Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (04): 821-825 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Fusion of Microvascular Endothelial Cells Induced by Muramidase-Released Protein of Streptococcus suis Type 2

,   

  1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室
  • Received:2004-02-24 Revised:2004-10-26 Online:2005-04-10 Published:2005-04-10

PDF

855

Cited

24

Abstract: Streptococcus suis type 2 (SS2) mainly causes meningitis of human and pigs. Muramidase-released protein (MRP) is one of the key virulence factors of SS2. However，the pathogenic effects of MRP on microvascular endothelial cells (MEC) which makes up of brain blood barrier (BBB) is unknown to date. So the spleen microvascular endothelial cells (SMEC) from neonatal rabbit were isolated, purified, immortalized by SV40-T antigen, and used as cell model in present study. After co-incubation with electrophoretically purified MRP, two principal morphologic changes of SMEC confluent layer were revealed by light microscopy. Firstly, the confluent cell layer turned into a riddle-like structure full of giant hole. Secondly, cells were fused into syncytia which scattered in the hole, containing 10～30 nuclei. The nuclei were then released from the syncytia after an extreme condensation. And finally, the syncytia disappeared. Results of present study suggest that MRP alone is sufficient to destroy the brain blood barrier.

Key words: Streptococcus suis type 2, Muramidase-released protein, Apoptosis, Cell fusion, Microvascular endothelial cells

[1] LIU YuFang,CHEN YuLin,ZHOU ZuYang,CHU MingXing. miR-221-3p Regulates Ovarian Granulosa Cells Apoptosis by Targeting BCL2L11 in Small-Tail Han Sheep [J]. Scientia Agricultura Sinica, 2022, 55(9): 1868-1876.
[2] WANG JiaMin,SHI JiaChen,MA FangFang,CAI Yong,QIAO ZiLin. Effects of Soy Isoflavones on the Proliferation and Apoptosis of Yak Ovarian Granulosa Cells [J]. Scientia Agricultura Sinica, 2022, 55(8): 1667-1675.
[3] LI WenHui,HE YiJing,JIANG Yao,ZHAO HongYu,PENG Lei,LI Jia,RUI Rong,JU ShiQiang. Effects of FB1 on Apoptosis and Autophagy of Porcine Oocytes in vitro Maturation [J]. Scientia Agricultura Sinica, 2022, 55(6): 1241-1252.
[4] MingJie XING,XianHong GU,XiaoHong WANG,Yue HAO. Effects of IL-15 Overexpression on Myoblast Differentiation of Porcine Skeletal Muscle Cells [J]. Scientia Agricultura Sinica, 2022, 55(18): 3652-3663.
[5] YANG ChangPei,WANG NaiXiu,WANG Kai,HUANG ZiQing,LIN HaiLan,ZHANG Li,ZHANG Chen,FENG LuQiu,GAN Ling. Effects and Mechanisms of Exogenous GABA Against Oxidative Stress in Piglets [J]. Scientia Agricultura Sinica, 2022, 55(17): 3437-3449.
[6] FENG YunKui,WANG Jian,MA JinLiang,ZHANG LiuMing,LI YongJun. Effects of miR-31-5p on the Proliferation and Apoptosis of Hair Follicle Stem Cells in Goat [J]. Scientia Agricultura Sinica, 2021, 54(23): 5132-5143.
[7] MA MengNan,WANG HuiMing,WANG MiaoMiao,YAO Wang,ZHANG JinBi,PAN ZengXiang. Identification of circINHBB During Follicular Atresia and Its Effect on Granulosa Cell Apoptosis [J]. Scientia Agricultura Sinica, 2021, 54(18): 3998-4007.
[8] LI RunTing,CHEN LongXin,ZHANG LiMeng,HE HaiYing,WANG Yong,YANG RuoChen,DUAN ChunHui,LIU YueQin,WANG YuQin,ZHANG YingJie. Transient Expression and the Effect on Proliferation and Apoptosis of Granule Cell Stimulating Factor in Ovarian Fibroblasts [J]. Scientia Agricultura Sinica, 2021, 54(11): 2434-2444.
[9] HUANG Feng,WEI QiChao,LI Xia,LIU ChunMei,ZHANG ChunHui. Research Progress on Mechanisms of Apoptosis to Postmortem Tenderization in Muscle [J]. Scientia Agricultura Sinica, 2021, 54(10): 2192-2202.
[10] Xin ZHANG,KongLin HUO,XingXing SONG,DuoNi ZHANG,Wen HU,ChuanHuo HU,Xun LI. Effects of GnIH on Autophagy and Apoptosis of Porcine Ovarian Granulosa Cells via p38MAPK Signaling Pathway [J]. Scientia Agricultura Sinica, 2020, 53(9): 1904-1912.
[11] PAN YangYang,WANG Meng,RUI Xian,WANG LiBin,HE HongHong,WANG JingLei,MA Rui,XU GengQuan,CUI Yan,FAN JiangFeng,YU SiJiu. RNA-Binding Motif Protein 3(RBM3) Expression is Regulated by Insulin-Like Growth Factor (IGF-1) for Protecting Yak (Bos grunniens) Cumulus Cells from Apoptosis During Hypothermia Stress [J]. Scientia Agricultura Sinica, 2020, 53(11): 2285-2296.
[12] CHEN Peng,BAO XiYan,KANG TaoTao,DONG ZhanQi,ZHU Yan,PAN MinHui,LU Cheng. Screening and Identification of Proteins Interacting with Bombyx mori IAP and Their Effects on BmNPV Proliferation [J]. Scientia Agricultura Sinica, 2019, 52(3): 558-567.
[13] ShaoFeng DENG,ZuoDong YE,ShuangQi FAN,JinDing CHEN,JingYuan ZHANG,MengJiao ZHU,MingQiu ZHAO. Screen of MicroRNAs in Classical Swine Fever Virus-Infected PK-15 Cells and the Regulation of Virus Replication by miR-214 [J]. Scientia Agricultura Sinica, 2018, 51(21): 4157-4168.
[14] CHEN Lin-lin, HOU Ying, DING Sheng-li, SHI Yan, LI Hong-lian. Cloning and Expression Analysis of Apoptosis-Related Gene FpTatD in Fusarium pseudograminearum [J]. Scientia Agricultura Sinica, 2016, 49(12): 2301-2309.
[15] PAN Yang-yang, LI Qin, CUI Yan, FAN Jiang-feng, YANG Kun, HE Jun-feng, YU Si-jiu. The Expression of EGF and EGFR in Yak Oocyte and Its Function on Development Competence of Embryo [J]. Scientia Agricultura Sinica, 2015, 48(12): 2439-2448.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd