库尔勒香梨果实发育成熟的糖代谢和呼吸代谢响应特征

doi:10.3864/j.issn.0578-1752.2016.17.013

Abstract

Abstract: 【Objective】The responding relationship of sugar metabolism and respiratory metabolism throughout the fruit development and ripening of Korla pear (Pyrus sinkiangensis Yu) was analyzed with an attempt to assess the effect of metabolic interaction on fruit sugar accumulation.【Method】Korla pears were sampled at 10-day intervals from 60-150 DPA days post anthesis and were sliced into pulps, cores and peels, then the 12 enzyme activities of sugar metabolism and 9 enzyme activities of respiratory metabolism as well as the quality, vertical and horizontal diameter, contents of starch and 4 soluble sugars, and respiration rates of different respiratory pathways were measured. The relationship between quality and sugar components, respiratory main pathway and the effect of clustered metabolic enzymes interaction on the sugars composition at different stages of fruit development and ripening were determined from the results of regression analysis, hierarchical clustering and principal component analysis for discussion and profile of the metabolic pathways and features of different fruit parts in Korla pear.【Result】EMP (Embden-Meyerhof-Parnas) is the respiratory main path of core. EMP and TCAC (Tricarboxyficacid cycle) alternately compose the respiratory main path of pulp. EMP is integrated with TCAC in the respiratory main path of fruit peel. Either core or pulp, the metabolic interaction between sugar metabolism and respiratory metabolism differs in 2 patterns at 90 DPA before and after, and differs at 120 DPA of peel, respectively. The enzymes of core involved in metabolism of starch, sucrose and sorbitol were clustered with the enzymes involved in metabolism of EMP, PPP (phosphopentose pathway) and CP (Cytochrome pathway) at maturation, as well as the hierarchical cluster of pulp grouped the enzymes involved in metabolism of starch, sucrose, EMP, TCAC, CP and AP (alternative pathway), and the cluster of peel grouped the enzymes involved in metabolism of sucrose, sorbitl, EMP, TCAC, CP and AP. The clustered enzyme activities increased dramatically or peaked that was relating significantly to the fructose and glucose accumulations of core and pulp, and relating to the increase of fructose and glucose with sorbitol conversion of fruit peel also.【Conclusion】Korla pear forms typical internal quality by sugar accumulation composed of principal components with fructose and glucose, which has a heterostructure presence of sugar composition and sweetness, that is the result of different metabolic profiles between sugar metabolism and respiratory metabolism respond progressively in different structures at different stages throughout the fruit development and ripening. GPI (glucose phosphate isomerase) and COX (Cytochrome oxidase) are key enzymes of common crosslink in sugar metabolism and respiratory metabolism at fruit maturation in three parts of Korla pear.

Key words: Korla pear, fruit development, sugar metabolism, respiratory metabolism, metabolic profiles

PAN Yan, MENG Xin-tao, CHE Feng-bin, XUE Su-lin, ZHANG Ting, ZHAO Shi-rong, LIAO Kang. Metabolic Profiles of Sugar Metabolism and Respiratory Metabolism of Korla Pear (Pyrus sinkiangensis Yu) Throughout Fruit Development and Ripening[J].Scientia Agricultura Sinica, 2016, 49(17): 3391-3412.

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Metabolic Profiles of Sugar Metabolism and Respiratory Metabolism of Korla Pear (Pyrus sinkiangensis Yu) Throughout Fruit Development and Ripening

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