Abstract: 【Objective】The microbes carried by corn stalk maybe had the potential impact on the nutrients absorption and utilization of nutrients and animal health, due to the large amount of feeding. The objective of the present study was to investigate the effect of ensiling and natural drying of corn stalk silage on its bacterium composition and abundance, and try to provide a theoretical basis for the utilization of corn stalk from the microbiological point of view. 【Method】 The effect of ensiling and natural drying on composition and abundance of bacterium of corn stalk was analyzed using MiSeq sequencing technology. 【Result】The results showed that, the overall number of OTUs detected by the analysis reached 10887 based on ≥ 97% nucleotide sequence identity between reads, and the rarefaction curve of each sample was flattening. These implied that the sequencing depth was sufficient to reflect the vast majority of bacterium in the samples. There were differences and similarities on the composition and abundance of bacterium during stuff, dried and silage samples of corn stalk. Flora constitute of dry corn stalks was the most complex, followed by corn stalk stuff and the silage. The numbers of Proteobacteria plylum, Gammaproteobacteria class, Enterobacteriales order, Enterobacteriaceae family in dry corn stalk were 96.53%, 90.67%, 81.42% and 81.42%, which were significantly increased compared with stuff of corn stalk (P＜0.05); while the numbers of Proteobacteria plylum, Gammaproteobacteria class, Enterobacteriales order, Enterobacteriaceae family in corn stalk silage were 55.93%, 37.51%, 25.03% and 25.03%, which were significantly decreased compared with stuff and dry corn stalk (P＜0.05). 4) The numbers of Firmicutes phylum, Bacilli class, Lactobacillales order, Lactobacillaceae family, and Pediococcus genus in corn stalk silage were 39.10%, 38.91%, 38.81%, 32.08% and 28.14%, which were significantly increased compared with stuff and dry corn stalk (P＜0.05); while the numbers of Firmicutes phylum, Bacilli class, Lactobacillales order, Lactobacillaceae family, and Pediococcus genus in corn stalk silage were only 2.47%, 2.45%, 2.39%, 1.55% and 1.32%. 【Conclusion】 There was significant difference on bacterium composition and abundance between dried and silage samples of corn stalk; the using method of ensiling was better than natural dried corn stalk. MiSeq sequencing technology could provide the information of composition and abundance of flora.

Key words: MiSeq, corn stalk, ensiling, natural drying, bacterium