FATP1对山羊肌内脂肪细胞的促进作用

doi:10.3864/j.issn.0578-1752.2023.10.015

Abstract

Abstract:

【Background】Fatty acid transporter 1 (FATP1) can promote the uptake of fatty acids in mammals. This process is very important to maintain the balance of lipid metabolism, and also has an important impact on the meat quality of livestock.【Objective】The aim of this study was to obtain the CDS sequence of goat FATP1 gene, to detect the expression of FATP1 gene in different tissues of goats, and to explore its effect on lipid metabolism of goat intramuscular adipocytes, so as to provide a reference for further revealing the mechanism of FATP1 gene in goat lipid metabolism, which can provide a theoretical basis for genetic and breeding improvement of goats.【Method】The CDS of goat FATP1 gene was cloned by real-time fluorescence quantitative PCR (RT-PCR), its biological characteristics, such as hydrophobicity, transmembrane region and signal peptide, were analyzed by online tools, and its amino acid sequence phylogenetic tree was constructed. The expression level of FATP1 gene in different goat tissues was detected by RT-qPCR and its tissue expression pattern was constructed. The constructed eukaryotic expression vector and screened siRNA were used to overexpress and interfere with FATP1 in goat intramuscular adipocytes, the effects of FATP1 gene overexpression and interference on lipid deposition in goat intramuscular adipocytes were detected by oil red O staining and triglyceride determination, and the effects of FATP1 gene overexpression and interference on the expression of genes related to lipid metabolism were further explored by RT-qPCR.【Result】The CDS of FATP1 gene was 1 941 bp, encoding 646 amino acids residues. It was predicted that its molecular formula was C₃₁₉₆H₅₀₂₆N₈₈₄O₈₉₈S₂₅, and the protein was a basic hydrophobic stable protein. Phylogenetic tree analysis of amino acid sequence showed that goat FATP1 was closely related to sheep. RT-qPCR showed that the expression of FATP1 gene was the highest in goat small intestine. Oil red O staining and triglyceride determination showed that the number of lipid droplets and triglyceride content in goat intramuscular adipocytes increased after overexpression of FATP1, but the opposite results were obtained after interference with FATP1. After overexpression of FATP1 in goat adipocytes, the expression levels of fatty acid synthesis, transport and other related genes AGPAT6(P<0.01), PLIN1(P<0.01), DGAT2(P<0.01), FADS2(P<0.01), FADS1(P<0.01), ACSL1(P<0.01) and ELOVL3 (P<0.05) increased significantly, while the expression level of lipolysis related genes ACOX1 (P<0.01) decreased significantly. After interfering FATP1, the expression of fatty acid transport, elongation and other related genes SCD5 (P<0.01), FABP3 (P<0.01) and ELOVL3 (P<0.05) decreased significantly, and the expression of lipolysis related genes ACOX1 (P<0.01) and CPT1B (P<0.05) increased significantly.【Conclusion】FATP1 might significantly promote the lipid deposition of goat intramuscular precursor adipocytes by promoting the expression of genes related to cell lipid production and reducing the expression of genes related to lipolysis, which provided an experimental reference for further revealing the role and molecular mechanism of FATP1 gene in regulating lipid metabolism.

Key words: goat, FATP1, intramuscular adipocytes, lipid deposition

LI Qi, YANG ChangHeng, WANG Yong, LIN YaQiu, XIANG Hua, ZHU JiangJiang. Role of FATP1 in Promoting Lipid Deposition in Goat Intramuscular Adipocytes[J].Scientia Agricultura Sinica, 2023, 56(10): 2007-2020.

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Figures/Tables 10

Table 1

Table 2

Primers for quantitative real-time PCR (RT-qPCR)"

基因 Gene	全称 Full name	序列 Sequence (5′-3′)	退火温度 Tm (℃)	产物长度 Products length (bp)	基因登录号 Genbank ID
FATP1	Fatty acid transport protein 1	S: ACCACTCGGCAGGGAACATC	60	108	本试验所得序列 The sequence obtained in this experiment
FATP1	Fatty acid transport protein 1	A: TGACGCAATCATCCCAGAAGC	60	108	本试验所得序列 The sequence obtained in this experiment
ACSL1	Acyl-CoA synthetase long-chain family member 1	S: TGACTGTTGCTGGAGACTGG	60	199	XM_005698718
ACSL1	Acyl-CoA synthetase long-chain family member 1	A: CAGCCGTCTTTATCCAGAGC	60	199	XM_005698718
ACSS2	Acyl-CoA synthetase short-chain family member 2	S: GGCGAATGCCTCTACTGCTT	60	100	XM_018057751
ACSS2	Acyl-CoA synthetase short-chain family member 2	A: GGCCAATCTTTTCTCTAATCTGCTT	60	100	XM_018057751
FABP3	Fatty acid binding protein 3	S: GATGAGACCACGGCAGATG	60	120	NM_001285701
FABP3	Fatty acid binding protein 3	A: GTCAACTATTTCCCGCACAAG	60	120	NM_001285701
SCD1	Stearoyl-CoA desaturase 1	S: CCATCGCCTGTGGAGTCAC	60	256	NM_001285619
SCD1	Stearoyl-CoA desaturase 1	A: GTCGGATAAATCTAGCGTAGCA	60	256	NM_001285619
SCD5	Stearoyl-CoA desaturase 5	S: CTGCTCTGGGCCTATTTCTG	60	165	XM_018049650
SCD5	Stearoyl-CoA desaturase 5	A: CCTCGACCACTCGAAGATGT	60	165	XM_018049650
FADS1	Fatty acid desaturase 1	S: GGTGGACTTGGCCTGGATG	60	101	XM_018043055
FADS1	Fatty acid desaturase 1	A: TGACCATGAAGACAAGCCCC	60	101	XM_018043055
FADS2	Fatty acid desaturase 2	S: GCCTGTAGGCTCAGATGTTTGTTC	60	101	XM_018043056
FADS2	Fatty acid desaturase 2	A: TGCCTGGCAGTAACAGAGCAC	60	101	XM_018043056
ELOVL3	ELOVL fatty acid elongase 3	S: CTGTCGGTATCCTGGCTTAT	55	151	XM_005698356
ELOVL3	ELOVL fatty acid elongase 3	A: GCTTTGATCTTGGGAATTATGT	55	151	XM_005698356
ELOVL6	ELOVL fatty acid elongase 6	S: GGAAGCCTTTAGTGCTCTGGTC	60	205	NM_001314257
ELOVL6	ELOVL fatty acid elongase 6	A: ATTGTATCTCCTAGTTCGGGTGC	60	205	NM_001314257
GPAM	Glycerol-3-phosphate acyltransferase, mitochondrial	S: GCAGGTTTATCCAGTATGGCATT	60	64	XM_013975269
GPAM	Glycerol-3-phosphate acyltransferase, mitochondrial	A: GGACTGATATCTTCCTGATCATCTTG	60	64	XM_013975269
AGPAT6	1-acylglycerol-3-phosphate O-acyltransferase 6	A: AAGCAAGTTGCCCATCCTCA	60	101	JI861797.1
AGPAT6	1-acylglycerol-3-phosphate O-acyltransferase 6	S: AAACTGTGGCTCCAATTTCGA	60	101	JI861797.1
LPIN1	Perilipin 1	S: CCCATTGCCAGCACTTCAGA	60	95	XM_018066570
LPIN1	Perilipin 1	A: GCAGCGTACTCGGCAGTATCTC	60	95	XM_018066570
DGAT1	Diacylglycerol O-acyltransferase 1	S: CCACTGGGACCTGAGGTGTC	60	101	XM_018058728
DGAT1	Diacylglycerol O-acyltransferase 1	A: GCATCACCACACACCAATTCA	60	101	XM_018058728
DGAT2	Diacylglycerol O-acyltransferase 2	S: CATGTACACATTCTGCACCGATT	60	100	NM_001314305
DGAT2	Diacylglycerol O-acyltransferase 2	A: TGACCTCCTGCCACCTTTCT	60	100	NM_001314305
ATGL	Adipose triglyceride lipase	S: GGAGCTTATCCAGGCCAATG	60	180	NM_001285739
ATGL	Adipose triglyceride lipase	A: TGCGGGCAGATGTCACTCT	60	180	NM_001285739
HSL	Hormone-sensitive lipase	S: TGCCCAAGACAGAGCCAATG	60	181	EU273879.1
HSL	Hormone-sensitive lipase	A: GCGGAGGAGCCGAGTATCT	60	181	EU273879.1
ACOX1	Acyl-CoA oxidase 1	S: CGAGTTCATTCTCAACAGTCCT	60	245	XM_018063771
ACOX1	Acyl-CoA oxidase 1	A: GCATCTTCAAGTAGCCATTATCC	60	245	XM_018063771
CPT1A	Carnitine palmitoyl transferase 1A	S: TGACGGCTCTGGCACAAGAT	60	164	XM_018043311
CPT1A	Carnitine palmitoyl transferase 1A	A: CGCGAAGTAGTTGCTATTCAC	60	164	XM_018043311
CPT1B	Carnitine palmitoyl transferase 1B	S: ACGAGGAGTCTCACCACTACG	60	111	XM_018048994
CPT1B	Carnitine palmitoyl transferase 1B	A: GTGTGAAGGACTTGTCGAACCA	60	111	XM_018048994
UXT	Ubiquitously expressed transcript	S: GCAAGTGGATTTGGGCTGTAAC	60	180	XM_005700842
UXT	Ubiquitously expressed transcript	A: ATGGAGTCCTTGGTGAGGTTGT	60	180	XM_005700842

Table 2

Table 3

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Fig. 2

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References 49

[1]	STEFAN N, SCHICK F, HÄRING H U. Causes, characteristics, and consequences of metabolically unhealthy normal weight in humans. Cell Metabolism, 2017, 26(2): 292-300. doi: S1550-4131(17)30429-1 pmid: 28768170
[2]	张龙, 朱方俊, 王彦, 兰茜, 吕荣平, 刘益平, 朱庆. 鸡FATP1基因多态性与屠体性状的相关分析. 中国农业科学, 2009, 42(9): 3272-3278. doi: 10.3864/j.issn.0578-1752.2009.09.033
	ZHANG L, ZHU F J, WANG Y, LAN X, LÜ R P, LIU Y P, ZHU Q. Correlation analysis between FATP1 gene polymorphism and carcass traits in chicken. Scientia Agricultura Sinica, 2009, 42(9): 3272-3278. (in Chinese)
[3]	华绪川, 张立凡, 蔡兆伟, 蒋晓玲, 徐宁迎, 张金枝. 猪FATP1基因5’调控区多态性及其与脂肪性状的相关分析. 江苏农业学报, 2011, 27(1): 89-93.
	HUA X C, ZHANG L F, CAI Z W, JIANG X L, XU N Y, ZHANG J Z. Polymorphism in 5’regulatory region of Porcine FATP1 gene and its association with fat traits. Jiangsu Journal of Agricultural Sciences, 2011, 27(1): 89-93. (in Chinese)
[4]	黄业传, 贺稚非, 李洪军, 秦刚, 王庭, 马明辉. 皮下脂肪和肌内脂肪对猪肉风味的作用. 中国农业科学, 2011, 44(10): 2118-2130. doi:10.3864/j.issn.0578-1752.2011.10.017. doi: 10.3864/j.issn.0578-1752.2011.10.017
	HUANG Y C, HE Z F, LI H J, QIN G, WANG T, MA M H. The flavor contribution of subcutaneous and intramuscular fat to pork. Scientia Agricultura Sinica, 2011, 44(10): 2118-2130. doi:10.3864/j.issn.0578-1752.2011.10.017. (in Chinese) doi: 10.3864/j.issn.0578-1752.2011.10.017
[5]	STAHL A, EVANS J G, PATTEL S, HIRSCH D, LODISH H F. Insulin causes fatty acid transport protein translocation and enhanced fatty acid uptake in adipocytes. Developmental Cell, 2002, 2(4): 477-488. pmid: 11970897
[6]	GIMENO R E. Fatty acid transport proteins. Current Opinion in Lipidology, 2007, 18(3): 271-276. pmid: 17495600
[7]	KAZANTZIS M, STAHL A. Fatty acid transport proteins, implications in physiology and disease. Biochimica et Biophysica Acta, 2012, 1821(5): 852-857. doi: 10.1016/j.bbalip.2011.09.010 pmid: 21979150
[8]	POHL J, RING A, HERMANN T, STREMMEL W. Role of FATP in parenchymal cell fatty acid uptake. Biochimica et Biophysica Acta, 2004, 1686(1/2): 1-6.
[9]	WU Q W, ORTEGON A M, TSANG B, DOEGE H, FEINGOLD K R, STAHL A. FATP1 is an insulin-sensitive fatty acid transporter involved in diet-induced obesity. Molecular and Cellular Biology, 2006, 26(9): 3455-3467. doi: 10.1128/MCB.26.9.3455-3467.2006 pmid: 16611988
[10]	SCHAFFER J E, LODISH H F. Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell, 1994, 79(3): 427-436. doi: 10.1016/0092-8674(94)90252-6 pmid: 7954810
[11]	GUITART M, OSORIO-CONLES O, PENTINAT T, CEBRIÀ J, GARCÍA-VILLORIA J, SALA D, SEBASTIÁN D, ZORZANO A, RIBES A, JIMÉNEZ-CHILLARÓN J C, GARCÍA-MARTÍNEZ C, GÓMEZ-FOIX A M. Fatty acid transport protein 1 (FATP1) localizes in mitochondria in mouse skeletal muscle and regulates lipid and ketone body disposal. PLoS ONE, 2014, 9(5): e98109. doi: 10.1371/journal.pone.0098109
[12]	ORDOVÁS L, ROY R, ZARAGOZA P, RODELLAR C. Structural and functional characterization of the bovine solute carrier family 27 member 1 (SLC27A1) gene. Cytogenetic and Genome Research, 2006, 115(2): 115-122. pmid: 17065791
[13]	LEWIS S E, LISTENBERGER L L, ORY D S, SCHAFFER J E. Membrane topology of the murine fatty acid transport protein 1. Journal of Biological Chemistry, 2001, 276(40): 37042-37050. doi: 10.1074/jbc.M105556200 pmid: 11470793
[14]	CHEN X L, LUO Y L, WANG R S, ZHOU B, HUANG Z Q, JIA G, ZHAO H, LIU G M. Effects of fatty acid transport protein 1 on proliferation and differentiation of porcine intramuscular preadipocytes. Animal Science Journal, 2017, 88(5): 731-738. doi: 10.1111/asj.12701 pmid: 27616431
[15]	WANG H, WANG J, YANG D D, LIU Z L, ZENG Y Q, CHEN W. Expression of lipid metabolism genes provides new insights into intramuscular fat deposition in Laiwu pigs. Asian-Australasian Journal of Animal Sciences, 2020, 33(3): 390-397. doi: 10.5713/ajas.18.0225 pmid: 31480195
[16]	COE N R, SMITH A J, FROHNERT B I, WATKINS P A, BERNLOHR D A. The fatty acid transport protein (FATP1) is a very long chain acyl-CoA synthetase. Journal of Biological Chemistry, 1999, 274(51): 36300-36304. doi: 10.1074/jbc.274.51.36300 pmid: 10593920
[17]	HALL A M, SMITH A J, BERNLOHR D A. Characterization of the acyl-CoA synthetase activity of purified murine fatty acid transport protein 1. Journal of Biological Chemistry, 2003, 278(44): 43008-43013. doi: 10.1074/jbc.M306575200 pmid: 12937175
[18]	HALL A M, WICZER B M, HERRMANN T, STREMMEL W, BERNLOHR D A. Enzymatic properties of purified murine fatty acid transport protein 4 and analysis of acyl-CoA synthetase activities in tissues from FATP4 null mice. Journal of Biological Chemistry, 2005, 280(12): 11948-11954. doi: 10.1074/jbc.M412629200 pmid: 15653672
[19]	HEATHER L C, COLE M A, LYGATE C A, EVANS R D, STUCKEY D J, MURRAY A J, NEUBAUER S, CLARKE K. Fatty acid transporter levels and palmitate oxidation rate correlate with ejection fraction in the infarcted rat heart. Cardiovascular Research, 2006, 72(3): 430-437. doi: 10.1016/j.cardiores.2006.08.020 pmid: 17034771
[20]	ZHANG M M, DI MARTINO J S, BOWMAN R L, CAMPBELL N R, BAKSH S C, SIMON-VERMOT T, KIM I S, HALDEMAN P, MONDAL C, YONG-GONZALES V, ABU-AKEEL M, MERGHOUB T, JONES D R, ZHU X G, ARORA A, ARIYAN C E, BIRSOY K, WOLCHOK J D, PANAGEAS K S, HOLLMANN T, BRAVO-CORDERO J J, WHITE R M. Adipocyte-derived lipids mediate melanoma progression via FATP proteins. Cancer Discovery, 2018, 8(8): 1006-1025. doi: 10.1158/2159-8290.CD-17-1371 pmid: 29903879
[21]	ZHAN T Z, POPPELREUTHER M, EHEHALT R, FÜLLEKRUG J. Overexpressed FATP1, ACSVL4/FATP4 and ACSL1 increase the cellular fatty acid uptake of 3T3-L1 adipocytes but are localized on intracellular membranes. PLoS ONE, 2012, 7(9): e45087. doi: 10.1371/journal.pone.0045087
[22]	STAHL A. A current review of fatty acid transport proteins (SLC27). Pflügers Archiv, 2004, 447(5): 722-727. doi: 10.1007/s00424-003-1106-z
[23]	DOEGE H, STAHL A. Protein-mediated fatty acid uptake: novel insights from in vivo models. Physiology (Bethesda, Md), 2006, 21: 259-268.
[24]	STEFANYK L E, BONEN A, DYCK D J. Insulin and contraction- induced movement of fatty acid transport proteins to skeletal muscle transverse-tubules is distinctly different than to the sarcolemma. Metabolism, 2012, 61(11): 1518-1522. doi: 10.1016/j.metabol.2012.04.002
[25]	WICZER B M, LOBO S, MACHEN G L, GRAVES L M, BERNLOHR D A. FATP1 mediates fatty acid-induced activation of AMPK in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications, 2009, 387(2): 234-238. doi: 10.1016/j.bbrc.2009.06.114 pmid: 19560442
[26]	GARCÍA-MARTÍNEZ C, MAROTTA M, MOORE-CARRASCO R, GUITART M, CAMPS M, BUSQUETS S, MONTELL E, GÓMEZ-FOIX A M. Impact on fatty acid metabolism and differential localization of FATP1 and FAT/CD36 proteins delivered in cultured human muscle cells. American Journal of Physiology Cell Physiology, 2005, 288(6): C1264-C1272. doi: 10.1152/ajpcell.00271.2004
[27]	KIM J K, GIMENO R E, HIGASHIMORI T, KIM H J, CHOI H, PUNREDDY S, MOZELL R L, TAN G, STRICKER-KRONGRAD A, HIRSCH D J, FILLMORE J J, LIU Z X, DONG J Y, CLINE G, STAHL A, LODISH H F, SHULMAN G I. Inactivation of fatty acid transport protein 1 prevents fat-induced insulin resistance in skeletal muscle. The Journal of Clinical Investigation, 2004, 113(5): 756-763. doi: 10.1172/JCI200418917
[28]	池永东, 王永, 胡萌, 何小芳, 朱江江, 赵越, 林亚秋. 山羊不同组织器官的内参基因筛选. 基因组学与应用生物学, 2020, 39(2): 561-567.
	CHI Y D, WANG Y, HU M, HE X F, ZHU J J, ZHAO Y, LIN Y Q. Screening of internal reference genes in different tissues and organs of goats. Genomics and Applied Biology, 2020, 39(2): 561-567. (in Chinese)
[29]	许晴, 林森, 朱江江, 王永, 林亚秋. 山羊肌内前体脂肪细胞诱导分化过程中内参基因的表达稳定性分析. 畜牧兽医学报, 2018, 49(5): 907-918.
	XU Q, LIN S, ZHU J J, WANG Y, LIN Y Q. The expression stability analysis of reference genes in the process of goat intramuscular preadipocytes differentiation in goat. Chinese Journal of Animal and Veterinary Sciences, 2018, 49(5): 907-918. (in Chinese)
[30]	蔡勇, 阿依木古丽, 臧荣鑫, 刘翊中, 杨具田, 乔自林, 曹忻, 徐红伟, 吴建平. 不同冻存保护剂对绵羊前体脂肪细胞冻存效果的影响. 中国农业科学, 2012, 45(7): 1439-1446. doi: 10.3864/j.issn.0578-1752.2012.07.024
	CAI Y, AYIMUGULI, ZANG R X, LIU Y Z, YANG J T, QIAO Z L, CAO X, XU H W, WU J P. Effects of different cryoprotectants on ovine preadipocytes cryopreservation. Scientia Agricultura Sinica, 2012, 45(7): 1439-1446. (in Chinese) doi: 10.3864/j.issn.0578-1752.2012.07.024
[31]	HATCH G M, SMITH A J, XU F Y, HALL A M, BERNLOHR D A. FATP1 channels exogenous FA into 1, 2, 3-triacyl-sn-glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cells. Journal of Lipid Research, 2002, 43(9): 1380-1389. doi: 10.1194/jlr.M200130-JLR200
[32]	WU Q W, KAZANTZIS M, DOEGE H, ORTEGON A M, TSANG B, FALCON A, STAHL A. Fatty acid transport protein 1 is required for nonshivering thermogenesis in brown adipose tissue. Diabetes, 2006, 55(12): 3229-3237. pmid: 17130465
[33]	QI R L, LONG D B, WANG J, WANG Q, HUANG X F, CAO C T, GAO G L, HUANG J X. MicroRNA-199a targets the fatty acid transport protein 1 gene and inhibits the adipogenic trans- differentiation of C2C12 myoblasts. Cellular Physiology and Biochemistry: International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 2016, 39(3): 1087-1097. doi: 10.1159/000447817
[34]	DIRUSSO C C, LI H, DARWIS D, WATKINS P A, BERGER J, BLACK P N. Comparative biochemical studies of the murine fatty acid transport proteins (FATP) expressed in yeast. Journal of Biological Chemistry, 2005, 280(17): 16829-16837. doi: 10.1074/jbc.M409598200 pmid: 15699031
[35]	LOBO S, WICZER B M, SMITH A J, HALL A M, BERNLOHR D A. Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4. Journal of Lipid Research, 2007, 48(3): 609-620. doi: 10.1194/jlr.M600441-JLR200 pmid: 17164224
[36]	QIU F F, XIE L, MA J G, LUO W, ZHANG L, CHAO Z, CHEN S H, NIE Q H, LIN Z M, ZHANG X Q. Lower expression of SLC27A1 enhances intramuscular fat deposition in chicken via down-regulated fatty acid oxidation mediated by CPT1A. Frontiers in Physiology, 2017, 8: 449. doi: 10.3389/fphys.2017.00449
[37]	NICKERSON J G, ALKHATEEB H, BENTON C R, LALLY J, NICKERSON J, HAN X X, WILSON M H, JAIN S S, SNOOK L A, GLATZ J F C, CHABOWSKI A, LUIKEN J J F P, BONEN A. Greater transport efficiencies of the membrane fatty acid transporters FAT/CD36 and FATP4 compared with FABPpm and FATP1 and differential effects on fatty acid esterification and oxidation in rat skeletal muscle. Journal of Biological Chemistry, 2009, 284(24): 16522-16530. doi: 10.1074/jbc.M109.004788 pmid: 19380575
[38]	SEBASTIÁN D, GUITART M, GARCÍA-MARTÍNEZ C, MAUVEZIN C, ORELLANA-GAVALDÀ J M, SERRA D, GÓMEZ-FOIX A M, HEGARDT F G, ASINS G. Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells. Journal of Lipid Research, 2009, 50(9): 1789-1799. doi: 10.1194/jlr.M800535-JLR200 pmid: 19429947
[39]	CHIU H C, KOVACS A, BLANTON R M, HAN X L, COURTOIS M, WEINHEIMER C J, YAMADA K A, BRUNET S, XU H D, NERBONNE J M, WELCH M J, FETTIG N M, SHARP T L, SAMBANDAM N, OLSON K M, ORY D S, SCHAFFER J E. Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy. Circulation Research, 2005, 96(2): 225-233. doi: 10.1161/01.RES.0000154079.20681.B9
[40]	HUANG J P, ZHU R R, SHI D S. The role of FATP1 in lipid accumulation: a review. Molecular and Cellular Biochemistry, 2021, 476(4): 1897-1903. doi: 10.1007/s11010-021-04057-w pmid: 33486652
[41]	HOLLOWAY G P, CHOU C J, LALLY J, STELLINGWERFF T, MAHER A C, GAVRILOVA O, HALUZIK M, ALKHATEEB H, REITMAN M L, BONEN A. Increasing skeletal muscle fatty acid transport protein 1 (FATP1) targets fatty acids to oxidation and does not predispose mice to diet-induced insulin resistance. Diabetologia, 2011, 54(6): 1457-1467. doi: 10.1007/s00125-011-2114-8 pmid: 21442160
[42]	FROHNERT B I, HUI T Y, BERNLOHR D A. Identification of a functional peroxisome proliferator-responsive element in the murine fatty acid transport protein gene. Journal of Biological Chemistry, 1999, 274(7): 3970-3977. doi: 10.1074/jbc.274.7.3970 pmid: 9933587
[43]	YE G Z, GAO H, WANG Z C, LIN Y, LIAO X, ZHANG H, CHI Y L, ZHU H M, DONG S J. PPARα and PPARγ activation attenuates total free fatty acid and triglyceride accumulation in macrophages via the inhibition of Fatp1 expression. Cell Death & Disease, 2019, 10: 39.
[44]	CHOI H, KIM S J, PARK S S, CHANG C, KIM E. TR4 activates FATP1 gene expression to promote lipid accumulation in 3T3-L1 adipocytes. FEBS Letters, 2011, 585(17): 2763-2767. doi: 10.1016/j.febslet.2011.08.002
[45]	PROSDOCIMO D A, ANAND P, LIAO X D, ZHU H, SHELKAY S, ARTERO-CALDERON P, ZHANG L L, KIRSH J, MOORE D, ROSCA M G, VAZQUEZ E, KERNER J, AKAT K M, WILLIAMS Z, ZHAO J H, FUJIOKA H, TUSCHL T, BAI X D, SCHULZE P C, HOPPEL C L, JAIN M K, HALDAR S M. Kruppel-like factor 15 is a critical regulator of cardiac lipid metabolism. Journal of Biological Chemistry, 2014, 289(9): 5914-5924. doi: 10.1074/jbc.M113.531384 pmid: 24407292
[46]	ZHAO Z D, TIAN H S, SHI B G, JIANG Y Y, LIU X, HU J. Transcriptional regulation of the bovine fatty acid transport protein 1 gene by Krüppel-like factors 15. Animals: an Open Access Journal from MDPI, 2019, 9(9): 654.
[47]	朱江江, 林亚秋, 王永, 林森. 山羊Kruppel样转录因子家族在前体脂肪细胞分化中的表达模式及相关性分析. 中国农业科学, 2019, 52(13): 2341-2351. doi:10.3864/j.issn.0578-1752.2019.13.012. doi: 10.3864/j.issn.0578-1752.2019.13.012
	ZHU J J, LIN Y Q, WANG Y, LIN S. Expression profile and correlations of kruppel like factors during caprine(Capra hircus) preadipocyte differentiation. Scientia Agricultura Sinica, 2019, 52(13): 2341-2351. doi:10.3864/j.issn.0578-1752.2019.13.012. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.13.012
[48]	MAK H Y. Lipid droplets as fat storage organelles in Caenorhabditis elegans: thematic Review Series: Lipid Droplet Synthesis and Metabolism: from Yeast to Man. Journal of Lipid Research, 2012, 53(1): 28-33. doi: 10.1194/jlr.R021006
[49]	XU N Y, ZHANG S O, COLE R A, MCKINNEY S A, GUO F L, HAAS J T, BOBBA S, FARESE R V Jr, MAK H Y. The FATP1-DGAT2 complex facilitates lipid droplet expansion at the ER-lipid droplet interface. The Journal of Cell Biology, 2012, 198(5): 895-911. doi: 10.1083/jcb.201201139

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基因 Gene	序列 Sequence (5′-3′)	退火温度 Tm (℃)	产物长度 Products length (bp)	用途 Purpose
FATP1	S: CAAAGCCTGAGGATCTGTGAG	64-55	2078	克隆 Clone
FATP1	A: GGAGTCTTGGGCCAACACA	64-55	2078	克隆 Clone
FATP1	S: CCCAAGCTTGCCACCATGGATTACAAGGAT′ GACGACGATAAGCGGGCTCCAGGTGCAGGT	66-56	1980	亚克隆 Subclone
FATP1	A: CGCGGATCCTCAGAGGGCGAAGGCGCCTGA	66-56	1980	亚克隆 Subclone
S：正义链引物；A：反义链引物。斜体为保护碱基，为酶切位点，为kozak序列，为Flag标签序列 S: Sense primer; A: Antisense primer. Italics are protective bases, enzyme digestion sites, Kozak sequences, Flag tag sequences

基因 Gene	序列 Sequence (5′-3′)
siRNA-NC	UUCUCCGAACGUGUCACGUTT
siRNA-NC	ACGUGACACGUUCGGAGAATT
siRNA-FATP1-1	GGGUCAGUGUCUCAUCUAUTT
siRNA-FATP1-1	AUAGAUGAGACACUGACCCTT
siRNA-FATP1-2	GUGCCACGAACAAGAAGAUTT
siRNA-FATP1-2	AUCUUCUUGUUCGUGGCACTT
siRNA-FATP1-3	CAGGCACCUUCAAGAUUCATT
siRNA-FATP1-3	UGAAUCUUGAAGGUGCCUGTT

Role of FATP1 in Promoting Lipid Deposition in Goat Intramuscular Adipocytes

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