Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (13): 2553-2563.doi: 10.3864/j.issn.0578-1752.2017.13.014

• HORTICULTURE • Previous Articles     Next Articles

The Difference of Anthocyanin Accumulation Pattern and Related Gene Expression in Two Kinds of Red Flesh Peach

DING TiYu, CAO Ke, FANG WeiChao, ZHU GengRui, CHEN ChangWen, WANG XinWei, WANG LiRong   

  1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009
  • Received:2017-01-03 Online:2017-07-01 Published:2017-07-01

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Abstract: 【Objective】The objective of this experiment is to investigate the coloring mechanism of Chinese red-flesh peach germplasms and lay a theoretical foundation for excellent gene identifying and molecular marker assisted breeding in blood-flesh peach accessions.【Method】Eight red flesh peaches and one white flesh peach were  selected as samples. The samples were collected one month after full bloom, and then collected every 10 days until the mature period. The anthocyanin of pulp of different germplasms was extracted with 2% formic acid methanol, determining the absorbance at 510 nm and 700 nm. The expression of 13 key structural genes and regulatory genes associated with anthocyanin synthesis was determined by real-time quantitative PCR (qRT-PCR).【Result】According to the accumulation of anthocyanin at middle-late stages of fruit, eight red-flesh peaches could be divided into two categories. The peak of anthocyanin biosynthesis appeared at mature stage, which was referred to as mature period accumulation type, including Zheng Yin 82-9, Da Hong Pao, Hei Bu Dai, Hong Tao and Tianjin Shui Mi; The peak of anthocyanin synthesis occurred at the middle stage of fruit development, and the content of anthocyanin decreased at mature stage, which was referred to as the mid-developmental accumulation type, including Harrow Blood, Da Guo Hei Tao and Wu Hei Ji Rou Tao. In the structural genes associated with anthocyanin, the expression of PpCHS and PpUFGT was consistent with the trend of the variation of the anthocyanin content of mature period accumulation type germplasms, which were the key speed-limiting genes of these kinds of germplasms. In the mid-developmental accumulation type, the accumulation of anthocyanin in the mesocarp was consistent with the trend of expression of all structural genes. Among the four regulatory genes, only the expression level of PpMYB10.1 was high and the expression pattern was similar to the pattern of anthocyanin accumulation in the two types of red flesh germplasms during fruit developmental stages. 【Conclusion】According to the coloring pattern of peach fruit and the pattern of anthocyanin accumulation, eight red-flesh peaches could be divided into two types, namely mature period accumulation type and mid-developmental accumulation type. The PpCHS and PpUFGT genes were the key structural genes of mature period accumulation type accessions, while PpMYB10.1 played a key role in the synthesis of anthocyanin in all red-flesh peach germplasms.

Key words: red flesh peach, anthocyanin, anthocyanin synthesis related genes

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