Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (01): 215- .

• RESEARCH NOTES • Previous Articles    

Pathotyping of Newcastle Disease Virus by SYBR Green I Real-Time RT-PCR

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  1. 南京农业大学动物医学院
  • Received:2004-07-26 Revised:2005-04-30 Online:2006-01-10 Published:2006-01-10

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Abstract: 【Method】The virulence of Newcastale disease virus (NDV) strains was determined by the lysis site sequences of the viral F protein. Accordingly, two pairs of primers were designed to match such sequences of virulent NDV strains and avirulent strains, respectively. Viral RNA was amplified using the two primers respectively by one-step SYBR Green I RT-PCR, and the virus was pathotypied with the amplification effects of the two pairs of primers and the Tm values of the amplification products. 【Conclusion】Pathotyping results of 8 unknown strains of this assay were as the same as those of Mean Death Time (MDT) and sequencing, which suggested that this assay could be used to pathotype NDV strains.

Key words: Avian, Newcastle disease virus, SYBR Green I Real-time RT-PCR, Pathotype

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