Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (20): 4056-4063.doi: 10.3864/j.issn.0578-1752.2015.20.007

• PLANT PROTECTION • Previous Articles     Next Articles

Antifungal Activities of Penicillium minioluteum ZF1 and Its Metabolites to Fusarium graminearum

SU Qian-fu1,2, JIA Jiao2, MENG Ling-min2, LI Hong2, ZHANG Wei2, JIN Qi-ming2, CONG Bin1


  1. 1Plant Protection College, Shenyang Agricultural University, Shenyang 110866
    2Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin
  • Received:2015-04-13 Online:2015-10-20 Published:2015-10-20

Abstract: 【Objective】 Penicillium minioluteum ZF1 was isolated from the soil and identified as having the effect of biological control in previous studies. The objective of this study is to analyze the inhibition effect of the isolate and its culture filtrate with chemical fungicide to Fusarium graminearum, clarify the value of ZF1, and to offer a novel method for controlling the stalk rot and ear rot which are caused by F. graminearum. 【Method】 F. graminearum was inoculated at the mixed PDA and the growth speed was calculated. The effect of secondary metabolites on control of the mycelial growth of F. graminearum was known when cellophane removed and F. graminearum was inoculated. Fludioxonil, ZF1 culture filtrate and the two mixtures were compared each other, and their EC50 and EC95 were measured. The leaves at 5-leaf stage and kernels at milk-ripe stage were inoculated with P. minioluteum, and the process was observed. 【Result】 P. minioluteum ZF1 could obviously inhibit the mycelial growth of F. graminearum. The inhibition ratio reached 81.33%. The area of inhibited mycelial growth of F. graminearum was 16.21 cm2 compared with the control groups. The effect of secondary metabolite of P. minioluteum was inoculated on PDA plate removed cellophane which inhibited the mycelia growth of F. graminearum reached 55.46%. The filtrate of P. minioluteum which were diluted 10, 20, 50 and 100 folds inhibited the growth area of mycelia of F. graminearum were 81.04%, 64.46%, 22.67% and 1.12%, respectively. The mycelia of morphology were not changed when ZF1 culture filtrate was added in PDA. The mixture of fludioxonil and 10% culture filtrate of P. minioluteum had the better effect than each single. The EC50 and EC95 values of fludioxonil to F. graminearum were 0.0162 and 0.5287 μg·mL-1, respectively. The inhibition ratios of 10% culture filtrate of P. minioluteum and 0.5 μg·mL-1 fludioxonil were 86.45% and 95.13%, respectively. EC50 and EC95 values of the two mixtures were 0.0023 and 0.4011 μg·mL-1. The effect of fludioxonil inhibition was lost after 4 days. The inhibition effect of the mixture of 0.5 μg·mL-1 fludioxonil and 10% culture filtrate of P. minioluteum prolonged for 10 days compared with only 0.5 μg·mL-1 fludioxonil. Slight mold appeared in injured maize kernels and seedling leaves when P. minioluteum was inoculated on them. 【Conclusion】 The inhibition effect of P. minioluteum ZF1 and its culture filtrate on F. graminearum was obvious. The mixture of fludioxonil and culture filtrate of P. minioluteum could prolong the inhibition time to the mycelial growth of F. graminearum. A novel method that P. minioluteum acts as a biocontrol fungus for controlling maize stalk rot and ear rot can be used in the future.

Key words: Penicillium, secondary metabolites, fludioxonil, antifungal activity, Fusarium graminearum

[1]    Dean R, Van Kan A L, Pretorius Z A, Hammond-Kosack K, Pietro A D, Spanu P D, Rudd J J, Dickman M, Kahmann R, Ellis J, Foster G D. The top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology, 2012, 13(4): 414-430.
[2]    李文娟, 何萍, 金继运. 钾素对玉米茎髓和幼根超微结构的影响及其与茎腐病抗性的关系. 中国农业科学, 2010, 43(4): 729-736.
Li W J, He P, Jin J Y. Effect of potassium on ultrastructure of maize stalk pith and young root and their relation to resistance to stalk rot. Scientia Agricultura Sinica, 2010, 43(4): 729-736. (in Chinese)
[3]    李文娟, 何萍, 金继运. 钾素对玉米茎腐病抗性反应中糖类物质代谢的影响. 植物营养与肥料学报, 2011, 17(1): 55-61.
Li W J, He P, Jin J Y. Effect of potassium on sugarmetabolism in resistant response to corn stalk rot. Plant Nutrition and Fertilizer Science, 2011, 17(1): 55-61. (in Chinese)
[4]    Reid L M, Nixol R W, Ouellet T, Savard M, Miller J D, Young J C, Stewart D W, Schaafsma A W. Interaction of Fusarium graminearum and F. moniliforme in maize ears: disease progress, fungal biomass, and mycotoxin accumulation. Phytopathology, 1999, 89(11): 1028-1037.
[5]    曾金凤. 青霉菌Z88菌株对水稻纹枯病菌的抗生作用. 福建农业大学学报, 1995, 24(2): 180-183.
Zeng J F. Antibiosis of the blue mold strain Z88 on Rhizoctonia solani. Journal of Fujian Agricultural University, 1995, 24(2): 180-183. (in Chinese)
[6]    陈杰, 郭天文, 汤琳, 谭雪莲, 朱渭兵, 薛泉宏. 灰黄青霉CF3对马铃薯土传病原真菌的拮抗性及其促生作用. 植物保护学报, 2013, 40(4): 301-308.
Chen J, Guo T W, Tang L, Tan X L, Zhu W B, Xue Q H. The antagonism of Penicillium griseofulvum CF3 against soil-borne fungal pathogens of potato and its growth-promoting effect on the plant. Acta Phytophylacica Sinica, 2013, 40(4): 301-308. (in Chinese)
[7]    马艳, 周明国, 常志洲, 张建英, 徐跃定. 生防青霉Penicillium striatisporum Pst10抗菌物质的分离纯化及生物活性测定. 江苏农业学报, 2013, 29(5): 1011-1018.
Ma Y, Zhou M G, Chang Z Z, Zhang J Y, Xu Y D. Purification, identification and bioactivity of antagonistic chemicals produced by biocontrol agents of Penicillium striatisporum Pst10. Jiangsu Journal of Agricultural Sciences, 2013, 29(5): 1011-1018. (in Chinese)
[8]    王霞, 王素英, 高朋辉. 青霉TS67菌株对大豆根腐病和玉米小斑病的防治效果评价. 微生物学通报, 2008, 35(8): 1246-1250.
Wang X, Wang S Y, Gao P H. Evaluation of antagonism of Penicillium TS67 against soybean root rot disease and corn southern leaf blight. Microbiology, 2008, 35(8): 1246-1250. (in Chinese)
[9]    陈安徽, 李春如, 樊美珍. 蝉拟青霉代谢产物清除DPPH自由基和抗真菌活性的研究. 菌物学报, 2008, 27(3): 405-412.
Chen A H, Li C R, Fan M Z. Scavenging and anti-fungal activities of the metabolite of Paecilomyces cicadae. Mycosystema, 2008, 27(3): 405-412. (in Chinese)
[10]   汪军, 王国芬, 杨腊英, 郭立佳, 潘江禹, 毛超, 戴青冬, 黄俊生. 施用淡紫拟青霉与套作对香蕉枯萎病控病作用的影响. 果树学报, 2013, 30(5): 857-864.
Wang J, Wang G F, Yang L Y, Guo L J, Pan J Y, Mao C, Dai D Q, Huang J S. Effects of Paecilomyces lilacinus application and intercropping on controlling Fusarium wilt of banana. Journal of Fruit Science, 2013, 30(5): 857-864. (in Chinese)
[11]   王淑媛, 王素英, 涂敏勤. 青霉TS67菌株活性产物的抗真菌作用. 微生物学通报, 2008, 35(9): 1404-1408.
Wang S Y, Wang S Y, Tu M Q. The antifungal mechanism of bioactive metabolites produced by Penicillum sp. TS67. Microbiology, 2008, 35(9): 1404-1408. (in Chinese)
[12]   李芳, 史怀, 刘波, 陈家骅. 淡紫拟青霉对尖孢镰刀菌的拮抗作用与机制分析. 植物保护学报, 2005, 32(4): 373-378.
Li F, Shi H, Liu B, Chen J H. Antagonistc effect of biocontrol fungus, Paecilomyces lilacinus strain NH-PL-03 and its mechanism against Fusarium oxysporum. Acta Phytophylacica Sinica, 2005, 32(4): 373-378. (in Chinese)
[13]   De Cal A, Garcia-Lepe R, Melgarejo P. Induced resistance by Penicillium oxalicum against Fusarium oxysporum f. sp. lycopersici: histological studies of infected and induced tomato stems. Phytopathology, 2000, 90(3): 260-268.
[14]   Sabuquillo P, De Cal A, Melgarejo P. Dispersal improvement of a powder formulation of Penicillium oxalicum, a biocontrol agent of tomato wilt. Plant Disease, 2005, 89(12): 1317-1323.
[15]   彭霞薇, 谢响明, 白志辉, 张洪勋. 草酸青霉BZH-2002果胶酶系的纯化及其诱导抗病作用. 应用与环境生物学报, 2006, 12(6): 750-753.
Peng X W, Xie X M, Bai Z H, Zhang H X. Purification and induced resistance of pectinases from Penicillium oxalicum BZH-2002. Chinese Journal of Applied and Environmental Biology, 2006, 12(6): 750-753. (in Chinese)
[16]   柴一秋, 金轶伟, 刘又高, 历晓腊, 陈官菊, 王根锷. 蝉拟青霉杀虫活性成分的分离. 中国农业科学, 2007, 40(9): 1952-1958.
Chai Y Q, Jin Y W, Liu Y G, Li X L, Chen G J, Wang G E. Separation of insecticidal ingredient of Paecilomyces cicadae (Miquel) Samson. Scientia Agricultura Sinica, 2007, 40(9): 1952-1958. (in Chinese)
[17]   焦豫良, 王淑军, 吕明生. 右旋糖酐-α-1, 6键水解酶的家族结构及其应用. 中国生物化学与分子生物学报, 2012, 28(11): 989-995.
Jiao Y L, Wang S J, Lü M S. Structure and function of dextran-α-1, 6-glucosidic bond hudrolase families and its application. Chinese Journal of Biochemistry and Molecular Biology, 2012, 28(11): 989-995. (in Chinese)
[18]   Betancourt L H, García R, González J, Montesino R, Quintero O, Takao T, Shimonishi Y, Cremata J A. Dextranase (α-1,6 glucan-6- glucanohydrolase) from Penicillium minioluteum expressed in Pichia pastoris: two host cells with minor differences in N-glycosylation. FEMS Yeast Research, 2001, 1(2): 151-160.
[19]   Li X M, Millson S H, Coker R D, Evans I H. Cloning and expression of Penicillium minioluteum dextranase on Saccharomyces cerevisiae and its exploitation as a reporter in the detection of mycotoxins. Biotechnology Letters, 2006, 28(23): 1955-1964.
[20]   Okada H, Kamiya S, Shiina Y, Suwa H, Nagashima M, Nakajima, S, Shimokawa H, Sugiyama E, Kondo H, Kojiri K, Suda H. BE-31405, a new antifungal antibiotic produced by Penicillium minioluteum. I. Description of producing organism, fermentation, isolation, physico- chemical and biological properties. The Journal of Atibiotics, 1998, 51(12): 1081-1086.
[21]   Yenn T W, Ngim A S, Ibrahim D, Zakaria L. Antimicrobial activity of Penicillium minioluteum ED24, an endophytic fungus residing in Orthosiphon Stamineus benth. World Journal of Pharmacy and Pharmaceutical Sciences, 2014, 3(3): 121-132.
[22]   Yang L P, Xie J T, Jiang D H, Fu Y P, Li G Q, Lin F C. Antifungal substances produced by Penicillium oxalicum strain PY-1—potential antibiotics against plant pathogenic fungi. World Journal of Microbiology and Biotechnology, 2008, 24(7): 909-915.
[23]   Dubey M K, Ubhayasekera W, Sandgren M, Jensen D F, Karlsson M. Disruption of the eng18B ENGase gene in the fungal biocontrol agent Trichoderma atroviride affects growth, conidiation and antagonistic ability.Plos One, 2012, 7(5): e36152.
[24]   Louw J P, Korsten L. Pathogenicity and host susceptibility of Penicillium spp. on citrus. Plant Disease, 2015, 99(1): 21-30.
[25]   Mansfield M A, Jones A D, Kuldau G A. Contamination of fresh and ensiled maize by multiple Penicillium mycotoxins. Phytopathology, 2008, 98(3): 330-336.
[26]   汪茜, 胡春锦, 柯仿钢, 黄思良, 黎起秦. 生防菌株1404的鉴定及其对采后柑橘炭疽病的防治效果. 微生物学报, 2010, 50(9): 1208-1217.
Wang Q, Hu C J, Ke F G, Huang S L, Li Q Q. Characterization of a bacterial biocontrol strain 1404 and its efficacy in controlling postharvest citrus anthracnose. Acta Microbiologica Sinica, 2010, 50(9): 1208-1217. (in Chinese)
[27]   刘颖超, 张金林, 庞民好, 张凤国, 张利辉. 咯菌腈对草莓灰霉病Botryt is cinerea的毒力及防效研究初报. 农药学学报, 2002, 4(3): 94-96.
Liu Y C, Zhang J L, Pang M H, Zhang F G, Zhang L H. Effect of fludioxonil against Botrytis cinerea on strawberry. Chinese Journal of Pesticide Science, 2002, 4(3): 94-96. (in Chinese)
[28]   Munkvold G P, O’Mara J K. Laboratory and growth chamber evaluation of fungicidal seed treatments for maize seedling blight caused by Fusarium species. Plant Disease, 2002, 86(2): 143-150.
[29]   Broders K D, Lipps P E, Paul P A, Dorrance A E. Evaluation of Fusarium graminearum associated with corn and soybean seed and seedling disease in Ohio. Plant Disease, 2007, 91(9): 1155-1160.
[30]   张婷, 衣思瑶, 武向文, 陈捷. 农药与木霉菌粉剂复配包衣种子对玉米出苗与生长的影响. 上海交通大学学报: 农业科学版, 2011, 29(4): 61-66.
Zhang T, Yi S Y, Wu X W, Chen J. Effect of seed coated with complex formulation of pesticides and Trichoderma biomass on maize emergence and seedling growth. Journal of Shanghai Jiaotong University: Agricultural Science, 2011, 29(4): 61-66. (in Chinese)
[1] GONG AnDong, ZHU ZiYu, LU YaNan, WAN HaiYan, WU NanNan, Cheelo Dimuna, GONG ShuangJun, WEN ShuTing, HOU Xiao. Functional Analysis of Burkholderia pyrrocinia WY6-5 on Phosphate Solubilizing, Antifungal and Growth-Promoting Activity of Maize [J]. Scientia Agricultura Sinica, 2019, 52(9): 1574-1586.
[2] ZHOU Zhi,LIU Yang,ZHANG LiMing,XU RuiNeng,SUN LiLi,LIAO Hong. Soil Nutrient Status in Wuyi Tea Region and Its Effects on Tea Quality-Related Constituents [J]. Scientia Agricultura Sinica, 2019, 52(8): 1425-1434.
[3] HE LiFei, CHEN LeLe, XIAO Bin, ZHAO ShiFeng, LI XiuHuan, MU Wei, LIU Feng. Establishment of Sensitivity Baseline and Evaluation of Field Control Efficacy of Fludioxonil Against Fulvia fulva [J]. Scientia Agricultura Sinica, 2018, 51(8): 1475-1483.
[4] WEI XinYan, HUANG YuanYuan, HUANG YaLi, DU KeJiu. Antagonism of Bacillus methylotrophicus Strain BH21 to Botrytis cinerea [J]. Scientia Agricultura Sinica, 2018, 51(5): 883-892.
[5] GONG ChangWei,QIN YiMan,QU JinSong,WANG XueGui. Resistance Detection and Mechanism of Strawberry Botrytis cinerea to Fludioxonil in Sichuan Province [J]. Scientia Agricultura Sinica, 2018, 51(22): 4277-4287.
[6] HOU Rui, WANG ChenFang. The Function of the Carbon Metabolism Regulator FgCreA in Fusarium graminearum [J]. Scientia Agricultura Sinica, 2018, 51(2): 257-267.
[7] MA HongXia, SUN Hua, GUO Ning, ZHANG HaiJian, SHI Jie, CHANG JiaYing. Analysis of Toxigenic Chemotype and Genetic Diversity of the Fusarium graminearum Species Complex [J]. Scientia Agricultura Sinica, 2018, 51(1): 82-95.
[8] XU JianQiang, PING ZhongLiang, LIU Ying, MA ShiChuang, XU DaoChao, YANG Lan, ZHENG Wei, LIU ShengMing, XIA YanFei, LIN XiaoMin. Inhibitory Activity of Fludioxonil to Four Pathogenic Fungi of Peony Leaves [J]. Scientia Agricultura Sinica, 2017, 50(20): 4036-4045.
[9] XIANG Ya-ping, CHEN Zhi-yi, LUO Chu-ping, ZHOU Hua-fei, LIU Yong-feng. The Antifungal Activities of Bacillus spp. and Its Relationship with Lipopeptide Antibiotics Produced by Bacillus spp. [J]. Scientia Agricultura Sinica, 2015, 48(20): 4064-4076.
[10] LIU Tai-guo, QIU Jun, ZHOU Yi-lin, XU Shi-chang, CHEN Huai-gu, LIU Yan, GAO Li, LIU Bo, ZHENG Chuan-lin, CHEN Wan-quan. Multi-Disease Resistance Evaluation of Chinese Advanced Winter Wheat Lines for the National Regional Test [J]. Scientia Agricultura Sinica, 2015, 48(15): 2967-2975.
[11] SHI Jian-rong, LIU Xin, QIU Jian-bo, JI Fang, XU Jian-hong, DONG Fei, YIN Xian-chao, RAN Jun-jian. Deoxynivalenol Contamination in Wheat and Its Management [J]. Scientia Agricultura Sinica, 2014, 47(18): 3641-3654.
[12] HU Lin-Gang, LI Jian-Peng, LI Yong-Cai, BI Yang, GE Yong-Hong, WANG Yi. Control of Exogenous H2O2 on Dry Rot of Potato Tuber and Possible Mechanism of Action [J]. Scientia Agricultura Sinica, 2013, 46(22): 4745-4752.
[13] TENG Yun,YU Zhi-yi,CUI Wen-hua,ZHANG Xin-gang,QUAN Xin,SUN Qiu,HOU Tai-ping
. An Antifungal Active Component from Spiraea alpina to Plant Fungi#br# [J]. Scientia Agricultura Sinica, 2009, 42(7): 2380-2385 .
[14] . Cloning and Expression of a Neutral Protein Gene BS2 from Bacillus subtilis B111 in Pichia pastoris
[J]. Scientia Agricultura Sinica, 2009, 42(3): 876-883 .
[15] . Stability of Antifungal Substance Produced by Actinomycete CCTCC M207210 and Its Application
[J]. Scientia Agricultura Sinica, 2009, 42(2): 636-641 .
Full text



No Suggested Reading articles found!