Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (04): 813-820 .doi: 10.3864/j.issn.0578-1752.at-2004-2430

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Effects of Compound Polysaccharide Extracted from Traditional Chinese Medical Herbs on the Immunity Function in Chickens

  

  1. 石河子大学动物科技学院
  • Received:2004-05-31 Revised:1900-01-01 Online:2005-04-10 Published:2005-04-10

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Abstract: Four hundred and eighty 7-day-old chickens(Roman) were randomly divided into four groups and with 120 chidkens for each. Eight-day-old chickens were vaccined with the Chinese herbal medicine compound polysaccharide plus cyclophosphamide, the Chinese herbal medicine compound polysaccharide, cyclophosphamide, physiologic saline. All chickens (15 in each group)were bled and smeared before injection, and on the 8th, 17th, 21st, 29th, 37th day post-vaccination, the percentage of lymphocyte and ANAE+ were determined. All chickens (12 in each group) were bled before using medicine and after stopping use medicine, the phagocytosis ability of neutrophil exythrocyte were determined. At 18-, 25- and 32-day-old of age chickens' (12 in each group) phagocytosis function of the abdominal cavity macrophage was determined. At 7th, 14th, 21st day post the frist immunization and at 7th, 14th, 28th day post the second immunization of ND Ⅱ vaccine, all chickens (15 in each group) were bled and serum was sampled, ND-HI antibody titers were determined. At 4-, 6- and 9-week-old of age chickens'(12 in each group)weight of bursa F, thymas and spleen were determined. All chickens (15 in each group) were bled before using medicine, and 4, 8 days after stopping use medicine, immunity function of red blood cell were determined. The results showed that the Chinese herbal medicine compound polysaccharide increased the percentage of lymphocyte transformations and ANAE+, the phagocytosis rate and index of neutrophil erythrocyte, the phagocytosis rate and index of the abdominal cavity macrophage, ND-HI antibody titers after the first and the second immunization, weight of bursa F, thymas and spleen, RBC-C3b rosette rate and RBC-IC rosette rate. Nevertheless, cyclophosphamide decreased all of these values. Significant differences between the group treated with the Chinese herbal medicine compound polysaccharide plus cyclophosphamide and the group treated with cyclophosphamide in these values. The values of group treated with the Chinese herbal medicine compound polysaccharide plus cyclophosphamide were higher than the control group.

Key words: Traditional Chinese medical herbs, Compound polysaccharide, Cyclophosphamid, Chicken, Immunity function

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