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Table of Content

    10 January 2006, Volume 39 Issue 01
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES
    Studies on the Growth Habits and Characteristics of Two Polyploid Indica-japonica Hybrids with Powerful Heterosis
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    Scientia Agricultura Sinica. 2006, 39(01):  1-9 . 
    Abstract ( 1529 )   PDF (305KB) ( 1524 )   Save
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    【Objective】Based on a series of polyploid indica-japonica hybrid plant lines obtained from a new breeding strategy of using double heterosis of wide cross and polyploidization to select super rice, two polyploid indica-japonica hybrids, PSR073 and PSR120 were studied in their growth period to show the powerful heterosis in a larger scale and study the characters of polyploid indica-japonica hybrids more completely. 【Method】The leaf age, tiller growth, flowering habit and morphology characteristics of them were observed to analyze their growth habits and characteristics. 【Result】The results showed that the agronomic traits of PSR073 and PSR120, such as plant height, length of panicles, grain length, grain width and 1000-grain weight, all took on obvious predominance of polyploid. And the seed-setting percentage was more than 83%. No difference was observed between the two tetraploids and common diploids in leaf age, tiller growth and flowering habit. 【Conclusion】It is concluded that the characteristics of the two powerful heterosis polyploid hybrids are different from those of the polyploid rice reported before. Wide cross and polyploidization have no negative effects on the growth habits and characteristics of them, on the contrary they have powerful heterosis. This has provided theoretic and practical evidences for their application to agricultural production.
    Molecular Mapping of Two Novel Stripe Rust Resistance Genes YrTp1 and YrTp2 in A-3 Drived from Triticum aestivum × Thinopyrum ponticum
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    Scientia Agricultura Sinica. 2006, 39(01):  10-17 . 
    Abstract ( 1304 )   PDF (835KB) ( 1117 )   Save
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    【Objective】Loss of varietal resistance to stripe rust (Puccinia striiformis Westend f. sp. tritici) is an important factor causing massive periodical epidemic of rust in China′s wheat producing areas. Creation and development of new races of rust pathogen have led widely cultivated varieties into serious crisis of resistance loss. So searching new resistance genes is a long-term strategic task. 【Method】 and 【Result】A-3 line with high resistance (immunity) to current epidemic yellow rust races (CY29, 31, 32) was screened out in offspring of Triticum aestivum × Thinopyrum ponticum. Segregation in F2 and BC1 populations indicated that the resistance was controlled by two independent genes, a dominant gene and one recessive gene, respectively. SSR markers were employed to map the two resistance genes in the F2 and BC1 population. A marker WMC477-167bp locating on 2BS was linked to the dominant gene with genetic distance of 0.4 cM. Another marker WMC364-208bp locating on 7BS was linked to the recessive resistance gene with genetic distance of 5.8 cM. The two genes identified in this paper might be two novel stripe rust resistance genes, which were temporarily designated as YrTp1 and YrTp2, respectively. 【Conclusion】Good resistance to CY31 and CY32, availability of the tightly linking SSR markers can assist rapid transfer of the resistance genes into new varieties to control the epidemic of the two races in wheat production.
    Analysis on Gene Effect of Four Characters of Immature Embryo Culture in Maize
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    Scientia Agricultura Sinica. 2006, 39(01):  18-22 . 
    Abstract ( 1261 )   PDF (191KB) ( 1119 )   Save
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    【Objective】The research aim to pick out the hereditary variation regularity for the main characters of the maize immature embryo culture. 【Method】Two kinds of inbred-line, R18-599 (hong) with very excellent embryo culturing capacity and R15 with very bad embryo culturing capacity, were used as P1 and P2 for obtaining 6 generations. By culturing immature embryos of the six generations, four culturing characters, embryonic callus induction efficiency, non-embryonic callus induction efficiency, embryonic callus clone ability and number of regenerating plant, were analyzed with the general mean analysis and generation joint analysis.【Result】Results showed that the embryonic callus induction efficiency accorded with two major additive-domiance-epistatic genes plus polygene mixed additive-domiance-epistatic inheritance model. The non-embryonic callus induction efficiency accorded with two major additive-domiance-epistatic genes. The number of regenerating plant accorded with one major gene plus polygene mixed additive-dominance inheritance model. The embryo callus clone ability accorded with two major genes plus polygene mixed inheritance model while the epistatic gene effect on this character identified differently by the two methods. 【Conclusion】By comparison of the results of the two methods, generation joint analysis may not only raise experimental precision, but also provide more genetic information.
    Analysis of Differential Gene Expression Pattern in Brassica napus Hybrid Huayouza6 and Its Parents Using Arabidopsis cDNA Microarray
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    Scientia Agricultura Sinica. 2006, 39(01):  23-28 . 
    Abstract ( 1314 )   PDF (310KB) ( 1440 )   Save
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    【Objective】 The purpose of this study is to probe the mechanism of heterosis of Brassica napus in gene expression level, and to accumulate the experimental data for final demonstrating the genetic basis of heterosis.【Method】Two cDNA-based Arabidopisis microarrays were used to analyze gene differential expression in bud of an elite B.napus hybrid Huayouza6 and its parents.【Result】There were 83 over-expression transcripts and 331 under-expression transcripts between Huayouza6 and its female parent 8086A and 94 over-expression transcripts and 423 under-expression transcripts were demonstrated between Huayouza6 and its male parent 7-5. Further analysis showed that there were significant number of genes responsible for photosynthesis, which its implication for heterosis was discussed. Northern analysis of phosphoribulokinase coincided with its expression pattern derived from hybridization of Arabidopsis cDNA microarray and B. napus mRNA. 【Conclusion】This system of heterologous hybridization analysis should be applicable to other close relatives of Arabidopsis thaliana, and basic metabolism genes exert significant influence on heterosis of Brassica napus.
    Advances in Breeding for Super High-Yielding Soybean Cultivars
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    Scientia Agricultura Sinica. 2006, 39(01):  29-37 . 
    Abstract ( 1085 )   PDF (270KB) ( 1432 )   Save
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    This article summarized the recent high yield records in the world, defined the concept of super high-yielding cultivars of soybean, and then proposed a set of yield standards for super high-yielding cultivars in four different soybean-producing regions in China. The advances in the improvement of yield and its related traits, breeding for ideo-type, and effort in utilizing heterosis of soybeans were reviewed. Based on that, the strategies in developing super high-yielding soybean cultivars were discussed.
    TILLAGE & CULTIVATION·PHYSIOLOGY & ECOLOGY
    Relationship Between Root Chemical Signals and Grain Quality of Rice
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    Scientia Agricultura Sinica. 2006, 39(01):  38-47 . 
    Abstract ( 1421 )   PDF (378KB) ( 1319 )   Save
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    【Objective】In order to elucidate the relationship between root chemical signals and the quality of rice.【Method】Using various rice genotypes as materials, zeatin (Z) + zeatin riboside (ZR), abscisic acid (ABA), 1-aminocylopropane -1-carboxylic acid (ACC) and organic acids in roots during grain filing period and the appearance quality, cooking/eating quality were investigated. The correlations among them were analyzed.【Result】The results showed that Z+ZR concentrations in the roots at mid and late grain filling stages were significantly and positively correlated with the gel consistency and alkali spreading value (r=0.72* to 0.90**), whereas negatively correlated with the amylose content (r=-0.68* to -0.78**). ABA concentrations in roots at mid grain filling stage were significantly and negatively correlated with gel consistency and alkali spreading value (r=-0.90** to -0.91**), and positively correlated with the amylose content (r=0.87**). ACC concentrations in root exudates at mid grain filling stage were very significantly correlated with the percentage of chalky grains and chalkiness (r = 0.97** to 0.98**), and those at late grain filling stage were significantly correlated with chalkiness and chalky size (r = 0.69* to 0.96**). The more the malic acid and succinic acid exuded from roots for a cultivar, the greater the breakdown values and smaller the setback values in the starch profile, and the results were reversed for a cultivar with more tartaric acid and citric acid exuded from roots during the grain filling period. The cultivar with more lactic acid in exudates had smaller gel consistency and alkali spreading values, but had greater amylose content. When roots were treated with exogenous ZR, ABA and ACC during grain filling period, the effects of the chemicals on the rice quality were consistent with the relationships of the endogenous hormones (Z+ZR, ABA and ACC) with the quality indexes.【Conclusion】The results suggest that root chemical signals play important roles in the formation of rice quality, and rice quality could be improved through regulating the signals.
    Analysis of the Factors Influencing Surface Temperature in Summer Maize Field
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    Scientia Agricultura Sinica. 2006, 39(01):  48-56 . 
    Abstract ( 998 )   PDF (580KB) ( 1074 )   Save
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    【Objective】The variation regularity and characteristics of surface temperature in field are important for monitoring soil and crop water status.【Method】Energy flux and surface temperature at field scale were differently measured using the eddy covariance technique and remote sensing in summer maize field in the North China Plain, the effect of energy balance components and air temperature on the diurnal and seasonal variation of surface temperature was analyzed during the whole growth and development period of maize.【Result】The relationship between surface temperature and air temperature in diurnal pattern was remarkable different for the difference of leaf area index (LAI) under no-water stress. It was a good linear correlation between them at 0.01 level under small LAI, but complex during full cover period. Net radiation and latent heat flux were the main energy factors influencing surface temperature, the variation of correlation degree between them showed a parabola curve with LAI increasing and decreasing, and there was a highest correlation between surface temperature and energy balance components during full cover period under clear day(α=0.01). There was a close correlation between average daily surface temperature and average daily air temperature, transient surface temperature and air temperature in the seasonal pattern. The value of the surface-air temperature difference was about -5.3℃ under clear day when the soil water content was above about 65% of field capacity in depth of 0 cm-100 cm.【Conclusion】In conclusion, surface temperature is determined by many factors, such as crop growth potential, radiation and soil water conditions and so on.
    Quantitative Evaluation of Land Use Efficiency Under Different Cropping Patterns
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    Scientia Agricultura Sinica. 2006, 39(01):  57-60 . 
    Abstract ( 1429 )   PDF (231KB) ( 1331 )   Save
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    【Objective】To counter the problems existed in application of land equivalent ratio in intercropping, the paper has analyzed that the essence of land equivalent ratio is to estimate land use efficiency of various cropping patterns. 【Method】The method of theoretical analysis combining with experimental data was used. 【Result】On the basis of revealing the essence of land equivalent ratio, combined with living examples, the method of estimating land use efficiency of various cropping patterns, such as sequential cropping, intercropping, and crop rotation was studied. It is cleared that land equivalent ratio is an objective index of estimating land use efficiency of various cropping patterns. 【Conclusion】It was found that the land equivalent ratio of sequential cropping, intercropping, and crop rotation is 1.68, 1.38 and 1.21. Compared with single cropping, the land use efficiency under different cropping patterns is increased by 68%, 38% and 21%, respectively.
    PLANT PROTECTION
    Analysis of Synergetic Induction of Hypersensitive Response by Nitric Oxide and Hydrogen Peroxide in Rice Suspension Cultured Cells
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    Scientia Agricultura Sinica. 2006, 39(01):  61-65 . 
    Abstract ( 1384 )   PDF (330KB) ( 1484 )   Save
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    【Objective】The hypersensitive response (HR) in rice suspension cultured cells induced by exogenous addition of the signal molecules, nitric oxide (NO) and hydrogen peroxide (H2O2) was studied. 【Method】The HR of rice cells induced by NO and H2O2 dependently and/or in synergy, was quantitatively determined by the bromphenol blue staining method.【Result】Results showed that HR of rice cells could be induced by NO and H2O2 dependently and/or in synergy and that NO-induced HR could be regulated by H2O2. The balance interaction in concentrations between NO and H2O2 had no influence on the induction of HR.【Conclusion】NO and H2O2 were the signal molecules for the induction of the rice cells HR and the balance model for NO and H2O2 interaction was not proved in the induction of the HR of rice cells.
    Effects of the MEK-Specific Inhibitor U0126 on the Conidial Germination, Appressorium Production and Pathogenicity of Setosphaeria turcica
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    Scientia Agricultura Sinica. 2006, 39(01):  66-73 . 
    Abstract ( 1117 )   PDF (460KB) ( 1059 )   Save
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    【Objective】Systematic studies on the effects of MAPK (Mitogen activated protein kinase) signal transduction pathway on the growth, development and pathogenicity of Setosphaeria turcica will not only help us to understand the molecular mechanism of pathogen-host interaction, but also benefit the effective control of the diseases caused by S. turcica.【Method】U0126, the specific MEK inhibitor, was used to treat S. turcica before observing the conidial germination, appressorium production and pathogenicity of the pathogen.【Result】There was no significant effect of U0126 on the pathogen's colony morphology and mycelium growth. After treatment with U0126, the mycelium and conidia were normal, but the conidial germination, appressorium production and pathogenicity to susceptible corn leaves were significantly inhibited. Under the definite concentration scope, U0126 increased the inhibition degree to conidial germination and appressorium production with the increase of U0126 concentration, but the inhibition degree decreased with elongation of the treatment time.【Conclusion】The conidial germination, appressorium production and pathogenecity to susceptive corn leaves are regulated by the MAPK pathway inhibited by U0126.
    Homologic Analysis of tuf Gene for Elongation Factor Tu of Phytoplasma from Wheat Blue Dwarf
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    Scientia Agricultura Sinica. 2006, 39(01):  74-80 . 
    Abstract ( 1181 )   PDF (453KB) ( 1096 )   Save
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    【Objective】The aim is to analyze the phytoplasma tuf gene of wheat blue dwarf (WBD). Then this study can differentiate and classify phytoplasma of WBD from subgroup.【Method】 Diseased wheat was observed under the electron microscope and primers (fTufu/rTufu) for phytoplasma tuf gene(EF-Tu) were used to amplify DNA samples extracted from infected wheats, then the amplified fragment was transformed into the plasmid, sequenced and analysed. 【Result】Phytoplasmas were observed in the phloem sieve elements of leaf tissues from naturally-infected wheat under the electron microscope. A 850 bp DNA fragement was amplified in DNA samples from infected wheats but not in those extracted from healthy plants. The sequencing result revealed that the tuf gene of phytoplasma from wheat blue dwarf was 842 bp in length, which encoding 280 amino acids. Homology analysis for sequences of tuf gene from 15 kinds of phytoplasmas showed that the tuf gene of WBD was closely related to those of clover phyllody (KVF), homology rate of nucleotide sequences and amino acids was 99.9% and 100%, respectively.【Conclusion】Homology analysis for sequences of tuf gene can differentiate and classify phytoplasma of wheat bule dwarf from subgroup, thus providing a theoretical basis for research of the phytoplasma origin and molecular nosogenesis of wheat bule dwarf.
    Study on Active Oxygen Quantum Yield, Insecticidal Activities and Stability of Diphenylthiophene
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    Scientia Agricultura Sinica. 2006, 39(01):  81-87 . 
    Abstract ( 933 )   PDF (416KB) ( 1130 )   Save
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    【Objective】Active oxygen quantum yield, insecticidal activities and stability of diphenylthiophene were compared to α-terthienyl in a study for the first time. 【Result】The results showed that absorbency variety max of diphenylthiophene was 0.438 after ultraviolet irradiation for 280 min while α-terthienyl absorbency variety max was 0.480 after ultraviolet irradiation for 200 min. LC50 value of diphenylthiophene and α-terthienyl against 3rd instar larvae of Aedes albopictus was 9.18×10-3 μg·ml-1 and 9.69×10-4 μg·ml-1 after 24 h of treatment. LC50 value of the two pesticides against 3rd instar larvae of Plutella xylostella was 267.87 μg·ml-1 and 222.22 μg·ml-1 after 24 h of treatment. The half-life of diphenylthiophene and α-terthienyl in methanol was 113.62 h and 10.49h. Quantum yield of diphenylthiophene and α-terthienyl was almost identical and they all had high toxicity to Aedes albopictus and Plutella xylostella, but diphenylthiophene was stable than α-terthienyl. 【Conclusion】It is concluded that diphenylthiophene has avoided the deficiency of photoactivated insecticides which degraded quickly in the environment and could not apply in the farm. On the one hand diphenylthiophene could kill the insects, on the other hand diphenylthiophene could accelerate the degradation ratio of triazophos. Diphenylthiophene is worth exploiting and applying in the field.
    Genetic Toxicology Study of sf9 Cell and AcMNPV Treated by HgCl2
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    Scientia Agricultura Sinica. 2006, 39(01):  88-94 . 
    Abstract ( 888 )   PDF (391KB) ( 1015 )   Save
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    【Objective】In an experiment, sf9 cell of Autographa californica was used as an acceptor to inspect the effect of HgCl2 on its growth and development. 【Method】Evaluated the vigor of cells by repulsion of Trypan Blue dyeing method, detected the microkernel in the cells by HE dyeing method, amplified the DNA of HgCl2 treated AcMNPV by PCR, and analyzed the molecular mutation of the products after sequencing. 【Result】The results showed that the surface of sf9 cell became rough, and the division became slowly when treated with 4 μg·ml-1 HgCl2. When the dosage increased to 7 ?g·ml-1, the cell membrane broken. The cell integrity was damaged when the concentration increased to 9 ?g·ml-1. The microkernel in the cells was detected by using HE dyeing method. When treated with 9 ?g·ml-1 HgCl2, the microkernel was as high as 6.8%. Some cells found tri-nucleus and multi-nucleus, reflecting that the integrity of cell was damaged. After shortly treated with HgCl2 AcMNPV was inoculated to sf9 cell. The formation of polyhedra in treated sf9 cells was less than that in CK, and the abnormal polyhedra increased. DNA was extracted from AcMNPV treated with HgCl2, and inspected by PCR. The sequencing of amplification product showed that absent and change (such as G→C, T→C) were found in two bases of DNA. 【Conclusion】Certain dosage of HgCl2 can cause the damage and mutation of sf9 cell of Autographa californica and AcMNPV.
    SOIL & FERTILIZER·WATER-SAVING IRRIGATION
    Effects of Magnesium Compound Fertilizer on Daylily (Hemerocallis citrina Baroni) Growth and Soil Nutrients
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    Scientia Agricultura Sinica. 2006, 39(01):  95-101 . 
    Abstract ( 917 )   PDF (288KB) ( 1180 )   Save
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    【Objective】This paper was to explore the effects of magnesium in dry lands of red soil on the growth and yield of crops, especially for that with low fertility, and to give guidance for application and development of magnesium compound with fertilizer. 【Method】The effects of two kinds of magnesium compound fertilizer on daylily (Hemerocallis citrina Baroni) growth, yield and soil nutrients in red soil were studied, and the change of soil nutrients and daylily growth was observed systemically in field experiments. 【Result】The results showed the favorable effects of magnesium compound fertilizers on improving daylily growth, and increasing its yields and strengthening disease resistance. The effects of magnesium compound fertilizer II (higher Mg content) were better than others, which increased daylily yield by 57.4% and 32.8% than that of in control and NPK treatments, respectively, increased daylily yield by 14.5% as compared with the magnesium compound fertilizer I (lower Mg content). In addition, alkalizable N, available P, exchangeable K and Mg in soil treated with magnesium compound fertilizer II were increased by 94.9%, 46.5%, 31.1% and 35.3% as compared with the control. 【Conclusion】It is concluded that application of magnesium compound fertilizers on red earth is a good method of keeping higher yield and higher quality of crops and soil fertility.
    Effect of Long-Term Fertilization on Utilization and Accumulation of Phosphate Nutrient in Fluvo-Aquic Soil
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    Scientia Agricultura Sinica. 2006, 39(01):  102-108 . 
    Abstract ( 1439 )   PDF (276KB) ( 1000 )   Save
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    【Objective】Nine fertilization treatments including fertilizers, manure, maize straw on nutrient balance and nutrient efficiency in wheat-maize rotation system were studied. 【Method】 Long-term fertilization experiments in alluvial soil were started in 1990 in experimental farm in Henan Academy of Agricultural Sciences in Zhengzhou of China′s Hennan Province.【Result】The results showed that the mean inputs of P was 2.4-3.1 kg·ha-1, and 16.7-21.6 kg·ha-1 of P were absorbed by wheat and maize, thus resulting in the shortage of 14.3-18.5 kg·ha-1 of P nutrient, annually in treatments without P fertilizer application after 14 years of wheat-maize rotation. Except 78 kg·ha-1 and 107 kg·ha-1 (1.5MNPK) of P nutrient from P fertilizer, the P nutrient from manure, maize straw, rain water and irrigations, seeds were about 2.7-134 kg·ha-1 entered into soil annually, in which 9.2%-38.3% were recovered by wheat and maize. The accumulated recovery rate of P in wheat was 4.4%-50.8%, varied greatly with different fertilization, the order was: NPK>NP>SNPK>MNPK>1.5MNPK>PK; The accumulated recovery rate of P in maize was 13.4%-36.1%, and the order was: 1.5MNPK> SNPK>NPK> NP> MNPK>PK. 【Conclusion】 It is concluded that the more the amount of P nutrient input, the lower the P use efficiency. Among the P entered in soil, there were appropriately 52-210 kg·ha-1 of P accumulated in 0-40 cm soil annually, 38%-60% changed into soil total P (H2SO4-HClO4 method), 6.7%-13.6% transformed into available-P with more in manure and less in inorganic fertilization treatments; At least 40%-62% of phosphate were extravagant.
    Characteristics of Soil Fertility of Buried Ancient Paddy at Chuodun Site in Yangtze River Delta
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    Scientia Agricultura Sinica. 2006, 39(01):  109-117 . 
    Abstract ( 1319 )   PDF (263KB) ( 794 )   Save
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    【Objective】 A study was carried out to explicate the basic characteristics of ancient paddy soils at Chuodun site, in order to achieve better comprehension of the mechanisms of the sustainability of paddy soil. 【Method】 Soil age and the distribution of buried ancient paddy at Chuodun site in Yangtze River Delta were determined using 14C dating and other archeological methods. Soil pH, organic C, and nutrients were also investigated in total 20 ancient paddy soils based on the methods compiled by Society of Soil Science of China. 【Result】The oldest paddies at Chuodun site can be dated back to Neolithic age, around 6 000 aBP. These ancient fields were buried at approximately one-meter depth below top-layer, whose areas were ranged from 0.32 m2 to 12.9 m2 with an average of 5.2 m2. Differences of soil nutrients were determined depended on fields and rice planting intensity. The contents of soil total C, N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn were 9.7, 0.8, 1.58, 18.2, 7.6, 6.3, 0.1, 22.7, 0.51 g·kg-1 and 40.9, 80.8 mg·kg-1, respectively. 【Conclusion】Soil organic C, total N, and C/N ratio were obviously greater in ancient paddy (rice plant phytolith > 5 000 /g) than in less-cultivated paddies (rice plant phytolith < 5 000 /g. No significant differences were found in other nutrients, soil pH and available nutrients. Soil total N, S, Cu contents were obviously less in ancient paddy soils than those in recent paddy soils, while the contents of soil total C, P, Fe, Mn were the contrary. Available nutrient contents were generally greater in recent paddy soils than those in ancient paddy.
    Soil Phosphorus Release to the Water Bodies in the Upland Fields of Yellow Soil Areas and the Influencing Factors
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    Scientia Agricultura Sinica. 2006, 39(01):  118-124 . 
    Abstract ( 1316 )   PDF (259KB) ( 857 )   Save
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    【Objective】Soil phosphorus release to the water bodies in the upland fields of yellow soil areas and influencing factors was studied in Guizhou province. 【Method】Adopting by the no boundary runoff little section, pot and simulating experiment.【Result】The results showed that the content of dissolved active P of surface runoff from various upland fields of yellow-soil were significantly different, which the concentrations of dissolved active P of runoff correlated with the contents of available-P, amorphous oxides of Al, and organic matter in the soils. The amount of soil phosphorus release to the water bodies affected by the level of applying P fertilizer and the process of maize growth. When the fertilizer application rate was from 150 to 900 kg·ha-1 (P2O5) in the soil with high P level, the average contents of dissolved active P in the penetrated-water of the soil increased from 0.020 mg·L-1 to 0.137 mg·L-1. The amount of soil phosphorus release to the water bodies also affected by environmental factors. The amount of soil phosphorus release significantly increased on the conditions that the temperature was 30-35℃, water/soil ratio was 15﹕1 to 25﹕1, submerged-time in water was 12-18 h and pH value of acid rain was 3.82-3.73. 【Conclusion】The content of dissolved active P of surface runoff from various upland fields of yellow-soil was significantly different, which significantly positive and negative correlated with available-P, and amorphous oxides of Al and organic matter in the soils, respectively.
    Community Composition and Distribution of Soil Macro-Arthropods Under Agricultural Environment in the Black Soil Region of Jilin Province
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    Scientia Agricultura Sinica. 2006, 39(01):  125-131 . 
    Abstract ( 1119 )   PDF (229KB) ( 1011 )   Save
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    【Objective】In order to learn the effects of land-use on macro-arthropods community. 【Method】The soil macro-arthropods in the black soil region in Jilin Province were investigated with the emphasis laid on the species richness and abundance in relation to the types of land-use i.e. farmland, farm garden and Three-North Forest Shelterbelt. The soil macro-arthropods were hand-sorted in the field. 【Result】A total of 2 357 soil macro-arthropods individuals was captured and fell into 5 classes, 9 orders and 70 families.【Conclusion】The results suggest that the types of land use affect the species richness and abundance, and human activity has a significant impact on the soil macro-arthropods community. The agricultural activities change their vertical distributions in the soil profile, and improve the richness and abundance of macro-arthropods in the lower soil stratum especially in July. The results also show that different soil macro-arthropods are sensitive to changes of land use.
    HORTICULTURE
    Improved Conditions of in vitro Culture of Unpollinated Ovules and Production of Embryonary Sac Plants in Summer Squash (Cucurbita pepo L.)
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    Scientia Agricultura Sinica. 2006, 39(01):  132-138 . 
    Abstract ( 1056 )   PDF (297KB) ( 1337 )   Save
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    【Objective】The aim of this research was to establish a high frequency plants regemeration system for summer squash (Cucurbita pepo L.) via in vitro gynogensis, in order to provide a quick way to abtain self-bred lines for summer squash F1 breeding.【Method】Unpollinated ovules from summer squash ovaries were cultured on N6 medium and supplemented with 2, 4 - D, NAA, BA. After the embryoids were obtained, they were transplanted into N6 medium without growth regulators, and the regenerated plants would be obtained.【Result】120 embryonary sac plants were regenerated in this research. Among them, 41 plants were died of reduced growth, 8 plants died of studying the regenerated method, 71 plants survived after transplanting into soil. In these survived plants, 42 have normal fertility and 10 of them had obtained self-bred fruits and seeds; 29 have abnormal fertility . The statistical results showed that ovules development stage, medium, material genetype and material planting season had significant effects on the embryoids inductivities.【Conclusion】The embryoids came from the cells of embryonary sac, the regenerated plants are embryonary sac plants. 3 mediums had been selected for high inducticities. Ovules from one day before anthesis and the day of anthesis had higher inducticities and the materials planted in autumn was the best for induction of embryoids.
    Analysis of Genetic Diversity of Processing Apple Varieties
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    Scientia Agricultura Sinica. 2006, 39(01):  139-144 . 
    Abstract ( 1112 )   PDF (297KB) ( 1148 )   Save
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    【Objective】 In order to provide information of processing apple varieties (Malus domestica Borkh.) and offer theoretical reference for breeding of processing apple. 【Method】 Genetic diversity of 18 processing and 2 fresh apple varieties were evaluated with 12 simple sequence repeats (SSR) primer pairs previously identified in apple, using cluster analysis and principal coordinate analysis (PCO). 【Result】All the varieties were divided into 4 groups by cluster analysis. High-acid variety Avrolles formed a single group with GS less than 0.42 with other varieties. The second group contained Longfeng and Dolgo from Northeast of China, inheriting Chinese crab apple genes. The 5 cider varieties with high tannin content were clustered into the third group, i.e., Dabinette, Frequin rouge, Kermerrien, Marie Menard, Douce.Coetligne. The fourth group was mainly composed of 12 juice and table apple varieties. The genetic similarity (GS) of the cluster analysis varied from 0.14 to 0.83. Principal coordinate analysis also divided all the varieties into four groups. Both analyses indicated there were genetically abundant diversities among apple processing varieties. 【Conclusion】 There were much difference between cider and juice varieties, cider and table varieties as well as Chinese crab apple and western European crab apple, while juice and table apple varieties had similar genetic background.
    Isolation and Identification of Virus dsRNA from Strawberry Plants
    He Li,,,,,
    Scientia Agricultura Sinica. 2006, 39(01):  145-152 . 
    Abstract ( 1120 )   PDF (888KB) ( 1083 )   Save
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    【Objective】The analysis of virus genome is based on nucleic acid isolation. The aim of this study was to develop the method of isolation and identification of virus dsRNA and elucidate the nucleotide sequences of strawberry virus. 【Method】Using the modified method, the virus dsRNA was extracted from strawberry virus indicator plants and cultivated strawberry plants, and detected by employing agarose gel electrophoresis of EtBr staining and reverse transcription-polymerase chain reaction (RT-PCR). 【Result】The quantity of virus dsRNA was different among strawberry cultivars. The quantity of dsRNA in microplants was higher than in the young leaves of field plants. And for the field plants, there was more dsRNA in the young leaves. The dsRNA was resistant to DNaseⅠ, but it became evidently resistant to RNase A only in the presence of 0.5 mol·L-1 NaCl. The dsRNA in agarose gel could be availably recycled with agarose gel DNA purification kit. The segments of both strawberry mottle virus (SMoV) and strawberry mild yellow edge virus (SMYEV) were amplified by RT-PCR from the virus dsRNA recycled from gel or treated with DNase Ⅰ/RNase A. 【Conclusion】The system of isolation and identification of dsRNA in strawberry plants was developed, which is the base for the genome analysis of strawberry virus isolations in China.
    Separation, Purification and Identification of Antioxidant Compositions in Black Rice
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    Scientia Agricultura Sinica. 2006, 39(01):  153-160 . 
    Abstract ( 1677 )   PDF (425KB) ( 1605 )   Save
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    【Objective】To separate, purify and identify the antioxidant compositions of black rice.【Method】Using total antioxidative capacity (TAC) as an activity monitoring parameter,different fractions of black rice antioxidative extracts were obtained with different polarities of solvents such as petroleum ether, chloroform, ethyl acetate and normal butyl alcohol. The main antioxidative components from the strongest antioxidative fractions were separated by using Sephadex LH-20 resin and the structures were analyzed by ultraviolet-vis, infra-red, ESI-MS, 1H-NMR and 13C-NMR spectrums.【Result】The water fraction and normal-butyl alcohol fraction of antioxidative extracts of black rice of had the strongest antioxidative capacities and their TACs reached 383 ku·g-1 and 392 ku·g-1, respectively. Four main antioxidative components were separated from the water fraction and their TACs reached 976 ku·g-1, 878 ku·g-1, 1 134 ku·g-1 and 1 087 ku·g-1, respectively. The spectroscopy analysis indicated that the 4 active components of the antioxidative extract of black rice were 4 anthocyanin compounds of malvidin, pelargonidin-3, 5-diglucoside, cyaniding-3-glucoside and cyaniding-3, 5-diglucoside. 【Conclusion】 It is concluded that the anthocyanin compounds are the most important substance basis for antioxidation.
    ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT
    Development and Application of a Real-Time PCR Approach for Quantification of Methanobacterium formicium in Rumen
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    Scientia Agricultura Sinica. 2006, 39(01):  161-169 . 
    Abstract ( 1058 )   PDF (320KB) ( 1185 )   Save
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    【Objective】To solve the short comings of the conventional quantitative method in determination of runmen microorganisms. 【Method】A quantitative method for determination of Methanobacterium formicicum in rumen was developed by real-time PCR using 16S rDNA in this experiment. 【Results】The detection limit of this mehod was 30 copies (15 M. formicicum cell ) in 25 l reaction system with the primers, probe and reaction condition developed in this study, which is faster and more sensitive than the traditional mode for quantification of microorganisms. The positive correlation between the population of M. formicicum by real-time PCR and the population of M. formicicum by traditional mode was very high (r=0.957, P<0.01), which showed that the quantitative method by real-time PCR could accurately reflect the variation of rumen microorganism's population. The numbers of M. formicicum in rumina of steers and Holstein cows, were determined respectively. The result showed that the concentration of M. formicicum in rumen fluid of steers was 5.6 times higher than that of Holstein cows.【Conclusion】The quantitative method for determination of M. formicicum in rumen by real- time PCR could accurately reflect the variation of rumen microorganism's population.
    Study on FSHR and LHR mRNA Levels of Different BMPRIB Genotypes from Small Tail Han Sheep During the Oestrum
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    Scientia Agricultura Sinica. 2006, 39(01):  170-175 . 
    Abstract ( 881 )   PDF (447KB) ( 1034 )   Save
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    【Objective】The relation between different BMPRIB genotypes in Small Tail Han sheep and FSH receptor and LH receptor mRNA levels during the oestrum was studied 【Method】Semi-quantitative PCR. 【Result】The results showed that the FSH receptor mRNA levels from the right ovary of BB (1.14±0.11) ewes were higher than those of AA (0.44±0.11) and AB (0.36±0.08) ewes (P<0.01). LH receptor mRNA levels from the right ovary of BB (0.42±0.02) ewes were significantly higher than those of AA (0.23±0.02) and AB (0.25±0.04) ewes (P<0.01), but was no significant difference in the left ovary among the genotypes during the oestrum was observed. 【Conclusion】It is concluded that the higher mRNA levels of FSH and LH receptor may be one of the reasons of fecund Small Tail Han sheep.
    Protein Expression and Function of the vpr Gene of the vpr Isogenic Knockout E. coli Mutant
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    Scientia Agricultura Sinica. 2006, 39(01):  176-180 . 
    Abstract ( 1669 )   PDF (414KB) ( 1097 )   Save
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    【Objective】In order to confirm the function of vpr gene. 【Method】 The complement of the mutant strain MC1061△vpr was constructed by translating intact vpr into the vpr isogenic knockout E. coli mutant and identified using PCR. The lytic susceptibility of VT2 bacteriophage C43b to parent strain MC1061, mutant strain MC1061△vpr, complemented strain were detected. The cell walls of above three strains were extracted, respectively. The test of phage-adsorption to cell wall, Vpr protein expression and blotting were also carried out. 【Result】 One complemented strain, named MC1061-C, was obtained. The complemented strain returned the susceptibility to the lytic infection of VT2 phage with many small plaques, while the mutant was still resistant the phage. Phage-adsorption test within 30 min showed that 94.8% VT phage could adsorb on the cell walls of complemented MC1061-C, while 85.4% VT phage could not adsorb on the cell walls of mutant MC1061△vpr. The specific peptide of 90 000 from parent and complemented strain were displayed by the Western blot using the rabbit anti-Vpr, the His-Vpr was purified by affinity chromatography. The mutant did not display this protein. 【Conclusion】All these revealed that the Vpr supported infection of VT2 phage and most likely be the receptor of VT2 phage.
    Development of an Indirect Enzyme-Linked Immunosorbent Assay for Contagious Bovine Pleuropneumonia Using Recombination Lipoprotein LppQ of Mycoplasma mycoides subsp mycoides SC
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    Scientia Agricultura Sinica. 2006, 39(01):  181-186 . 
    Abstract ( 1211 )   PDF (404KB) ( 971 )   Save
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    【Objective】Mycoplasma mycoides subsp mycoides SC (MmmSC) is the etiological agent of contagious bovine pleuropneumonia (CBPP). The N-terminal domain of the mature lipoprotein LppQ is hydrophobic, and it induces a strong, specific, early and persistent immune response in naturally and experimentally infected animals. People have good reason to believe N-terminal fragment of lipoprotein LppQ will become a kind of ideal CBPP diagnosis antigen.【Method】Mycoplasma-specific TGA (Trp) codons are utilized as stop codons in most other organisms. The lppQ N-terminal fragment from MmmSC HVRI X strain was mutated with a one-step overlapping extension PCR, and was expressed in E. coli. An indirect enzyme-linked immunosorbent assay was established by using recombination lipoprotein LppQ 【Result】Sequence analysis confirmed the successful mutation from A to G in codon 198 in the lppQ gene. The fragment containing the mutation site was subcloned into the pET32a expression vector. The recombinant protein with molecular weight of 42kD was purified by Ni-NTA His·Bind purification kit and the purity up to 95%. Western blot indicated that the standard positive serum of CBPP could react with the recombinant protein. The purified protein was diluted to 0.35 μg·ml-1, and coated to microtiter ELISA plates. Indirect ELISA reaction conditions were optimized. The value of P/N was determined to be 4.8 (0.934/0.193), the sensitivity to be 95.8% (46/48), and the specificity to be 98.9% (161/163). A total of 3 817 bovine serum samples were detected by indirect ELISA and CFT. 【Conclusion】The Kappa value is 0.63, which is middle or high agreemen between the two methods.
    Detection of HSP mRNA Transcription in Transport Stressed Pigs by Fluorescence Quantitative RT-PCR
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    Scientia Agricultura Sinica. 2006, 39(01):  187-192 . 
    Abstract ( 1251 )   PDF (353KB) ( 934 )   Save
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    【Objective】To study the transcription changes of the HSP mRNA during the heat stressing. 【Method】According to the specific sequence of HSP90, HSP70 and GAPDH mRNA, the primers were designed and synthesized. The fragments generated by RT-PCR were cloned into the pGEM-T vector. The positive recombinant plasmids were used to transcript RNA in vitro and the RNA were used as standard quantitative template to make the standard curve and established fluorescence quantitative RT-PCR method. By means of established fluorescence quantitative RT-PCR method, the transcription changes of the HSP70 mRNA and the HSP90 mRNA in livers and hearts of transport stressed pigs were studied after 1 h, 2 h, 4 h, 6 h and 10 h transportation. 【Result】The level of HSP70 mRNA transcription increased continuously from the beginning of transportation. The inductions of HSP70 mRNA transcription in liver and heart of 10-hour-transport stressed pigs were 2.5 and 4.1 times higher than those of the un-transport stressed pigs (P<0.01). However, the transcription levels of HSP90 mRNA in livers and hearts decreased with transport stress. 【Conclusion】Transport stress can affect the transcription of HSPs mRNA, and the effect is different between HSPs families. Furthermore, the concentration of HSP70 mRNA increased continuously from the beginning of transport stress, might be a hallmark to determine stress.
    RESEARCH NOTES
    Identification of the Alien Chromosomes in a Salt-Resistant Wheat-Leymus multicaulis Disomic Addition Line
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    Scientia Agricultura Sinica. 2006, 39(01):  193-198 . 
    Abstract ( 1420 )   PDF (368KB) ( 703 )   Save
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    【Objective】The salt-resistance and the alien addition chromosomes of the addition line Line15 were studied in an experiment. 【Method】The salt-resistance was detected under 0.4% ( NaCl/soil w/w) salt stress in man-made simulated salt pool. The numbers of chromosomes in the root cells and pollen cells was also checked. Then Line15 was detected by fluorescence in situ hybridization (FISH) and simple sequence repeat (SSR).【Result】The results showed that the wheat-Leymus multicaulis offspring Line15 was a salt-resistant line, and it was a disomic alien addition line which karyotype was 2n=44=22II. The results of SSR showed that the two alien addition chromosomes are closely correlated to the long arm of the second homologous group, the centromere region of the third homologous group and the short arm of the seventh homologous group in wheat, respectively. 【Conclusion】In this research, the wheat-Leymus multicaulis disomic alien addition line Line15 was detected on physiological, cellular and molecular level. The research results have provided reliable evidence for the source of the salt-resistance of Line15.
    Trend of Occurrence of Cotton Bollworm and Control Efficacy of Bt Cotton in Cotton Planting Region of Southern Xinjiang
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    Scientia Agricultura Sinica. 2006, 39(01):  199-205 . 
    Abstract ( 1225 )   PDF (441KB) ( 1338 )   Save
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    【Objective】Cotton planting region in southern Xinjiang has become one of the most important regions for China′s cotton production. As a major negative factor in cotton industry, cotton bollworm, Helicoverpa armigera, possesses a biological potential to outbreak in this region. 【Method】 The current research results on trend of occurrence of H. armigera and control efficacy of Bt cotton in southern Xinjiang are reported. 【Result】The monitoring on population density of the pests in wheat field from 1993 to 2004 indicated that as the regulation of cotton-corn planting system in southern Xinjiang, the occurrence and damage of H. armigera increased year by year after 1997, and it has become a main pest in agriculture in comparison with old system containing a small proportion of cotton. The field trials on population dynamics of cotton bollworm on GM cotton varieties expressing Cry1A toxin and Cry1A/CpTI, and their parental varieties during 2002-2004 showed that the second generation bollworm mainly injure cotton, and the control efficacies from both GM cotton varieties were more than 75%. It is suggested that the Bt cotton could effectively control damages caused by cotton bollworm in the moderate outbreak year. 【Conclusion】In view of the severe damage of cotton bollworm, it is necessary to develop a cotton IPM system based on the application of GM cotton varieties in southern Xinjiang.
    Study on the Changes of Ascorbic Acid and Dehydroascorbic Acid Contents in Haw Fruits During Their Processing
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    Scientia Agricultura Sinica. 2006, 39(01):  206-209 . 
    Abstract ( 950 )   PDF (227KB) ( 784 )   Save
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    【Objective】Changes of ascorbic acid (AA) and dehydroascorbic acid (DHA) content in haw fruits were studied during its processing.【Method】The change law of AA and DHA were studied through observing its content during haw fruits pulping, sugar boiling processing and drying. 【Result】Results indicated that adding EDTA-2Na or tea polyphones was helpful for controlling AA loss during pulping, yet (NaPO3)6 was beneficial to controlling DHA in this processing. Vacuum sugar boiling processing could more efficiently save AA content than barometric sugar boiling, but it wasn't effective on DHA content. Drying process greatly influenced on haw AA and DHA content. Vacuum drying and microwave drying process were helpful for saving AA content. 【Conclusion】The fruits of haw contain more DHA, and it changes in the course of processing more obvious and it has prominent influence on TAA content of the haw products.
    Analysis of the M-Like Gene of Streptococcus zooepidemicus Chinese Isolates from Pig
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    Scientia Agricultura Sinica. 2006, 39(01):  210-214 . 
    Abstract ( 874 )   PDF (268KB) ( 925 )   Save
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    【Objective】In order to clarify the features of the M-like gene of Streptococcus equi subsp. zooepidemicus isolates from Chinese pig for directing the selection of vaccine isolate.【Method】The M-like genes were amplified from 9 isolates of Streptococcus equi subsp. zooepidemicus from Chinese pig by PCR and the M-like gene of each isolate was cloned, sequenced and analyzed. 【Result】Nucleotide sequence analysis revealed one open reading frame in M-like protein gene of all isolates, with different length of 1 125 bp in CP-0104 isolate, 1 143 bp in CG-74-63 isolate, and 1 137 bp in the others. The nucleotide identities of M-like genes among 9 isolates except CP-0104 isolate were higher than 95.4%, while the identities between isolate CP-0104 and the remaining 8 strains were 87.1%-88.3%. W60 strain, an equine origin isolate, shared identities of 81.6%-87.0% with these 9 isolates from pig, and 25064 strain, a human origin isolate, shared identities of 81.7%-91.8% with these 9 isolates from pig. All the 9 isolates shared the common membrane anchor region and N-terminal signal peptide. 【Conclusion】The M-like gene of 9 isolates of Streptococcus equi subsp. zooepidemicus from Chinese pig share high identity.
    Pathotyping of Newcastle Disease Virus by SYBR Green I Real-Time RT-PCR
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    Scientia Agricultura Sinica. 2006, 39(01):  215- . 
    Abstract ( 1231 )   PDF (269KB) ( 860 )   Save
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    【Method】The virulence of Newcastale disease virus (NDV) strains was determined by the lysis site sequences of the viral F protein. Accordingly, two pairs of primers were designed to match such sequences of virulent NDV strains and avirulent strains, respectively. Viral RNA was amplified using the two primers respectively by one-step SYBR Green I RT-PCR, and the virus was pathotypied with the amplification effects of the two pairs of primers and the Tm values of the amplification products. 【Conclusion】Pathotyping results of 8 unknown strains of this assay were as the same as those of Mean Death Time (MDT) and sequencing, which suggested that this assay could be used to pathotype NDV strains.