Abstract: 【Objective】To detect bio-safety of Trichoderma, to evaluate the impact on partial ecological factor after the application of Trichoderma.【Method】Inoculate spores of Trichoderma into seeds, soil and water by means of conventional test methods, to detect its survival in water and its effects on rhizosphere microorganisms and growth of melon, as well as toxicity on mice and white rabbits.【Result】The majority of Trichoderma were safe to sweet-melon seeds and could increase significantly germination ratio, the number of fibrous roots and improve the growth of melon seedling. Trichoderma viride T23 did not show significant effects on the number of fungi, bacteria and actinomyces in rhizosphere soil of sweet-melon and water-melon. Spores of Trichoderma could not germinate in river, well water, rainwater and tap water. Conidiaspores and chlamydospores could survive in water for 12-20 days and 20-30 days, respectively. Trichoderma had no harm on the growth of fish. Meanwhile, it had low toxicity on rats in both stomach and skin toxicity experiments, and no allergic reaction to rabbit skin and mucous membrane of eyes.【Conclusion】Trichoderma is in-noxious and has no pollution , as same as other microbial pesticide. It is no risk using Trichoderma to control soil-spreading diseases.

Key words: Trichoderma, Conidiaspores, Chlamydospores, Rhizosphere microorganism, Biology security