小麦新品种周麦23号的遗传构成分析及其特异引物筛选

doi:10.3864/j.issn.0578-1752.2015.19.016

Abstract

Abstract: 【Objective】 The objective of this study was to understand the genetic contributions of two parents to wheat variety Zhoumai 23 and the genetic composition of Zhoumai 23 in the Huang-Huai region, and the specific primers screened for the detection of the varietal authenticity of Zhoumai 23.【Method】 A total of 340 SSR markers covering 21 wheat chromosomes were used in a simple sequence repeat (SSR) markers analysis. Parsed the transfer frequency and genetic contribution rate of parental genetic material to Zhoumai 23. This also could be screened for several primers of Zhoumai 23 different from either parent, further screening of these specific markers using sister lines, derived varieties of Zhoumai 23. Finally, with the selected 1-2 specific primers of Zhoumai 23, and confirmed final screening of specific primers using Zhoumai 22, Jimai 22, Aikang 58, Zhengmai 366, a total of 14 materials of the main push varieties in the Huanghuai wheat region.【Result】 The results indicated that there were large differences in the genetic contribution from double parents to Zhoumai 23, the rate of the genetic contribution from Zhoumai 13 was 63.04%, much higher than that (36.96%) from Xinmai 9. Partial segregation occurred during the breeding of the parent material in the Zhoumai 23. The genetic contribution rate changes greatly in the different genomes and the chromosome level. The genetic contribution range from Zhoumai 13 to Zhoumai 23 was 23.1% (1B)-100% (4A, 6A, 3B, 4B, 6B, 4D) and from Xinmai 9 to Zhoumai 23 was 0 (4A, 6A, 3B, 4B, 6B, 4D)-76.9% (1B). The study identified seven specific sites in Zhoumai 23 from 147 polymorphic markers, named Xwmc344, Xbarc84, Xwmc326, Xwmc468, Xwmc479, Xgwm428 and Xcwm65. One specific primer, Xcwm65, can be used to differentiate Zhoumai 23 from the main varieties in Huang-Huai wheat region. Sublines A3, A4, A5 and derived varieties B7, B8, B12, and B15 of Zhoumai 23 were still difficult to distinguish. Therefore, it is needed to identify the different molecular markers to distinguish between Zhoumai 23 sister lines and its derivatives. 【Conclusion】 The study revealed a genetic contribution from the parents to Zhoumai 23, master of Zhoumai 23 genetic composition and the mapping of the gene map, and found a specific primer for the identified authenticity of Zhoumai 23.

Key words: common wheat, Zhoumai 23, genetic constitution, specific primer

ZOU Shao-kui, YIN Gui-hong, TANG Jian-wei, HAN Yu-lin, LI Nan-nan, LI Shun-cheng, HUANG Feng, WANG Li-na, ZHANG Qian, GAO Yan. Genetic Analysis of New Wheat Variety Zhoumai 23 and Screening of Specific Primers[J].Scientia Agricultura Sinica, 2015, 48(19): 3941-3951.

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