Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (01): 215- .doi: 10.3864/j.issn.0578-1752.at-2004-2588

• RESEARCH NOTES • Previous Articles    

Pathotyping of Newcastle Disease Virus by SYBR Green I Real-Time RT-PCR

,,,,   

  1. 南京农业大学动物医学院
  • Received:2004-07-26 Revised:2005-04-30 Online:2006-01-10 Published:2006-01-10

PDF

865

Cited

9

Abstract: 【Method】The virulence of Newcastale disease virus (NDV) strains was determined by the lysis site sequences of the viral F protein. Accordingly, two pairs of primers were designed to match such sequences of virulent NDV strains and avirulent strains, respectively. Viral RNA was amplified using the two primers respectively by one-step SYBR Green I RT-PCR, and the virus was pathotypied with the amplification effects of the two pairs of primers and the Tm values of the amplification products. 【Conclusion】Pathotyping results of 8 unknown strains of this assay were as the same as those of Mean Death Time (MDT) and sequencing, which suggested that this assay could be used to pathotype NDV strains.

Key words: Avian, Newcastle disease virus, SYBR Green I Real-time RT-PCR, Pathotype

[1] MIAO JiaHao, CUI PengFei, YAN Cheng, WANG CongCong, WANG Yan, CHEN Yuan, CHEN Peng, SHI JianZhong, DENG GuoHua, CHEN HuaLan. Genetic and Biological Characterization of Two H8N4 Subtype Avian Influenza Viruses Isolated from Duck [J]. Scientia Agricultura Sinica, 2026, 59(7): 1576-1586.
[2] YU QingQing, ZHOU XiangYu, LI WenXin, LIU YanJing, WANG Yan, HE XinWen, HE Chen, DENG GuoHua, SHI JianZhong, TIAN GuoBin, BAO HongMei, ZENG XianYing, CHEN HuaLan. Immune Effect Evaluation of Combined Inactivated Vaccine Against Avian Influenza and Newcastle Disease [J]. Scientia Agricultura Sinica, 2025, 58(1): 182-191.
[3] CHEN Yuan, CUI PengFei, SHI JianZhong, ZHANG YuanCheng, YU QingQing, YAN Cheng, ZHANG YaPing, WANG CongCong, ZHANG Jie, WANG Yan, DENG GuoHua, CHEN HuaLan. Biological Characteristics of H6N1 Subtype Avian Influenza Virus from 2019 to 2022 in China [J]. Scientia Agricultura Sinica, 2024, 57(9): 1820-1832.
[4] ZHANG HuiYong, WU HuCong, ZHU GuoQiang, LI GuoHui, YU Yan, YIN JianMei, XUE Qian, ZHOU ChengHao, JIANG YiXiu, SU YiJun, HUANG HuaYun, HAN Wei. Detox Dynamics and Reproductive Performance of Langya Chickens Infected with ALV-J [J]. Scientia Agricultura Sinica, 2024, 57(23): 4815-4824.
[5] MA Qi, HE XinWen, WANG Yan, LIU YanJing, PAN ShuXin, HOU YuJie, SHI JianZhong, DENG GuoHua, BAO HongMei, LIU JingLi, GUO XingFu, MAO ShengGang, HU JingLei, LU Tong, YANG Fan, TIAN GuoBin, ZENG XianYing, CHEN HuaLan. The Protective Efficacy of Commercial Vaccines Against H9N2 Avian Influenza Virus of Branch h9.4.2.5 Isolated in China [J]. Scientia Agricultura Sinica, 2023, 56(15): 3040-3050.
[6] FENG AiQing,WANG CongYing,ZHANG MeiYing,CHEN Bing,FENG JinQi,CHEN KaiLing,WANG WenJuan,YANG JianYuan,SU Jing,ZENG LieXian,CHEN Shen,ZHU XiaoYuan. Pathotype Analysis of Xanthomonas oryzae pv. oryzae in Main Rice Producing Regions of China and Establishment of Differential Hosts of Near-Isogenic Lines [J]. Scientia Agricultura Sinica, 2022, 55(21): 4175-4195.
[7] WANG YanWen,WANG MengJing,ZHANG Hong,GAO XinXin,GUO Jing,LI XuYong. Evolution of Human H9N2 Avian Influenza Virus in China from 1998 to 2021 [J]. Scientia Agricultura Sinica, 2022, 55(20): 4075-4090.
[8] TANG KeZhi,ZHOU ChangYong. Transcriptome Analysis of Citrus reticulata Blanco, cv. Hongjv Infected with Alternaria alternata Tangerine Pathotype [J]. Scientia Agricultura Sinica, 2020, 53(22): 4584-4600.
[9] LI YongHua, CHE LuPing, QIU XuSheng, TAN Lei, SUN YingJie, LIU WeiWei, SONG CuiPing, LIAO Ying, DING Chan, WANG JinQuan, MENG ChunChun. Construction of Chicken TIGAR Gene Eukaryotic Expression Plasmid and Evaluation of Its Anti-Apoptotic Function [J]. Scientia Agricultura Sinica, 2019, 52(6): 1102-1109.
[10] JIA YunHui,XU ChengZhi,SUI JinYu,WU YunPu,XU BangFeng,CHEN Yan,YANG HuanLiang,QIAO ChuanLing,CHEN HuaLan. Immunogenicity Evaluation of Eukaryotic Expressing Plasmids Encoding HA Protein of Eurasian Avian-Like H1N1 Swine Influenza Virus [J]. Scientia Agricultura Sinica, 2019, 52(5): 930-938.
[11] LI Li, DU XIN, ZHANG LiNa, YANG Liu, GAO XiaoQing, TANG DongXue, ZHAO HaiYuan, JIANG XiaoMei, ZHANG TianShu, LI JinXiang. Optimization of Cultivation Conditions for Reassortant Avian Influenza Virus H7N9 H7-Re1 Strain [J]. Scientia Agricultura Sinica, 2018, 51(17): 3415-3426.
[12] CHEN Hong, YANG Liu, SONG HaiYan, SHI Ying, MENG LingWei, FU ChunJie, Zhang Dan, ZHAO HaiYuan, LI JinXiang, JIANG XiaoMei, ZHANG TianShu. Domestication of Suspended MDCK Cells and Cultivation of H5 Subtype Avian Influenza Virus [J]. Scientia Agricultura Sinica, 2018, 51(17): 3405-3414.
[13] YANG JinSong, WU Tao, LI JinXiang. Quality Analysis and Development Direction of Recombinant Avian Influenza Inactivated Vaccine (H5 subtype) [J]. Scientia Agricultura Sinica, 2018, 51(17): 3397-3404.
[14] GU Xue-ying, SHI Wen-xiao, WANG Hong-kai, GUO Qing-yuan. Development of the Agrobacterium tumefaciens Mediated Transformation System of Alternaria alternata Apple Pathotype [J]. Scientia Agricultura Sinica, 2016, 49(10): 1885-1891.
[15] WANG Sheng, XIE Zhi-xun, HUANG Li, XIE Li-ji, DENG Xian-wen, XIE Zhi-qin, LIU Jia-bo, LUO Si-si, ZENG Ting-ting. Expression and Identification of the σ3 Gene of Avian Reovirus in Transgenic Tobacco [J]. Scientia Agricultura Sinica, 2015, 48(9): 1836-1844.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd