIU,GuiSheng QIU,ZhaoGuo TONG,HuaiJiang ZHANG,WenTao YAN,Qiang YUE,LiNa SUN. Ligands Binding Characteristics of Chemosensory Protein CsasCSP16 of Carposina sasakii [J]. Scientia Agricultura Sinica, 2021, 54(5): 945-958.
[3] YAN DuoZi,CAI Ni,WANG Feng,NONG XiangQun,WANG GuangJun,TU XiongBing,ZHANG ZeHua. Expression in vitro of Metarhizium anisopliae Adhesin MAD1 and Its Effect on Inducing Response in Peanut [J]. Scientia Agricultura Sinica, 2021, 54(4): 744-753.
[4] XU HuanHuan,LI Yi,GAO Wei,WANG YongQin,LIU LeCheng. Cloning and Identification of γ-Glutamyl Transpeptidase AcGGT Gene from Onion (Allium cepa) [J]. Scientia Agricultura Sinica, 2021, 54(19): 4169-4178.
[5] QIN JianHui,LI JinQiao,ZHAO Xu,LI KeBin,CAO YaZhong,YIN Jiao. Expression, Purification and Functional Analysis of Odorant Binding Protein 11 (OBP11) in Anomala corpulenta [J]. Scientia Agricultura Sinica, 2021, 54(14): 3017-3028.
[6] XIE KunLun,LIU LiMing,LIU Mei,PENG Bin,WU HuiJie,GU QinSheng. Prokaryotic Expression of dsRNA of Zucchini yellow mosaic virus and Its Control Efficacy on ZYMV [J]. Scientia Agricultura Sinica, 2020, 53(8): 1583-1593.
[7] ZHANG YuFei,CAO ManYuan,WANG LiYing,ZHAO WeiGang,LI XiaoXia,CHANG Tong,XU BaoZeng. Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein [J]. Scientia Agricultura Sinica, 2020, 53(5): 1058-1070.
[8] LI YongHua, CHE LuPing, QIU XuSheng, TAN Lei, SUN YingJie, LIU WeiWei, SONG CuiPing, LIAO Ying, DING Chan, WANG JinQuan, MENG ChunChun. Construction of Chicken TIGAR Gene Eukaryotic Expression Plasmid and Evaluation of Its Anti-Apoptotic Function [J]. Scientia Agricultura Sinica, 2019, 52(6): 1102-1109.
[9] BI KeRan,LI Yin,HAN KaiKai,ZHAO DongMin,LIU QingTao,LIU YuZhuo,HUANG XinMei,YANG Jing. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Oligoadenylate Synthase-Like Protein [J]. Scientia Agricultura Sinica, 2019, 52(23): 4429-4436.
[10] Ling LI,Yao TAN,XiaoRong ZHOU,BaoPing PANG. Molecular Cloning, Prokaryotic Expression and Binding Characterization of Odorant Binding Protein GdauOBP20 in Galeruca daurica [J]. Scientia Agricultura Sinica, 2019, 52(20): 3705-3712.
[11] LI Du, NIU ChangYing, LI FengQi, LUO Chen. Binding Characterization of Odorant Binding Protein OBP56h in Drosophila suzukii with Small Molecular Compounds [J]. Scientia Agricultura Sinica, 2019, 52(15): 2616-2623.
[12] WANG Hui,CHAI ZhiXin,ZHU JiangJiang,ZHONG JinCheng,ZHANG ChengFu,Xin JinWei. Cloning and Identification of Long-Chain Non-Coding RNA Linc24063 and Its Correlation with the Expression Level of miRNAs in Yak [J]. Scientia Agricultura Sinica, 2019, 52(14): 2538-2547.
[13] YI Min,LÜ Qing,LIU KeKe,WANG LiJun,WU YuJiao,ZHOU ZeYang,LONG MengXian. Expression, Purification and Localization Analysis of Polar Tube Protein 2 (NbPTP2) from Nosema bombycis [J]. Scientia Agricultura Sinica, 2019, 52(10): 1830-1838.
[14] ZHANG Kui,LI ChongYang,SU JingJing,TAN Juan,XU Man,CUI HongJuan. Expression, Purification and Immunologic Function of Integrin β2 in the Silkworm (Bombyx mori) [J]. Scientia Agricultura Sinica, 2019, 52(1): 181-190.
[15] ZHAO Jie, REN SuWei, LIU Ning, AI XinYu, MA Ji, LIU XiaoNing. Cloning and Expression of Phosphatidylethanolamine Binding Protein in Helicoverpa armigera [J]. Scientia Agricultura Sinica, 2018, 51(8): 1493-1503.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd