牛源金黄色葡萄球菌的耐药性及耐甲氧西林金黄色葡萄球菌的检测

doi:10.3864/j.issn.0578-1752.2012.17.017

Abstract

Abstract: 【Objective】The aim of the study is to investigate the antimicrobial resistance of S. aureus (Science) and to detect the presence of methicillin-resistant S.aureus (MRSA) among S.aureus strains isolated from bovine mastitis in Inner Mongolia, and to provide credible theory evidence for prevention and treatment of bovine mastitis. 【Method】 K-B disk diffusion method was used to test drug sensitivity of 38 total strains of S.aureus to 17 commonly used antibiotics. Meanwhile, agar screen was performed to identify the minimum inhibitory concentration of oxacillin and vancomycin to all strains. Cefoxitin, oxacillin disk diffusion and oxacillin agar screen were used to detect the phenotype of MRSA, and PCR assay was generated the genotype of MRSA as well. 【Result】 The isolates had different degrees of antimicrobial resistance to each antibiotic, the frequency of resistance to ampicillin, cefradine, penicillin, cotrimoxazole, novobiocin and streptomycin was more than 45%, yet keeping sensitive to ofloxacin, vancomycin, amikacin, ciprofloxacin, gentamicin and cefazolin was over 90%. Two of the strains with vancomycin MIC were ≥16 μg•mL-1. The MICs of oxacillin for eight of total strains were ≥ 8 μg•mL-1, others with oxacillin MICs were ≤2 μg•mL-1. The multidrug resistance was severe, 84.21% of the strains were resistant to at least 3 kinds of antimicrobial agent, four of the total strains can survive in the presence of night various antibiotics. 16(42.11%) S.aureus strains carried mecA gene detected by PCR assay. However, there were only seven of them have the minimum inhibitory concentration over 4 μg•mL-1. When cefoxitin, oxacillin disk diffusion and oxacillin agar screen methods were generated the phenotype of MRSA, only 7, 10 and 7 strains of each were identified. 【Conclusion】 The antimicrobial resistance and multidrug resistance of S aureus were serious. High incidence of MRSA and OS-MRSA has been associated with bovine mastitis in the surveyed region.

Key words: Staphylococcus aureus, MRSA, antimicrobial resistance, mecA gene, bovine mastitis

SU Yang-., PU Wan-Xia, CHEN Zhi-Hua, DENG Hai-Ping. Antimicrobial Resistance Analysis and Detection of Methicillin- Resistant Staphylococcus aureus (MRSA) Among Staphylococcus aureus Strains Isolated from Bovine Mastitis[J].Scientia Agricultura Sinica, 2012, 45(17): 3602-3607.

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Antimicrobial Resistance Analysis and Detection of Methicillin- Resistant Staphylococcus aureus (MRSA) Among Staphylococcus aureus Strains Isolated from Bovine Mastitis

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