Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (22): 4154-4165.doi: 10.3864/j.issn.0578-1752.2019.22.019

• CULTIVATION·PHYSIOLOGY • Previous Articles     Next Articles

Effect of Sowing Date on Agronomic Traits and Starch Physicochemical Properties of Proso Millet

LUO Yan1,QU Yang2,YANG QingHua1,ZHANG WeiLi1,GONG XiangWei1,LI Jing1,GAO XiaoLi1,GAO JinFeng1,YANG Pu1,WANG PengKe1,FENG BaiLi1()   

  1. 1 College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Research Station of Crop Gene Resources & Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, Shaanxi;
    2 Baoji Academy of Agricultural Sciences, Baoji 722400, Shaanxi
  • Received:2019-06-14 Accepted:2019-09-12 Online:2019-11-16 Published:2019-11-16
  • Contact: BaiLi FENG E-mail:fengbaili@nwsuaf.edu.cn

Abstract:

【Objective】 The purpose of this study was to analyze the effects of different sowing date on proso millet (Panicum miliaceum L.) agronomic traits and grain starch physiochemical properties, provide theoretical basis for the subsequent study of the mechanism of sowing date on proso millet.【Method】The experiment was carried out in a pot, and Shaanmei No. 1 was used as the test material. Four kinds of sowing dates were set on April 20 (B1), May 10 (B2), May 30 (B3), and June 20 (B4). Different sowing dates, the soil moisture was sufficient during the whole growth period. In 2017, the morphological indexes, grain shape and grain physicochemical properties of the seeds of the proso millet were studied. The plant height, ear length, ear stem length, number of ear branches, 1 000-grain weight, the seeds length, width and roundness of proso millet were analyzed under different sowing dates. In the meantime, the crystal structure, particle size distribution, amylose content, and the enthalpy and gelatinization properties of starch were studied. The correlation of amylose content, enthalpy characteristics and gelatinization properties of starch with morphological index, particle size distribution and particle size parameters were analyzed. 【Result】 The results showed that with the delay of sowing date, the plant height, ear length and ear stem length of proso millet were significantly increased, the main stem tillers and branches were significantly decreased, and the number of secondary spike branches and grain width were significantly decreased. The size distribution of grain starch granules showed a bimodal distribution, and compared with other sowing dates, the particles proportion of B1 had the highest > 28 μm (25.5%) and the lowest 5-28 μm (67.5%) starch granule. The X-ray diffraction pattern showed that changing the sowing date couldn’t affect the crystal type of the proso millet starch, but affected the diffraction peak intensity of the X-ray diffraction pattern. The amylose content (1.3%) of B1 was significantly higher than that in the other sowing dates (0.18%-0.53%). Among the indicators related to the enthalpy characteristics of grain starch in different sowing dates, the gelatinization onset temperature (To), peak temperature (Tp) and conclusion temperature (Tc) showed a tendency of increasing first and then decreasing, while the thermal enthalpy (ΔH) had no significant difference. There were significant differences in the gelatinization properties of proso millet starch under different sowing dates. The peak viscosity (Pv), through viscosity (Tv), final viscosity (Fv) and breakdown (BD) of B3 were significantly higher than the others, while the setback (SB) and peak temperature (PT) were significantly lower than other treatments. With the delay of sowing date, the Pv and BD of proso millet starch increased first and then decreased. B1 starch had the strongest hot paste stability, B3 starch had the strongest cold paste stability and the worst hot paste stability. The cold paste stability of B2 starch was the worst, while the cold paste and hot paste stability of B4 were at an intermediate level. 【Conclusion】 Appropriate sowing date could provide sufficient light and temperature conditions to provide a better environment for the growth of proso millet. Early sowing date significantly increased the 1 000-grain weight of proso millet kernels, the grain was fuller, and the average grain size of grain starch increased, with higher amylose content and nutritional value, which was more suitable for processing hot foods. The sowing date didn’t affect the crystal type of starch, but affected the intensity of the X-ray diffraction peak, the gelatinization characteristics and other indicators of the starch.

Key words: sowing date, Panicum miliaceum L., starch, gelatinization characteristics, crystal structure

Fig. 1

Daily rainfall and mean temperature in growth period of the experiment station"

Table 1

Plant morphological indexes and grain shape questionnaire"

处理
Treatment
株高
Height
(cm)
分蘖
Bifurcation
分枝
Branch
穗长
Ear length
(cm)
穗柄长
Ear stem
length (cm)
茎粗
Stem thick
(cm)
节数
Section
number
千粒重
1 000-grain
weight (g)
穗分枝 Spike branch 籽粒性状 Grain properties
一级
Firstly
二级
Secondly
粒长
Length (mm)
粒宽
Width (mm)
长/宽
Length/width
圆度
Roundness
B1 82.27±12.900C 2.3±0.58A 1.0±0.00B 28.67±3.215C 22.73±5.354C 4.34±1.244a 3.3±1.53b 7.430±0.0198A 20.0±2.00ab 181.3±13.61A 3.4155±0.0078a 2.4425±0.0776a 1.4145±0.0516a 0.738±0.0226a
B2 126.67±8.505B 0.0±0.00B 2.0±1.00A 37.633±2.868B 30.40±1.970B 5.23±0.824a 6.0±0.00a 6.781±0.1164B 22.3±1.53a 120.7±9.23B 3.3950±0.0028a 2.3370±0.0240ab 1.4635±0.0134a 0.713±0.0071ab
B3 116.00±5.291B 0.0±0.00B 2.0±1.00A 38.30±2.117B 32.67±2.843B 4.24±0.038a 4.3±0.58ab 7.245±0.1272A 19.0±1.73b 109.0±16.64B 3.3975±0.0134a 2.4320±0.0099ab 1.4030±0.0014a 0.744±0.0014a
B4 146.33±6.351A 0.3±0.58B 0.0±0.00C 43.33±1.155A 44.33±1.527A 5.64±0.384a 5.7±0.58a 6.849±0.1166B 19.3±0.58ab 121.7±16.50B 3.4090±0.0085a 2.3215±0.0049b 1.4760±0.001a 0.702±0.0010b
F 28.118 22.667 11.000 18.325 22.332 2.384 6.074 26.695 2.805 15.573 2.328 4.754 3.618 5.677
P 0.000 0.000 0.003 0.001 0.000 0.145 0.019 0.000 0.108 0.001 0.216 0.083 0.123 0.063

Fig. 2

Starch size distribution map"

Table 2

Particle size parameters of proso millet grain starch under different sowing dates"

处理
Treatment
粒径范围 Grain size (%) 体积平均粒径D[4,3]
Volume average particle size (μm)
表面积平均粒径D[3,2]
Surface area average particle size (μm)
中值粒径d(0.5)
Median particle size (μm)
比表面积
Specific surface area
<5 μm 5—28 μm >28 μm
B1 6.96 67.52 25.52 24.990 10.972 12.188 0.439
B2 10.56 80.98 8.48 15.942 8.893 9.85 0.558
B3 6.35 83.85 9.80 19.941 10.236 11.421 0.513
B4 10.06 81.43 8.51 16.122 8.917 9.771 0.553

Fig. 3

Polarized cross of proso millet starch under different sowing dates A: Represents proso millet starch of B1; B: Represents proso millet starch of B2; C: Represents proso millet starch of B3; D: Represents proso millet starch of B4"

Fig. 4

X-ray diffraction pattern of proso millet"

Table 3

Thermal enthalpy characteristics of starch granules under different sowing dates"

处理 Treatment 起始温度 To (℃) 峰值温度 Tp (℃) 终值温度 Tc (℃) 热焓值 ΔH (J·g-1)
B1 68.31±3.51ab 75.84±3.56A 82.67±6.41BC 6.740±1.650a
B2 72.80±2.47a 79.17±0.17A 94.04±1.75A 7.510±0.461a
B3 73.63±1.48a 78.62±1.37A 90.51±1.30AB 6.496±0.347a
B4 65.27±5.67b 69.31±3.23B 78.39±4.86C 5.564±2.364a
F 3.501 9.827 8.812 0.0893
P 0.069 0.005 0.006 0.486

Table 4

Gelatinization characteristics of starch granules under different sowing dates"

处理
Treatment
峰值黏度
Pv (cP)
谷值黏度
Tv (cP)
终值黏度
Fv (cP)
破损值
BD (cP)
回生值
SB (cP)
糊化温度
PT (℃)
B1 1380.00±77.324B 938.67±40.067B 1068.00±58.284ab 441.33±38.940C 129.33±21.127B 83.30±0.100a
B2 1286.00±36.166B 734.67±19.858C 982.67±11.015b 551.33±16.503B 248.00±9.539A 83.30±0.000a
B3 1936.87±32.316A 1035.33±14.503A 1109.67±13.204a 901.33±19.399A 74.33±2.517C 82.61±0.023b
B4 1397.67±83.811B 895.67±87.672B 1032.00±88.504ab 502.00±35.384B 136.33±4.726B 83.52±0.548a
F 68.078 19.061 3.029 149.775 112.419 5.951
P 0.000 0.001 0.093 0.000 0.000 0.020

Table 5

Correlation between physicochemical properties of starch granules and starch particle size parameters"

相关性
Correlation
体积平均粒径D[4,3]
Volume average particle size
表面积平均粒径D[3,2]
Surface area average particle size
中值粒径d(0.5)
Median particle size
比表面积
Specific surface area
直链淀粉含量
Amylose content
< 5 μm 5-28 μm > 28 μm
峰值黏度 Pv 0.153 0.349 0.370 -0.084 -0.086 -0.690 0.417 -0.214
谷值黏度 Tv 0.527 0.649 0.646 -0.486 0.432 -0.827 -0.049 0.240
破损值 BD -0.098 0.111 0.143 0.173 -0.385 -0.497 0.633 -0.455
终值黏度 Fv 0.696 0.810 0.811 -0.653 0.545 -0.942 -0.214 0.411
回生值 SB -0.386 -0.509 -0.503 0.348 -0.335 0.712 -0.070 -0.109
糊化温度 PT -0.036 -0.240 -0.266 -0.037 0.222 0.607 -0.525 0.331
起始温度 To -0.482 -0.361 -0.316 0.533 -0.780 0.116 0.653 -0.624
峰值温度 Tp 0.355 0.394 0.431 -0.321 0.026 -0.380 -0.222 0.271
终值温度 Tc -0.156 -0.083 -0.038 0.197 -0.481 -0.025 0.260 -0.242
热焓值 ΔH 0.677 0.814 0.830 -0.620 0.420 -0.978* -0.153 0.360
直链淀粉含量
Amylose content
0.919 0.837 0.811 -0.944 1.000 -0.586 -0.915 0.958*

Table 6

Correlation between enthalpy characteristics and gelatinization properties of starch granules"

相关性
Correlation
峰值黏度
Pv
谷值黏度
Tv
破损值
BD
终值黏度
Fv
回生值
SB
糊化温度
PT
起始温度
To
峰值温度
Tp
终值温度
Tc
谷值黏度 Tv 0.807**
破损值 BD 0.925** 0.524
终值黏度 Fv 0.720** 0.907 ** 0.457
回生值 SB -0.744** -0.906** -0.492 -0.644*
糊化温度 PT -0.838** -0.657* -0.788** -0.742** 0.449
起始温度 To 0.302 -0.151 0.533 -0.176 0.098 -0.271
峰值温度 Tp 0.273 -0.104 0.462 0.031 0.220 -0.415 0.790**
终值温度 Tc 0.172 -0.297 0.438 -0.219 0.319 -0.436 0.546 0.700*
热焓值 ΔH -0.025 0.053 -0.071 -0.256 -0.353 0.459 -0.227 -0.520 -0.380

Fig. 5

Amylose content of proso millet grains under different sowing dates Uppercase letters among different treatments within each column are significantly different at 0.01 level"

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